Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4303 and Pf1N1B4_4304 are separated by 188 nucleotides Pf1N1B4_4304 and Pf1N1B4_4305 are separated by 37 nucleotides Pf1N1B4_4305 and Pf1N1B4_4306 are separated by 145 nucleotides
Pf1N1B4_4303 - DipZ protein, at 4,765,902 to 4,767,113
_4303
Pf1N1B4_4304 - hypothetical protein, at 4,767,302 to 4,767,574
_4304
Pf1N1B4_4305 - PUTATIVE METHYLTRANSFERASE, at 4,767,612 to 4,768,424
_4305
Pf1N1B4_4306 - Universal stress protein family 5, at 4,768,570 to 4,769,496
_4306
Position (kb)
4767
4768
4769 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4766.616 kb on + strand, within Pf1N1B4_4303 at 4766.766 kb on + strand, within Pf1N1B4_4303 at 4766.767 kb on - strand, within Pf1N1B4_4303 at 4766.767 kb on - strand, within Pf1N1B4_4303 at 4766.767 kb on - strand, within Pf1N1B4_4303 at 4766.800 kb on - strand, within Pf1N1B4_4303 at 4766.869 kb on - strand, within Pf1N1B4_4303 at 4766.948 kb on + strand, within Pf1N1B4_4303 at 4766.948 kb on + strand, within Pf1N1B4_4303 at 4766.948 kb on + strand, within Pf1N1B4_4303 at 4766.949 kb on - strand, within Pf1N1B4_4303 at 4766.998 kb on - strand at 4767.033 kb on + strand at 4767.033 kb on + strand at 4767.034 kb on - strand at 4767.034 kb on - strand at 4767.160 kb on + strand at 4767.307 kb on + strand at 4767.308 kb on - strand at 4767.311 kb on + strand at 4767.312 kb on - strand at 4767.312 kb on - strand at 4767.434 kb on + strand, within Pf1N1B4_4304 at 4767.434 kb on + strand, within Pf1N1B4_4304 at 4767.434 kb on + strand, within Pf1N1B4_4304 at 4767.435 kb on - strand at 4767.435 kb on - strand, within Pf1N1B4_4304 at 4767.435 kb on - strand, within Pf1N1B4_4304 at 4767.456 kb on - strand at 4767.462 kb on + strand, within Pf1N1B4_4304 at 4767.464 kb on + strand at 4767.464 kb on + strand, within Pf1N1B4_4304 at 4767.465 kb on - strand at 4767.465 kb on - strand at 4767.618 kb on - strand at 4767.692 kb on + strand at 4767.700 kb on + strand, within Pf1N1B4_4305 at 4767.700 kb on + strand, within Pf1N1B4_4305 at 4767.701 kb on - strand, within Pf1N1B4_4305 at 4767.701 kb on - strand, within Pf1N1B4_4305 at 4767.834 kb on + strand, within Pf1N1B4_4305 at 4767.834 kb on + strand, within Pf1N1B4_4305 at 4768.266 kb on + strand, within Pf1N1B4_4305 at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.592 kb on + strand at 4768.593 kb on - strand at 4768.706 kb on + strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.711 kb on - strand, within Pf1N1B4_4306 at 4768.907 kb on + strand, within Pf1N1B4_4306 at 4768.907 kb on + strand at 4768.908 kb on - strand, within Pf1N1B4_4306 at 4768.913 kb on + strand at 4768.913 kb on + strand, within Pf1N1B4_4306 at 4768.991 kb on + strand, within Pf1N1B4_4306 at 4769.307 kb on - strand, within Pf1N1B4_4306 at 4769.388 kb on + strand, within Pf1N1B4_4306
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 4,766,616 + Pf1N1B4_4303 0.59 -0.5 4,766,766 + Pf1N1B4_4303 0.71 -1.1 4,766,767 - Pf1N1B4_4303 0.71 -1.1 4,766,767 - Pf1N1B4_4303 0.71 +0.8 4,766,767 - Pf1N1B4_4303 0.71 -2.1 4,766,800 - Pf1N1B4_4303 0.74 +0.9 4,766,869 - Pf1N1B4_4303 0.80 +1.5 4,766,948 + Pf1N1B4_4303 0.86 -1.3 4,766,948 + Pf1N1B4_4303 0.86 -1.5 4,766,948 + Pf1N1B4_4303 0.86 -0.5 4,766,949 - Pf1N1B4_4303 0.86 +3.7 4,766,998 - -1.2 4,767,033 + +0.1 4,767,033 + +0.5 4,767,034 - -3.5 4,767,034 - -0.9 4,767,160 + -0.6 4,767,307 + -3.3 4,767,308 - +1.1 4,767,311 + -0.5 4,767,312 - -0.5 4,767,312 - -0.5 4,767,434 + Pf1N1B4_4304 0.48 -1.5 4,767,434 + Pf1N1B4_4304 0.48 +1.8 4,767,434 + Pf1N1B4_4304 0.48 -0.5 4,767,435 - -1.5 4,767,435 - Pf1N1B4_4304 0.49 +0.1 4,767,435 - Pf1N1B4_4304 0.49 +2.0 4,767,456 - -0.5 4,767,462 + Pf1N1B4_4304 0.59 +0.8 4,767,464 + -1.5 4,767,464 + Pf1N1B4_4304 0.59 +0.5 4,767,465 - +1.5 4,767,465 - +0.1 4,767,618 - +0.9 4,767,692 + +1.5 4,767,700 + Pf1N1B4_4305 0.11 +0.2 4,767,700 + Pf1N1B4_4305 0.11 -0.5 4,767,701 - Pf1N1B4_4305 0.11 -1.1 4,767,701 - Pf1N1B4_4305 0.11 +0.5 4,767,834 + Pf1N1B4_4305 0.27 -0.9 4,767,834 + Pf1N1B4_4305 0.27 -0.9 4,768,266 + Pf1N1B4_4305 0.80 -1.5 4,768,435 + -0.5 4,768,435 + -0.1 4,768,435 + -2.5 4,768,435 + -0.1 4,768,435 + -0.5 4,768,435 + -2.1 4,768,435 + -1.5 4,768,435 + -1.8 4,768,435 + -1.1 4,768,436 - +0.9 4,768,436 - -0.9 4,768,436 - -1.5 4,768,436 - -0.9 4,768,436 - -0.5 4,768,436 - -1.5 4,768,436 - +0.3 4,768,436 - +1.1 4,768,436 - -1.2 4,768,436 - +0.1 4,768,436 - -1.7 4,768,436 - -0.5 4,768,436 - -4.0 4,768,592 + -2.1 4,768,593 - +1.3 4,768,706 + Pf1N1B4_4306 0.15 -1.5 4,768,707 - Pf1N1B4_4306 0.15 +1.1 4,768,707 - Pf1N1B4_4306 0.15 -1.6 4,768,707 - Pf1N1B4_4306 0.15 -3.1 4,768,707 - Pf1N1B4_4306 0.15 +1.3 4,768,707 - Pf1N1B4_4306 0.15 +0.7 4,768,707 - Pf1N1B4_4306 0.15 +2.1 4,768,707 - Pf1N1B4_4306 0.15 -0.5 4,768,711 - Pf1N1B4_4306 0.15 +0.9 4,768,907 + Pf1N1B4_4306 0.36 +2.1 4,768,907 + -0.5 4,768,908 - Pf1N1B4_4306 0.36 +1.5 4,768,913 + -0.5 4,768,913 + Pf1N1B4_4306 0.37 +0.4 4,768,991 + Pf1N1B4_4306 0.45 +0.5 4,769,307 - Pf1N1B4_4306 0.80 +0.2 4,769,388 + Pf1N1B4_4306 0.88 +0.8
Or see this region's nucleotide sequence