Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4280 and Pf1N1B4_4281 are separated by 153 nucleotides Pf1N1B4_4281 and Pf1N1B4_4282 are separated by 134 nucleotides
Pf1N1B4_4280 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 4,746,718 to 4,747,743
_4280
Pf1N1B4_4281 - Glyceraldehyde-3-phosphate ketol-isomerase (EC 5.3.1.1), at 4,747,897 to 4,748,757
_4281
Pf1N1B4_4282 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 4,748,892 to 4,749,944
_4282
Position (kb)
4747
4748
4749 Strain fitness (log2 ratio)
-2
-1
0
1 at 4747.211 kb on + strand, within Pf1N1B4_4280 at 4747.222 kb on + strand, within Pf1N1B4_4280 at 4747.297 kb on + strand, within Pf1N1B4_4280 at 4747.297 kb on + strand, within Pf1N1B4_4280 at 4747.297 kb on + strand, within Pf1N1B4_4280 at 4747.297 kb on + strand, within Pf1N1B4_4280 at 4747.298 kb on - strand, within Pf1N1B4_4280 at 4747.298 kb on - strand, within Pf1N1B4_4280 at 4747.298 kb on - strand, within Pf1N1B4_4280 at 4747.703 kb on + strand at 4747.736 kb on - strand at 4747.919 kb on - strand at 4747.924 kb on + strand at 4747.925 kb on - strand at 4748.020 kb on + strand, within Pf1N1B4_4281 at 4748.020 kb on + strand, within Pf1N1B4_4281 at 4748.021 kb on - strand, within Pf1N1B4_4281 at 4748.021 kb on - strand, within Pf1N1B4_4281 at 4748.021 kb on - strand, within Pf1N1B4_4281 at 4748.102 kb on + strand, within Pf1N1B4_4281 at 4748.103 kb on - strand, within Pf1N1B4_4281 at 4748.113 kb on + strand, within Pf1N1B4_4281 at 4748.114 kb on - strand, within Pf1N1B4_4281 at 4748.114 kb on - strand, within Pf1N1B4_4281 at 4748.114 kb on - strand, within Pf1N1B4_4281 at 4748.339 kb on - strand, within Pf1N1B4_4281 at 4748.408 kb on - strand, within Pf1N1B4_4281 at 4748.437 kb on + strand, within Pf1N1B4_4281 at 4748.477 kb on - strand, within Pf1N1B4_4281 at 4748.477 kb on - strand, within Pf1N1B4_4281 at 4748.518 kb on + strand, within Pf1N1B4_4281 at 4748.518 kb on + strand, within Pf1N1B4_4281 at 4748.518 kb on + strand, within Pf1N1B4_4281 at 4748.519 kb on - strand, within Pf1N1B4_4281 at 4748.875 kb on + strand at 4748.875 kb on + strand at 4748.875 kb on + strand at 4748.875 kb on + strand at 4748.875 kb on + strand at 4749.457 kb on - strand, within Pf1N1B4_4282 at 4749.471 kb on + strand, within Pf1N1B4_4282 at 4749.509 kb on + strand, within Pf1N1B4_4282 at 4749.510 kb on - strand at 4749.510 kb on - strand, within Pf1N1B4_4282 at 4749.510 kb on - strand, within Pf1N1B4_4282 at 4749.613 kb on - strand, within Pf1N1B4_4282
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 4,747,211 + Pf1N1B4_4280 0.48 -2.5 4,747,222 + Pf1N1B4_4280 0.49 -1.1 4,747,297 + Pf1N1B4_4280 0.56 -2.1 4,747,297 + Pf1N1B4_4280 0.56 +0.8 4,747,297 + Pf1N1B4_4280 0.56 +1.1 4,747,297 + Pf1N1B4_4280 0.56 -0.5 4,747,298 - Pf1N1B4_4280 0.57 +1.6 4,747,298 - Pf1N1B4_4280 0.57 -1.1 4,747,298 - Pf1N1B4_4280 0.57 +0.7 4,747,703 + -1.0 4,747,736 - +0.9 4,747,919 - -1.5 4,747,924 + +0.6 4,747,925 - +0.5 4,748,020 + Pf1N1B4_4281 0.14 +0.8 4,748,020 + Pf1N1B4_4281 0.14 +0.1 4,748,021 - Pf1N1B4_4281 0.14 -0.2 4,748,021 - Pf1N1B4_4281 0.14 -0.5 4,748,021 - Pf1N1B4_4281 0.14 -0.5 4,748,102 + Pf1N1B4_4281 0.24 -0.1 4,748,103 - Pf1N1B4_4281 0.24 -0.5 4,748,113 + Pf1N1B4_4281 0.25 -2.5 4,748,114 - Pf1N1B4_4281 0.25 -1.9 4,748,114 - Pf1N1B4_4281 0.25 -0.5 4,748,114 - Pf1N1B4_4281 0.25 -0.5 4,748,339 - Pf1N1B4_4281 0.51 -0.1 4,748,408 - Pf1N1B4_4281 0.59 -0.0 4,748,437 + Pf1N1B4_4281 0.63 -0.5 4,748,477 - Pf1N1B4_4281 0.67 +1.1 4,748,477 - Pf1N1B4_4281 0.67 -0.2 4,748,518 + Pf1N1B4_4281 0.72 -0.2 4,748,518 + Pf1N1B4_4281 0.72 -1.3 4,748,518 + Pf1N1B4_4281 0.72 +1.3 4,748,519 - Pf1N1B4_4281 0.72 +0.8 4,748,875 + -0.0 4,748,875 + -0.2 4,748,875 + +0.2 4,748,875 + +0.2 4,748,875 + +0.6 4,749,457 - Pf1N1B4_4282 0.54 +1.0 4,749,471 + Pf1N1B4_4282 0.55 -0.9 4,749,509 + Pf1N1B4_4282 0.59 -0.2 4,749,510 - -0.5 4,749,510 - Pf1N1B4_4282 0.59 -0.1 4,749,510 - Pf1N1B4_4282 0.59 -0.7 4,749,613 - Pf1N1B4_4282 0.68 -2.5
Or see this region's nucleotide sequence