Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4559 and Pf1N1B4_4560 are separated by 129 nucleotides Pf1N1B4_4560 and Pf1N1B4_4561 are separated by 184 nucleotides Pf1N1B4_4561 and Pf1N1B4_4562 are separated by 278 nucleotides
Pf1N1B4_4559 - Predicted exporter of the RND superfamily, at 5,028,595 to 5,031,210
_4559
Pf1N1B4_4560 - Transcriptional regulator, MerR family, at 5,031,340 to 5,031,723
_4560
Pf1N1B4_4561 - Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17), at 5,031,908 to 5,032,483
_4561
Pf1N1B4_4562 - Serine transporter, at 5,032,762 to 5,034,042
_4562
Position (kb) 5031
5032
5033 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5031.084 kb on - strand at 5031.186 kb on - strand at 5031.209 kb on - strand at 5031.302 kb on - strand at 5031.351 kb on + strand at 5031.477 kb on + strand, within Pf1N1B4_4560 at 5031.477 kb on + strand, within Pf1N1B4_4560 at 5031.477 kb on + strand, within Pf1N1B4_4560 at 5031.611 kb on + strand, within Pf1N1B4_4560 at 5031.611 kb on + strand, within Pf1N1B4_4560 at 5031.719 kb on + strand at 5031.720 kb on - strand at 5031.791 kb on + strand at 5031.843 kb on - strand at 5032.074 kb on + strand, within Pf1N1B4_4561 at 5032.075 kb on - strand, within Pf1N1B4_4561 at 5032.075 kb on - strand, within Pf1N1B4_4561 at 5032.077 kb on + strand, within Pf1N1B4_4561 at 5032.335 kb on - strand, within Pf1N1B4_4561 at 5032.410 kb on + strand, within Pf1N1B4_4561 at 5032.410 kb on + strand, within Pf1N1B4_4561 at 5032.410 kb on + strand, within Pf1N1B4_4561 at 5032.410 kb on + strand, within Pf1N1B4_4561 at 5032.410 kb on + strand, within Pf1N1B4_4561 at 5032.411 kb on - strand at 5032.411 kb on - strand, within Pf1N1B4_4561 at 5032.411 kb on - strand, within Pf1N1B4_4561 at 5032.414 kb on + strand, within Pf1N1B4_4561 at 5032.415 kb on - strand, within Pf1N1B4_4561 at 5032.439 kb on - strand at 5032.583 kb on - strand at 5032.635 kb on - strand at 5032.795 kb on + strand at 5032.796 kb on - strand at 5033.001 kb on - strand, within Pf1N1B4_4562 at 5033.087 kb on - strand at 5033.119 kb on + strand at 5033.208 kb on - strand, within Pf1N1B4_4562
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 5,031,084 - +0.4 5,031,186 - -1.2 5,031,209 - -0.6 5,031,302 - -0.6 5,031,351 + +1.5 5,031,477 + Pf1N1B4_4560 0.36 -0.0 5,031,477 + Pf1N1B4_4560 0.36 +0.7 5,031,477 + Pf1N1B4_4560 0.36 +1.1 5,031,611 + Pf1N1B4_4560 0.71 -2.2 5,031,611 + Pf1N1B4_4560 0.71 +0.4 5,031,719 + +0.6 5,031,720 - +1.6 5,031,791 + -1.8 5,031,843 - -0.0 5,032,074 + Pf1N1B4_4561 0.29 -1.2 5,032,075 - Pf1N1B4_4561 0.29 -3.4 5,032,075 - Pf1N1B4_4561 0.29 -3.2 5,032,077 + Pf1N1B4_4561 0.29 -0.6 5,032,335 - Pf1N1B4_4561 0.74 -2.6 5,032,410 + Pf1N1B4_4561 0.87 -1.0 5,032,410 + Pf1N1B4_4561 0.87 -0.9 5,032,410 + Pf1N1B4_4561 0.87 -1.6 5,032,410 + Pf1N1B4_4561 0.87 +0.1 5,032,410 + Pf1N1B4_4561 0.87 -1.6 5,032,411 - -1.6 5,032,411 - Pf1N1B4_4561 0.87 -1.6 5,032,411 - Pf1N1B4_4561 0.87 -0.6 5,032,414 + Pf1N1B4_4561 0.88 -0.3 5,032,415 - Pf1N1B4_4561 0.88 -0.6 5,032,439 - +1.0 5,032,583 - +0.4 5,032,635 - +1.0 5,032,795 + -2.9 5,032,796 - +0.7 5,033,001 - Pf1N1B4_4562 0.19 +1.4 5,033,087 - +1.0 5,033,119 + -0.6 5,033,208 - Pf1N1B4_4562 0.35 -0.3
Or see this region's nucleotide sequence