Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4725 and Pf1N1B4_4726 overlap by 4 nucleotides Pf1N1B4_4726 and Pf1N1B4_4727 overlap by 4 nucleotides Pf1N1B4_4727 and Pf1N1B4_4728 are separated by 115 nucleotides
Pf1N1B4_4725 - 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44), at 5,221,181 to 5,222,161
_4725
Pf1N1B4_4726 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 5,222,158 to 5,223,681
_4726
Pf1N1B4_4727 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 5,223,678 to 5,224,499
_4727
Pf1N1B4_4728 - Na+/H+ antiporter NhaB, at 5,224,615 to 5,226,117
_4728
Position (kb)
5222
5223
5224 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5221.162 kb on + strand at 5221.166 kb on - strand at 5221.178 kb on + strand at 5221.178 kb on + strand at 5221.178 kb on + strand at 5221.178 kb on + strand at 5221.179 kb on - strand at 5221.179 kb on - strand at 5221.222 kb on + strand at 5221.252 kb on + strand at 5221.253 kb on - strand at 5221.265 kb on + strand at 5221.423 kb on + strand, within Pf1N1B4_4725 at 5221.423 kb on + strand, within Pf1N1B4_4725 at 5221.424 kb on - strand, within Pf1N1B4_4725 at 5221.472 kb on + strand, within Pf1N1B4_4725 at 5221.472 kb on + strand, within Pf1N1B4_4725 at 5221.472 kb on + strand, within Pf1N1B4_4725 at 5221.473 kb on - strand at 5221.473 kb on - strand, within Pf1N1B4_4725 at 5221.475 kb on - strand, within Pf1N1B4_4725 at 5221.572 kb on - strand, within Pf1N1B4_4725 at 5222.269 kb on + strand at 5222.269 kb on + strand at 5222.270 kb on - strand at 5222.270 kb on - strand at 5222.270 kb on - strand at 5222.462 kb on - strand, within Pf1N1B4_4726 at 5222.462 kb on - strand, within Pf1N1B4_4726 at 5222.548 kb on + strand, within Pf1N1B4_4726 at 5222.746 kb on + strand, within Pf1N1B4_4726 at 5222.747 kb on - strand, within Pf1N1B4_4726 at 5222.943 kb on - strand, within Pf1N1B4_4726 at 5223.160 kb on + strand, within Pf1N1B4_4726 at 5223.160 kb on + strand, within Pf1N1B4_4726 at 5223.161 kb on - strand, within Pf1N1B4_4726 at 5223.215 kb on - strand, within Pf1N1B4_4726 at 5223.215 kb on - strand at 5223.215 kb on - strand, within Pf1N1B4_4726 at 5223.328 kb on + strand, within Pf1N1B4_4726 at 5223.329 kb on - strand, within Pf1N1B4_4726 at 5223.484 kb on + strand, within Pf1N1B4_4726 at 5223.484 kb on + strand at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.910 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.943 kb on - strand, within Pf1N1B4_4727 at 5224.119 kb on + strand at 5224.242 kb on + strand, within Pf1N1B4_4727 at 5224.242 kb on + strand, within Pf1N1B4_4727 at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.617 kb on - strand at 5224.617 kb on - strand at 5224.619 kb on - strand at 5224.626 kb on - strand at 5224.638 kb on - strand at 5224.665 kb on + strand at 5224.666 kb on - strand at 5224.666 kb on - strand at 5224.668 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 5,221,162 + -0.8 5,221,166 - -0.4 5,221,178 + -2.8 5,221,178 + +0.8 5,221,178 + -0.8 5,221,178 + +1.9 5,221,179 - +3.0 5,221,179 - +0.8 5,221,222 + +1.7 5,221,252 + +1.2 5,221,253 - +1.5 5,221,265 + +0.4 5,221,423 + Pf1N1B4_4725 0.25 -0.3 5,221,423 + Pf1N1B4_4725 0.25 -1.8 5,221,424 - Pf1N1B4_4725 0.25 -0.0 5,221,472 + Pf1N1B4_4725 0.30 +1.8 5,221,472 + Pf1N1B4_4725 0.30 +1.2 5,221,472 + Pf1N1B4_4725 0.30 -0.2 5,221,473 - -1.3 5,221,473 - Pf1N1B4_4725 0.30 +3.7 5,221,475 - Pf1N1B4_4725 0.30 +0.9 5,221,572 - Pf1N1B4_4725 0.40 +1.5 5,222,269 + -0.8 5,222,269 + +0.2 5,222,270 - +0.1 5,222,270 - -0.3 5,222,270 - +0.8 5,222,462 - Pf1N1B4_4726 0.20 +1.8 5,222,462 - Pf1N1B4_4726 0.20 -3.1 5,222,548 + Pf1N1B4_4726 0.26 +1.2 5,222,746 + Pf1N1B4_4726 0.39 -0.2 5,222,747 - Pf1N1B4_4726 0.39 -1.5 5,222,943 - Pf1N1B4_4726 0.52 -0.2 5,223,160 + Pf1N1B4_4726 0.66 -1.6 5,223,160 + Pf1N1B4_4726 0.66 +0.0 5,223,161 - Pf1N1B4_4726 0.66 +2.5 5,223,215 - Pf1N1B4_4726 0.69 -0.1 5,223,215 - -0.2 5,223,215 - Pf1N1B4_4726 0.69 +1.0 5,223,328 + Pf1N1B4_4726 0.77 -1.8 5,223,329 - Pf1N1B4_4726 0.77 -1.4 5,223,484 + Pf1N1B4_4726 0.87 +0.0 5,223,484 + -0.8 5,223,485 - Pf1N1B4_4726 0.87 -2.4 5,223,485 - Pf1N1B4_4726 0.87 -0.4 5,223,485 - Pf1N1B4_4726 0.87 -0.4 5,223,485 - Pf1N1B4_4726 0.87 +1.6 5,223,485 - Pf1N1B4_4726 0.87 -2.1 5,223,910 + Pf1N1B4_4727 0.28 -2.4 5,223,942 + Pf1N1B4_4727 0.32 -1.8 5,223,942 + Pf1N1B4_4727 0.32 +1.0 5,223,942 + Pf1N1B4_4727 0.32 +0.8 5,223,942 + Pf1N1B4_4727 0.32 -1.8 5,223,943 - Pf1N1B4_4727 0.32 +0.0 5,224,119 + -1.8 5,224,242 + Pf1N1B4_4727 0.69 -0.2 5,224,242 + Pf1N1B4_4727 0.69 -0.7 5,224,482 + -1.8 5,224,482 + -0.8 5,224,482 + +1.0 5,224,482 + -0.8 5,224,483 - +0.8 5,224,483 - -3.4 5,224,483 - -1.8 5,224,483 - -1.8 5,224,483 - -2.4 5,224,483 - +1.0 5,224,617 - -1.5 5,224,617 - -0.8 5,224,619 - -2.4 5,224,626 - -0.8 5,224,638 - -1.8 5,224,665 + +0.8 5,224,666 - -1.8 5,224,666 - -0.8 5,224,668 - -1.2
Or see this region's nucleotide sequence