Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4109 and Pf1N1B4_4110 are separated by 130 nucleotides Pf1N1B4_4110 and Pf1N1B4_4111 are separated by 173 nucleotides Pf1N1B4_4111 and Pf1N1B4_4112 are separated by 141 nucleotides Pf1N1B4_4112 and Pf1N1B4_4113 overlap by 4 nucleotides Pf1N1B4_4113 and Pf1N1B4_4114 overlap by 2 nucleotides
Pf1N1B4_4109 - Cytochrome c2, at 4,555,852 to 4,556,241
_4109
Pf1N1B4_4110 - hypothetical protein, at 4,556,372 to 4,556,707
_4110
Pf1N1B4_4111 - Panthothenate synthetase, at 4,556,881 to 4,557,171
_4111
Pf1N1B4_4112 - hypothetical protein, at 4,557,313 to 4,557,564
_4112
Pf1N1B4_4113 - Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1), at 4,557,561 to 4,558,040
_4113
Pf1N1B4_4114 - hypothetical protein, at 4,558,039 to 4,558,152
_4114
Position (kb)
4556
4557
4558 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4555.952 kb on + strand, within Pf1N1B4_4109 at 4555.952 kb on + strand, within Pf1N1B4_4109 at 4555.953 kb on - strand, within Pf1N1B4_4109 at 4555.953 kb on - strand at 4555.953 kb on - strand at 4555.962 kb on + strand, within Pf1N1B4_4109 at 4555.963 kb on - strand, within Pf1N1B4_4109 at 4555.985 kb on + strand, within Pf1N1B4_4109 at 4555.986 kb on - strand, within Pf1N1B4_4109 at 4556.062 kb on + strand at 4556.063 kb on - strand, within Pf1N1B4_4109 at 4556.068 kb on + strand, within Pf1N1B4_4109 at 4556.119 kb on - strand, within Pf1N1B4_4109 at 4556.125 kb on + strand, within Pf1N1B4_4109 at 4556.147 kb on + strand, within Pf1N1B4_4109 at 4556.216 kb on - strand at 4556.251 kb on + strand at 4556.378 kb on + strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.474 kb on + strand, within Pf1N1B4_4110 at 4556.474 kb on + strand, within Pf1N1B4_4110 at 4556.475 kb on - strand, within Pf1N1B4_4110 at 4556.475 kb on - strand, within Pf1N1B4_4110 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.188 kb on - strand at 4557.194 kb on + strand at 4557.231 kb on + strand at 4557.231 kb on + strand at 4557.231 kb on + strand at 4557.232 kb on - strand at 4557.291 kb on - strand at 4557.328 kb on + strand at 4557.329 kb on - strand at 4557.423 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.425 kb on + strand, within Pf1N1B4_4112 at 4557.426 kb on - strand, within Pf1N1B4_4112 at 4557.426 kb on - strand, within Pf1N1B4_4112 at 4557.426 kb on - strand, within Pf1N1B4_4112 at 4557.426 kb on - strand, within Pf1N1B4_4112 at 4557.426 kb on - strand, within Pf1N1B4_4112 at 4557.472 kb on - strand, within Pf1N1B4_4112 at 4557.472 kb on - strand, within Pf1N1B4_4112 at 4557.472 kb on - strand, within Pf1N1B4_4112 at 4557.549 kb on - strand at 4557.971 kb on + strand at 4557.972 kb on - strand, within Pf1N1B4_4113 at 4557.972 kb on - strand, within Pf1N1B4_4113 at 4558.062 kb on - strand, within Pf1N1B4_4114 at 4558.062 kb on - strand, within Pf1N1B4_4114
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 4,555,952 + Pf1N1B4_4109 0.26 +0.1 4,555,952 + Pf1N1B4_4109 0.26 +0.1 4,555,953 - Pf1N1B4_4109 0.26 +1.1 4,555,953 - -0.5 4,555,953 - +1.1 4,555,962 + Pf1N1B4_4109 0.28 +1.1 4,555,963 - Pf1N1B4_4109 0.28 -1.8 4,555,985 + Pf1N1B4_4109 0.34 +1.0 4,555,986 - Pf1N1B4_4109 0.34 -1.5 4,556,062 + +0.5 4,556,063 - Pf1N1B4_4109 0.54 -2.8 4,556,068 + Pf1N1B4_4109 0.55 -1.5 4,556,119 - Pf1N1B4_4109 0.68 -2.1 4,556,125 + Pf1N1B4_4109 0.70 -0.5 4,556,147 + Pf1N1B4_4109 0.76 +0.7 4,556,216 - -0.5 4,556,251 + -0.1 4,556,378 + -0.1 4,556,379 - -0.5 4,556,379 - -2.1 4,556,379 - +1.1 4,556,379 - +0.8 4,556,379 - -0.3 4,556,474 + Pf1N1B4_4110 0.30 -1.1 4,556,474 + Pf1N1B4_4110 0.30 -1.5 4,556,475 - Pf1N1B4_4110 0.31 +0.3 4,556,475 - Pf1N1B4_4110 0.31 +0.7 4,557,020 - Pf1N1B4_4111 0.48 -0.5 4,557,020 - Pf1N1B4_4111 0.48 -2.2 4,557,020 - Pf1N1B4_4111 0.48 -1.5 4,557,020 - Pf1N1B4_4111 0.48 -0.8 4,557,020 - Pf1N1B4_4111 0.48 -2.3 4,557,188 - -0.5 4,557,194 + -2.5 4,557,231 + -1.5 4,557,231 + +0.1 4,557,231 + +0.1 4,557,232 - -2.5 4,557,291 - +1.5 4,557,328 + -2.5 4,557,329 - -2.5 4,557,423 + Pf1N1B4_4112 0.44 -1.5 4,557,425 + Pf1N1B4_4112 0.44 -0.0 4,557,425 + +1.8 4,557,425 + Pf1N1B4_4112 0.44 -2.5 4,557,425 + Pf1N1B4_4112 0.44 -2.8 4,557,425 + Pf1N1B4_4112 0.44 -3.5 4,557,425 + Pf1N1B4_4112 0.44 +2.5 4,557,425 + Pf1N1B4_4112 0.44 +1.5 4,557,425 + Pf1N1B4_4112 0.44 -0.8 4,557,425 + Pf1N1B4_4112 0.44 +0.3 4,557,426 - Pf1N1B4_4112 0.45 -1.5 4,557,426 - Pf1N1B4_4112 0.45 +0.1 4,557,426 - Pf1N1B4_4112 0.45 -0.0 4,557,426 - Pf1N1B4_4112 0.45 +0.0 4,557,426 - Pf1N1B4_4112 0.45 -1.4 4,557,472 - Pf1N1B4_4112 0.63 -2.5 4,557,472 - Pf1N1B4_4112 0.63 -2.5 4,557,472 - Pf1N1B4_4112 0.63 -2.8 4,557,549 - -2.1 4,557,971 + -0.5 4,557,972 - Pf1N1B4_4113 0.86 -1.5 4,557,972 - Pf1N1B4_4113 0.86 -1.5 4,558,062 - Pf1N1B4_4114 0.20 -1.5 4,558,062 - Pf1N1B4_4114 0.20 -0.5
Or see this region's nucleotide sequence