Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4768 and Pf1N1B4_4769 are separated by 253 nucleotides Pf1N1B4_4769 and Pf1N1B4_4770 are separated by 118 nucleotides Pf1N1B4_4770 and Pf1N1B4_4771 are separated by 101 nucleotides Pf1N1B4_4771 and Pf1N1B4_4772 are separated by 190 nucleotides
Pf1N1B4_4768 - hypothetical protein, at 5,264,824 to 5,267,631
_4768
Pf1N1B4_4769 - hypothetical protein, at 5,267,885 to 5,268,076
_4769
Pf1N1B4_4770 - Putative autotransporter, at 5,268,195 to 5,270,408
_4770
Pf1N1B4_4771 - Probable transmembrane protein, at 5,270,510 to 5,271,151
_4771
Pf1N1B4_4772 - RND efflux system, inner membrane transporter CmeB, at 5,271,342 to 5,274,440
_4772
Position (kb)
5268
5269
5270
5271 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5267.257 kb on + strand, within Pf1N1B4_4768 at 5267.274 kb on + strand, within Pf1N1B4_4768 at 5267.288 kb on + strand, within Pf1N1B4_4768 at 5267.362 kb on - strand at 5267.413 kb on + strand at 5267.413 kb on + strand at 5267.413 kb on + strand at 5267.418 kb on + strand at 5267.418 kb on + strand at 5267.453 kb on + strand at 5267.453 kb on + strand at 5267.515 kb on + strand at 5267.523 kb on + strand at 5267.523 kb on + strand at 5267.523 kb on + strand at 5267.634 kb on - strand at 5267.795 kb on - strand at 5267.850 kb on + strand at 5267.959 kb on - strand, within Pf1N1B4_4769 at 5268.275 kb on + strand at 5268.275 kb on + strand at 5268.276 kb on - strand at 5268.276 kb on - strand at 5268.508 kb on + strand, within Pf1N1B4_4770 at 5268.710 kb on + strand, within Pf1N1B4_4770 at 5268.711 kb on - strand, within Pf1N1B4_4770 at 5268.711 kb on - strand, within Pf1N1B4_4770 at 5268.828 kb on - strand at 5268.831 kb on - strand, within Pf1N1B4_4770 at 5268.831 kb on - strand, within Pf1N1B4_4770 at 5268.843 kb on - strand, within Pf1N1B4_4770 at 5268.847 kb on + strand, within Pf1N1B4_4770 at 5268.847 kb on + strand, within Pf1N1B4_4770 at 5268.847 kb on + strand, within Pf1N1B4_4770 at 5268.848 kb on - strand, within Pf1N1B4_4770 at 5268.848 kb on - strand, within Pf1N1B4_4770 at 5268.848 kb on - strand, within Pf1N1B4_4770 at 5268.848 kb on - strand, within Pf1N1B4_4770 at 5269.184 kb on + strand, within Pf1N1B4_4770 at 5269.185 kb on - strand, within Pf1N1B4_4770 at 5269.383 kb on - strand, within Pf1N1B4_4770 at 5269.422 kb on + strand, within Pf1N1B4_4770 at 5269.642 kb on - strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.948 kb on - strand, within Pf1N1B4_4770 at 5269.991 kb on + strand, within Pf1N1B4_4770 at 5270.010 kb on + strand, within Pf1N1B4_4770 at 5270.010 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.035 kb on + strand, within Pf1N1B4_4770 at 5270.267 kb on + strand at 5270.315 kb on + strand at 5270.315 kb on + strand at 5270.315 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.397 kb on + strand at 5270.398 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.745 kb on + strand, within Pf1N1B4_4771 at 5270.745 kb on + strand at 5270.746 kb on - strand, within Pf1N1B4_4771 at 5270.748 kb on + strand, within Pf1N1B4_4771 at 5270.748 kb on + strand at 5270.749 kb on - strand, within Pf1N1B4_4771 at 5270.749 kb on - strand at 5270.760 kb on + strand, within Pf1N1B4_4771 at 5270.761 kb on - strand, within Pf1N1B4_4771 at 5270.927 kb on + strand at 5270.927 kb on + strand at 5270.927 kb on + strand, within Pf1N1B4_4771 at 5271.177 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 5,267,257 + Pf1N1B4_4768 0.87 +0.8 5,267,274 + Pf1N1B4_4768 0.87 -1.8 5,267,288 + Pf1N1B4_4768 0.88 +0.2 5,267,362 - +1.1 5,267,413 + -1.5 5,267,413 + -1.1 5,267,413 + -0.1 5,267,418 + -1.2 5,267,418 + -1.8 5,267,453 + -1.8 5,267,453 + +0.1 5,267,515 + -1.4 5,267,523 + +0.8 5,267,523 + +0.5 5,267,523 + -0.8 5,267,634 - -0.2 5,267,795 - +0.2 5,267,850 + -0.8 5,267,959 - Pf1N1B4_4769 0.39 -0.2 5,268,275 + -0.8 5,268,275 + +1.2 5,268,276 - -3.1 5,268,276 - +0.5 5,268,508 + Pf1N1B4_4770 0.14 +1.8 5,268,710 + Pf1N1B4_4770 0.23 -0.8 5,268,711 - Pf1N1B4_4770 0.23 -2.0 5,268,711 - Pf1N1B4_4770 0.23 +1.7 5,268,828 - +0.2 5,268,831 - Pf1N1B4_4770 0.29 +0.5 5,268,831 - Pf1N1B4_4770 0.29 -0.2 5,268,843 - Pf1N1B4_4770 0.29 +0.4 5,268,847 + Pf1N1B4_4770 0.29 -2.1 5,268,847 + Pf1N1B4_4770 0.29 +0.5 5,268,847 + Pf1N1B4_4770 0.29 -0.0 5,268,848 - Pf1N1B4_4770 0.29 -0.4 5,268,848 - Pf1N1B4_4770 0.29 -1.8 5,268,848 - Pf1N1B4_4770 0.29 -0.5 5,268,848 - Pf1N1B4_4770 0.29 -0.2 5,269,184 + Pf1N1B4_4770 0.45 +1.2 5,269,185 - Pf1N1B4_4770 0.45 +0.3 5,269,383 - Pf1N1B4_4770 0.54 -1.4 5,269,422 + Pf1N1B4_4770 0.55 -1.8 5,269,642 - Pf1N1B4_4770 0.65 +0.1 5,269,947 + Pf1N1B4_4770 0.79 +0.8 5,269,947 + Pf1N1B4_4770 0.79 -1.4 5,269,947 + Pf1N1B4_4770 0.79 -1.8 5,269,947 + Pf1N1B4_4770 0.79 +1.2 5,269,948 - Pf1N1B4_4770 0.79 -3.1 5,269,991 + Pf1N1B4_4770 0.81 -2.1 5,270,010 + Pf1N1B4_4770 0.82 -1.8 5,270,010 + Pf1N1B4_4770 0.82 -1.2 5,270,029 + Pf1N1B4_4770 0.83 -1.8 5,270,029 + +0.8 5,270,029 + Pf1N1B4_4770 0.83 -1.4 5,270,029 + Pf1N1B4_4770 0.83 -2.8 5,270,029 + Pf1N1B4_4770 0.83 -3.4 5,270,035 + Pf1N1B4_4770 0.83 -2.4 5,270,267 + -0.5 5,270,315 + -0.2 5,270,315 + -0.4 5,270,315 + -0.8 5,270,386 + -0.1 5,270,386 + +2.4 5,270,386 + +2.3 5,270,386 + -0.8 5,270,397 + -1.5 5,270,398 - +0.3 5,270,561 - -1.8 5,270,561 - -0.8 5,270,561 - -0.2 5,270,561 - +1.0 5,270,561 - +2.5 5,270,745 + Pf1N1B4_4771 0.37 +0.2 5,270,745 + -1.8 5,270,746 - Pf1N1B4_4771 0.37 -1.2 5,270,748 + Pf1N1B4_4771 0.37 +0.2 5,270,748 + +0.2 5,270,749 - Pf1N1B4_4771 0.37 +0.8 5,270,749 - +0.5 5,270,760 + Pf1N1B4_4771 0.39 +0.8 5,270,761 - Pf1N1B4_4771 0.39 -0.8 5,270,927 + -1.8 5,270,927 + +0.5 5,270,927 + Pf1N1B4_4771 0.65 +0.0 5,271,177 - +0.8
Or see this region's nucleotide sequence