Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_44 and Pf1N1B4_45 are separated by 10 nucleotides Pf1N1B4_45 and Pf1N1B4_46 are separated by 55 nucleotides Pf1N1B4_46 and Pf1N1B4_47 are separated by 3 nucleotides
Pf1N1B4_44 - Multicopper oxidase, at 51,784 to 53,577
_44
Pf1N1B4_45 - Copper resistance protein B, at 53,588 to 54,478
_45
Pf1N1B4_46 - Copper resistance protein C precursor, at 54,534 to 54,917
_46
Pf1N1B4_47 - Copper resistance protein D, at 54,921 to 55,850
_47
Position (kb) 53
54
55 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 52.624 kb on + strand, within Pf1N1B4_44 at 52.765 kb on + strand at 52.765 kb on + strand, within Pf1N1B4_44 at 52.765 kb on + strand, within Pf1N1B4_44 at 52.766 kb on - strand, within Pf1N1B4_44 at 52.766 kb on - strand, within Pf1N1B4_44 at 53.177 kb on - strand, within Pf1N1B4_44 at 53.512 kb on + strand at 53.531 kb on - strand at 53.689 kb on + strand, within Pf1N1B4_45 at 53.816 kb on + strand at 53.861 kb on + strand, within Pf1N1B4_45 at 53.861 kb on + strand, within Pf1N1B4_45 at 53.861 kb on + strand at 53.861 kb on + strand at 53.861 kb on + strand, within Pf1N1B4_45 at 53.861 kb on + strand at 53.862 kb on - strand at 53.862 kb on - strand, within Pf1N1B4_45 at 53.864 kb on + strand, within Pf1N1B4_45 at 53.864 kb on + strand, within Pf1N1B4_45 at 53.864 kb on + strand, within Pf1N1B4_45 at 53.864 kb on + strand, within Pf1N1B4_45 at 54.137 kb on + strand, within Pf1N1B4_45 at 54.138 kb on - strand, within Pf1N1B4_45 at 54.210 kb on - strand, within Pf1N1B4_45 at 54.365 kb on + strand at 54.365 kb on + strand, within Pf1N1B4_45 at 54.365 kb on + strand, within Pf1N1B4_45 at 54.365 kb on + strand, within Pf1N1B4_45 at 54.365 kb on + strand, within Pf1N1B4_45 at 54.365 kb on + strand, within Pf1N1B4_45 at 54.365 kb on + strand, within Pf1N1B4_45 at 54.366 kb on - strand, within Pf1N1B4_45 at 54.366 kb on - strand, within Pf1N1B4_45 at 54.366 kb on - strand, within Pf1N1B4_45 at 54.393 kb on - strand at 54.410 kb on + strand at 54.411 kb on - strand at 54.485 kb on + strand at 54.486 kb on - strand at 54.514 kb on - strand at 54.843 kb on + strand, within Pf1N1B4_46 at 54.843 kb on + strand, within Pf1N1B4_46 at 54.844 kb on - strand, within Pf1N1B4_46 at 54.844 kb on - strand, within Pf1N1B4_46 at 54.844 kb on - strand, within Pf1N1B4_46 at 54.844 kb on - strand, within Pf1N1B4_46 at 54.899 kb on + strand at 54.899 kb on + strand at 54.899 kb on + strand at 54.899 kb on + strand at 54.960 kb on + strand at 54.960 kb on + strand at 54.960 kb on + strand at 54.960 kb on + strand at 54.960 kb on + strand at 54.960 kb on + strand at 54.960 kb on + strand at 54.961 kb on - strand at 54.961 kb on - strand at 54.961 kb on - strand at 54.961 kb on - strand at 54.961 kb on - strand at 55.006 kb on - strand at 55.006 kb on - strand at 55.014 kb on - strand, within Pf1N1B4_47 at 55.276 kb on + strand, within Pf1N1B4_47 at 55.277 kb on - strand, within Pf1N1B4_47 at 55.341 kb on - strand, within Pf1N1B4_47
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 52,624 + Pf1N1B4_44 0.47 -1.8 52,765 + -1.2 52,765 + Pf1N1B4_44 0.55 +0.1 52,765 + Pf1N1B4_44 0.55 -3.0 52,766 - Pf1N1B4_44 0.55 -0.9 52,766 - Pf1N1B4_44 0.55 -0.8 53,177 - Pf1N1B4_44 0.78 +0.8 53,512 + +0.8 53,531 - -2.1 53,689 + Pf1N1B4_45 0.11 -0.9 53,816 + -0.2 53,861 + Pf1N1B4_45 0.31 +0.6 53,861 + Pf1N1B4_45 0.31 +1.2 53,861 + -1.2 53,861 + -0.2 53,861 + Pf1N1B4_45 0.31 +3.5 53,861 + -1.2 53,862 - +2.1 53,862 - Pf1N1B4_45 0.31 -1.0 53,864 + Pf1N1B4_45 0.31 +0.8 53,864 + Pf1N1B4_45 0.31 +0.2 53,864 + Pf1N1B4_45 0.31 -0.8 53,864 + Pf1N1B4_45 0.31 -0.2 54,137 + Pf1N1B4_45 0.62 -0.4 54,138 - Pf1N1B4_45 0.62 -1.2 54,210 - Pf1N1B4_45 0.70 -3.7 54,365 + -1.2 54,365 + Pf1N1B4_45 0.87 -0.2 54,365 + Pf1N1B4_45 0.87 -1.2 54,365 + Pf1N1B4_45 0.87 -1.5 54,365 + Pf1N1B4_45 0.87 -0.9 54,365 + Pf1N1B4_45 0.87 -1.5 54,365 + Pf1N1B4_45 0.87 +0.8 54,366 - Pf1N1B4_45 0.87 +1.0 54,366 - Pf1N1B4_45 0.87 -1.0 54,366 - Pf1N1B4_45 0.87 -0.3 54,393 - -0.2 54,410 + -0.8 54,411 - -0.2 54,485 + -0.2 54,486 - -0.2 54,514 - -1.8 54,843 + Pf1N1B4_46 0.80 -1.4 54,843 + Pf1N1B4_46 0.80 -0.2 54,844 - Pf1N1B4_46 0.81 +0.6 54,844 - Pf1N1B4_46 0.81 -2.8 54,844 - Pf1N1B4_46 0.81 -2.4 54,844 - Pf1N1B4_46 0.81 -1.2 54,899 + -0.2 54,899 + -1.8 54,899 + +0.1 54,899 + +0.4 54,960 + -0.5 54,960 + +0.2 54,960 + -0.5 54,960 + -1.2 54,960 + -1.2 54,960 + +2.0 54,960 + +1.0 54,961 - -0.2 54,961 - +0.4 54,961 - -1.9 54,961 - +1.2 54,961 - -1.8 55,006 - -0.2 55,006 - +1.2 55,014 - Pf1N1B4_47 0.10 -1.2 55,276 + Pf1N1B4_47 0.38 -0.2 55,277 - Pf1N1B4_47 0.38 -1.2 55,341 - Pf1N1B4_47 0.45 +0.6
Or see this region's nucleotide sequence