Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4041 and Pf1N1B4_4042 are separated by 649 nucleotides Pf1N1B4_4042 and Pf1N1B4_4043 are separated by 498 nucleotides
Pf1N1B4_4041 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 4,492,779 to 4,493,192
_4041
Pf1N1B4_4042 - Isocitrate lyase (EC 4.1.3.1) (from data) , at 4,493,842 to 4,495,167
_4042
Pf1N1B4_4043 - Type II and III secretion system family protein, at 4,495,666 to 4,496,427
_4043
Position (kb)
4493
4494
4495
4496 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.879 kb on - strand, within Pf1N1B4_4041 at 4492.994 kb on - strand, within Pf1N1B4_4041 at 4492.994 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.284 kb on + strand at 4493.326 kb on - strand at 4493.329 kb on + strand at 4493.330 kb on - strand at 4493.345 kb on + strand at 4493.406 kb on + strand at 4493.406 kb on + strand at 4493.406 kb on + strand at 4493.425 kb on - strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.551 kb on + strand at 4493.780 kb on + strand at 4493.781 kb on - strand at 4493.781 kb on - strand at 4493.825 kb on + strand at 4493.930 kb on + strand at 4494.111 kb on - strand, within Pf1N1B4_4042 at 4494.198 kb on - strand, within Pf1N1B4_4042 at 4494.198 kb on - strand, within Pf1N1B4_4042 at 4494.308 kb on + strand, within Pf1N1B4_4042 at 4494.356 kb on + strand, within Pf1N1B4_4042 at 4494.357 kb on - strand, within Pf1N1B4_4042 at 4494.402 kb on - strand, within Pf1N1B4_4042 at 4494.705 kb on - strand, within Pf1N1B4_4042 at 4494.705 kb on - strand, within Pf1N1B4_4042 at 4494.859 kb on + strand, within Pf1N1B4_4042 at 4494.860 kb on - strand, within Pf1N1B4_4042 at 4494.860 kb on - strand, within Pf1N1B4_4042 at 4494.878 kb on + strand, within Pf1N1B4_4042 at 4494.878 kb on + strand, within Pf1N1B4_4042 at 4494.879 kb on - strand, within Pf1N1B4_4042 at 4495.083 kb on - strand at 4495.187 kb on + strand at 4495.188 kb on - strand at 4495.188 kb on - strand at 4495.267 kb on + strand at 4495.267 kb on + strand at 4495.267 kb on + strand at 4495.268 kb on - strand at 4495.268 kb on - strand at 4495.268 kb on - strand at 4495.268 kb on - strand at 4495.337 kb on + strand at 4495.409 kb on + strand at 4495.409 kb on + strand at 4495.410 kb on - strand at 4495.414 kb on - strand at 4495.623 kb on - strand at 4495.635 kb on + strand at 4495.636 kb on - strand at 4495.638 kb on + strand at 4495.638 kb on + strand at 4495.638 kb on + strand at 4495.668 kb on - strand at 4495.678 kb on - strand at 4495.724 kb on + strand at 4495.724 kb on + strand at 4495.724 kb on + strand at 4495.725 kb on - strand at 4495.837 kb on + strand, within Pf1N1B4_4043 at 4495.837 kb on + strand at 4495.838 kb on - strand, within Pf1N1B4_4043 at 4495.875 kb on + strand, within Pf1N1B4_4043 at 4495.922 kb on + strand, within Pf1N1B4_4043 at 4495.974 kb on - strand, within Pf1N1B4_4043 at 4496.154 kb on + strand, within Pf1N1B4_4043 at 4496.155 kb on - strand, within Pf1N1B4_4043
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 4,492,853 - Pf1N1B4_4041 0.18 +1.1 4,492,853 - Pf1N1B4_4041 0.18 +1.0 4,492,853 - Pf1N1B4_4041 0.18 +2.8 4,492,879 - Pf1N1B4_4041 0.24 +1.5 4,492,994 - Pf1N1B4_4041 0.52 -1.0 4,492,994 - Pf1N1B4_4041 0.52 -0.4 4,493,035 - Pf1N1B4_4041 0.62 +0.6 4,493,035 - Pf1N1B4_4041 0.62 +1.1 4,493,035 - Pf1N1B4_4041 0.62 -0.4 4,493,284 + -0.4 4,493,326 - -0.4 4,493,329 + +0.0 4,493,330 - +0.1 4,493,345 + +0.1 4,493,406 + -2.4 4,493,406 + +2.9 4,493,406 + -0.8 4,493,425 - +1.6 4,493,433 + +1.1 4,493,433 + -0.0 4,493,433 + -0.2 4,493,433 + -2.0 4,493,433 + -0.4 4,493,433 + +0.3 4,493,433 + +0.8 4,493,433 + -1.2 4,493,433 + -0.4 4,493,433 + +0.8 4,493,433 + -2.4 4,493,434 - -1.4 4,493,434 - -0.4 4,493,434 - +0.8 4,493,434 - -1.4 4,493,434 - +1.1 4,493,513 + -1.0 4,493,513 + -2.8 4,493,513 + -2.0 4,493,513 + -2.0 4,493,514 - -0.3 4,493,514 - -2.8 4,493,514 - +0.8 4,493,514 - +1.6 4,493,514 - +1.5 4,493,515 + +0.4 4,493,515 + -2.8 4,493,515 + +2.0 4,493,515 + -0.4 4,493,515 + -3.4 4,493,516 - -0.2 4,493,516 - +1.1 4,493,516 - -2.4 4,493,516 - +1.1 4,493,551 + -0.4 4,493,780 + -1.2 4,493,781 - +0.4 4,493,781 - -0.0 4,493,825 + -1.2 4,493,930 + -2.0 4,494,111 - Pf1N1B4_4042 0.20 -0.4 4,494,198 - Pf1N1B4_4042 0.27 +0.9 4,494,198 - Pf1N1B4_4042 0.27 +0.6 4,494,308 + Pf1N1B4_4042 0.35 -2.4 4,494,356 + Pf1N1B4_4042 0.39 -1.8 4,494,357 - Pf1N1B4_4042 0.39 -0.8 4,494,402 - Pf1N1B4_4042 0.42 -1.2 4,494,705 - Pf1N1B4_4042 0.65 -0.9 4,494,705 - Pf1N1B4_4042 0.65 -2.8 4,494,859 + Pf1N1B4_4042 0.77 -0.4 4,494,860 - Pf1N1B4_4042 0.77 +1.4 4,494,860 - Pf1N1B4_4042 0.77 -2.0 4,494,878 + Pf1N1B4_4042 0.78 -1.4 4,494,878 + Pf1N1B4_4042 0.78 +0.1 4,494,879 - Pf1N1B4_4042 0.78 -2.4 4,495,083 - -1.4 4,495,187 + -0.4 4,495,188 - +1.1 4,495,188 - -1.1 4,495,267 + -2.8 4,495,267 + -0.4 4,495,267 + -0.0 4,495,268 - +1.0 4,495,268 - -1.8 4,495,268 - -2.6 4,495,268 - -2.6 4,495,337 + -0.5 4,495,409 + +0.2 4,495,409 + -0.5 4,495,410 - +1.1 4,495,414 - -3.0 4,495,623 - +1.9 4,495,635 + +1.5 4,495,636 - -1.5 4,495,638 + -2.5 4,495,638 + +0.0 4,495,638 + -1.5 4,495,668 - -2.5 4,495,678 - -1.7 4,495,724 + -0.0 4,495,724 + -0.5 4,495,724 + -1.5 4,495,725 - +0.3 4,495,837 + Pf1N1B4_4043 0.22 +0.1 4,495,837 + +0.9 4,495,838 - Pf1N1B4_4043 0.23 -0.5 4,495,875 + Pf1N1B4_4043 0.27 +0.1 4,495,922 + Pf1N1B4_4043 0.34 -0.0 4,495,974 - Pf1N1B4_4043 0.40 -1.0 4,496,154 + Pf1N1B4_4043 0.64 -0.5 4,496,155 - Pf1N1B4_4043 0.64 +1.4
Or see this region's nucleotide sequence