Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4306 and Pf1N1B4_4307 are separated by 153 nucleotides Pf1N1B4_4307 and Pf1N1B4_4308 are separated by 13 nucleotides Pf1N1B4_4308 and Pf1N1B4_4309 are separated by 273 nucleotides
Pf1N1B4_4306 - Universal stress protein family 5, at 4,768,570 to 4,769,496
_4306
Pf1N1B4_4307 - Threonine dehydrogenase and related Zn-dependent dehydrogenases, at 4,769,650 to 4,770,039
_4307
Pf1N1B4_4308 - Threonine dehydrogenase and related Zn-dependent dehydrogenases, at 4,770,053 to 4,770,202
_4308
Pf1N1B4_4309 - Translation elongation factor Tu, at 4,770,476 to 4,771,393
_4309
Position (kb)
4769
4770
4771 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4768.706 kb on + strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.711 kb on - strand, within Pf1N1B4_4306 at 4768.907 kb on + strand, within Pf1N1B4_4306 at 4768.907 kb on + strand at 4768.908 kb on - strand, within Pf1N1B4_4306 at 4768.913 kb on + strand at 4768.913 kb on + strand, within Pf1N1B4_4306 at 4768.991 kb on + strand, within Pf1N1B4_4306 at 4769.307 kb on - strand, within Pf1N1B4_4306 at 4769.388 kb on + strand, within Pf1N1B4_4306 at 4769.556 kb on - strand at 4769.560 kb on - strand at 4769.763 kb on + strand, within Pf1N1B4_4307 at 4769.763 kb on + strand, within Pf1N1B4_4307 at 4769.763 kb on + strand, within Pf1N1B4_4307 at 4769.764 kb on - strand, within Pf1N1B4_4307 at 4769.764 kb on - strand, within Pf1N1B4_4307 at 4769.764 kb on - strand, within Pf1N1B4_4307 at 4769.800 kb on + strand, within Pf1N1B4_4307 at 4769.931 kb on + strand, within Pf1N1B4_4307 at 4769.932 kb on - strand, within Pf1N1B4_4307 at 4769.951 kb on + strand, within Pf1N1B4_4307 at 4769.952 kb on - strand, within Pf1N1B4_4307 at 4769.952 kb on - strand, within Pf1N1B4_4307 at 4770.370 kb on - strand at 4770.436 kb on - strand at 4770.478 kb on - strand at 4770.729 kb on + strand, within Pf1N1B4_4309 at 4770.755 kb on + strand, within Pf1N1B4_4309 at 4770.799 kb on - strand at 4770.799 kb on - strand, within Pf1N1B4_4309 at 4770.799 kb on - strand, within Pf1N1B4_4309
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 4,768,706 + Pf1N1B4_4306 0.15 -1.5 4,768,707 - Pf1N1B4_4306 0.15 +1.1 4,768,707 - Pf1N1B4_4306 0.15 -1.6 4,768,707 - Pf1N1B4_4306 0.15 -3.1 4,768,707 - Pf1N1B4_4306 0.15 +1.3 4,768,707 - Pf1N1B4_4306 0.15 +0.7 4,768,707 - Pf1N1B4_4306 0.15 +2.1 4,768,707 - Pf1N1B4_4306 0.15 -0.5 4,768,711 - Pf1N1B4_4306 0.15 +0.9 4,768,907 + Pf1N1B4_4306 0.36 +2.1 4,768,907 + -0.5 4,768,908 - Pf1N1B4_4306 0.36 +1.5 4,768,913 + -0.5 4,768,913 + Pf1N1B4_4306 0.37 +0.4 4,768,991 + Pf1N1B4_4306 0.45 +0.5 4,769,307 - Pf1N1B4_4306 0.80 +0.2 4,769,388 + Pf1N1B4_4306 0.88 +0.8 4,769,556 - +2.7 4,769,560 - -0.9 4,769,763 + Pf1N1B4_4307 0.29 +1.1 4,769,763 + Pf1N1B4_4307 0.29 +0.5 4,769,763 + Pf1N1B4_4307 0.29 -3.7 4,769,764 - Pf1N1B4_4307 0.29 -1.5 4,769,764 - Pf1N1B4_4307 0.29 -2.3 4,769,764 - Pf1N1B4_4307 0.29 -0.2 4,769,800 + Pf1N1B4_4307 0.38 +0.5 4,769,931 + Pf1N1B4_4307 0.72 -1.1 4,769,932 - Pf1N1B4_4307 0.72 -1.5 4,769,951 + Pf1N1B4_4307 0.77 +0.5 4,769,952 - Pf1N1B4_4307 0.77 -2.5 4,769,952 - Pf1N1B4_4307 0.77 +1.8 4,770,370 - +0.1 4,770,436 - -0.5 4,770,478 - +2.3 4,770,729 + Pf1N1B4_4309 0.28 +0.3 4,770,755 + Pf1N1B4_4309 0.30 +0.5 4,770,799 - +1.8 4,770,799 - Pf1N1B4_4309 0.35 +0.9 4,770,799 - Pf1N1B4_4309 0.35 +0.2
Or see this region's nucleotide sequence