Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4325 and Pf1N1B4_4326 are separated by 4 nucleotides Pf1N1B4_4326 and Pf1N1B4_4327 overlap by 29 nucleotides Pf1N1B4_4327 and Pf1N1B4_4328 are separated by 26 nucleotides
Pf1N1B4_4325 - Agmatine deiminase (EC 3.5.3.12), at 4,785,715 to 4,786,767
_4325
Pf1N1B4_4326 - N-carbamoylputrescine amidase (3.5.1.53), at 4,786,772 to 4,787,680
_4326
Pf1N1B4_4327 - Spermidine/putrescine-binding periplasmic protein, at 4,787,652 to 4,788,785
_4327
Pf1N1B4_4328 - Agmatine deiminase (EC 3.5.3.12), at 4,788,812 to 4,789,936
_4328
Position (kb)
4787
4788
4789 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4786.917 kb on - strand, within Pf1N1B4_4326 at 4786.917 kb on - strand, within Pf1N1B4_4326 at 4786.917 kb on - strand, within Pf1N1B4_4326 at 4786.980 kb on + strand at 4787.061 kb on - strand, within Pf1N1B4_4326 at 4787.159 kb on + strand, within Pf1N1B4_4326 at 4787.160 kb on - strand, within Pf1N1B4_4326 at 4787.462 kb on + strand, within Pf1N1B4_4326 at 4787.616 kb on - strand at 4787.616 kb on - strand at 4787.779 kb on - strand, within Pf1N1B4_4327 at 4787.794 kb on - strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.043 kb on - strand at 4788.043 kb on - strand at 4788.072 kb on + strand, within Pf1N1B4_4327 at 4788.073 kb on - strand, within Pf1N1B4_4327 at 4788.099 kb on + strand at 4788.201 kb on - strand, within Pf1N1B4_4327 at 4788.201 kb on - strand, within Pf1N1B4_4327 at 4788.249 kb on + strand, within Pf1N1B4_4327 at 4788.250 kb on - strand, within Pf1N1B4_4327 at 4788.320 kb on - strand, within Pf1N1B4_4327 at 4788.327 kb on + strand, within Pf1N1B4_4327 at 4788.332 kb on - strand, within Pf1N1B4_4327 at 4788.543 kb on + strand, within Pf1N1B4_4327 at 4788.544 kb on - strand, within Pf1N1B4_4327 at 4788.672 kb on + strand at 4788.672 kb on + strand at 4788.673 kb on - strand at 4788.673 kb on - strand at 4788.690 kb on + strand at 4789.061 kb on + strand, within Pf1N1B4_4328 at 4789.139 kb on + strand, within Pf1N1B4_4328 at 4789.140 kb on - strand, within Pf1N1B4_4328 at 4789.268 kb on - strand, within Pf1N1B4_4328 at 4789.351 kb on + strand, within Pf1N1B4_4328 at 4789.755 kb on - strand, within Pf1N1B4_4328
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 4,786,917 - Pf1N1B4_4326 0.16 +0.7 4,786,917 - Pf1N1B4_4326 0.16 +0.5 4,786,917 - Pf1N1B4_4326 0.16 -0.3 4,786,980 + -1.1 4,787,061 - Pf1N1B4_4326 0.32 +1.1 4,787,159 + Pf1N1B4_4326 0.43 -1.2 4,787,160 - Pf1N1B4_4326 0.43 +1.6 4,787,462 + Pf1N1B4_4326 0.76 +1.7 4,787,616 - +0.5 4,787,616 - -2.1 4,787,779 - Pf1N1B4_4327 0.11 -0.8 4,787,794 - Pf1N1B4_4327 0.13 +0.7 4,787,850 + Pf1N1B4_4327 0.17 -2.1 4,787,850 + Pf1N1B4_4327 0.17 +0.1 4,787,850 + Pf1N1B4_4327 0.17 -1.1 4,787,850 + Pf1N1B4_4327 0.17 +1.3 4,788,042 + Pf1N1B4_4327 0.34 +0.4 4,788,042 + Pf1N1B4_4327 0.34 -0.1 4,788,042 + Pf1N1B4_4327 0.34 +0.2 4,788,042 + Pf1N1B4_4327 0.34 -0.5 4,788,043 - +0.1 4,788,043 - +1.4 4,788,072 + Pf1N1B4_4327 0.37 -1.2 4,788,073 - Pf1N1B4_4327 0.37 -1.5 4,788,099 + +1.5 4,788,201 - Pf1N1B4_4327 0.48 -3.1 4,788,201 - Pf1N1B4_4327 0.48 +0.5 4,788,249 + Pf1N1B4_4327 0.53 +0.5 4,788,250 - Pf1N1B4_4327 0.53 -0.5 4,788,320 - Pf1N1B4_4327 0.59 -0.9 4,788,327 + Pf1N1B4_4327 0.60 +1.3 4,788,332 - Pf1N1B4_4327 0.60 -0.9 4,788,543 + Pf1N1B4_4327 0.79 -0.5 4,788,544 - Pf1N1B4_4327 0.79 +0.6 4,788,672 + +1.3 4,788,672 + -2.1 4,788,673 - -3.1 4,788,673 - -0.5 4,788,690 + -1.5 4,789,061 + Pf1N1B4_4328 0.22 -0.5 4,789,139 + Pf1N1B4_4328 0.29 +0.0 4,789,140 - Pf1N1B4_4328 0.29 -3.1 4,789,268 - Pf1N1B4_4328 0.41 +1.6 4,789,351 + Pf1N1B4_4328 0.48 +1.8 4,789,755 - Pf1N1B4_4328 0.84 -0.2
Or see this region's nucleotide sequence