Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_386 and Pf1N1B4_387 overlap by 24 nucleotides Pf1N1B4_387 and Pf1N1B4_388 are separated by 120 nucleotides Pf1N1B4_388 and Pf1N1B4_389 overlap by 4 nucleotides
Pf1N1B4_386 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 455,166 to 456,605
_386
Pf1N1B4_387 - hypothetical protein, at 456,582 to 456,695
_387
Pf1N1B4_388 - OmpA family protein, at 456,816 to 457,628
_388
Pf1N1B4_389 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 457,625 to 457,981
_389
Position (kb)
456
457 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 455.611 kb on + strand, within Pf1N1B4_386 at 455.611 kb on + strand at 455.611 kb on + strand, within Pf1N1B4_386 at 455.611 kb on + strand, within Pf1N1B4_386 at 455.611 kb on + strand, within Pf1N1B4_386 at 455.612 kb on - strand, within Pf1N1B4_386 at 455.612 kb on - strand, within Pf1N1B4_386 at 455.612 kb on - strand, within Pf1N1B4_386 at 455.612 kb on - strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.677 kb on + strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.678 kb on - strand, within Pf1N1B4_386 at 455.701 kb on + strand, within Pf1N1B4_386 at 455.750 kb on - strand, within Pf1N1B4_386 at 455.750 kb on - strand, within Pf1N1B4_386 at 455.753 kb on + strand, within Pf1N1B4_386 at 455.754 kb on - strand, within Pf1N1B4_386 at 455.754 kb on - strand, within Pf1N1B4_386 at 455.754 kb on - strand, within Pf1N1B4_386 at 455.844 kb on - strand, within Pf1N1B4_386 at 455.844 kb on - strand, within Pf1N1B4_386 at 455.989 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 456.036 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.245 kb on - strand, within Pf1N1B4_386 at 456.245 kb on - strand, within Pf1N1B4_386 at 456.245 kb on - strand, within Pf1N1B4_386 at 456.245 kb on - strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.404 kb on - strand, within Pf1N1B4_386 at 456.404 kb on - strand, within Pf1N1B4_386 at 456.538 kb on + strand at 456.538 kb on + strand at 456.539 kb on - strand at 456.539 kb on - strand at 456.578 kb on + strand at 456.578 kb on + strand at 456.578 kb on + strand at 456.578 kb on + strand at 456.579 kb on - strand at 456.579 kb on - strand at 456.579 kb on - strand at 456.579 kb on - strand at 456.586 kb on + strand at 456.586 kb on + strand at 456.586 kb on + strand at 456.586 kb on + strand at 456.634 kb on + strand, within Pf1N1B4_387 at 456.634 kb on + strand, within Pf1N1B4_387 at 456.634 kb on + strand, within Pf1N1B4_387 at 456.635 kb on - strand, within Pf1N1B4_387 at 456.665 kb on + strand, within Pf1N1B4_387 at 456.673 kb on + strand, within Pf1N1B4_387 at 456.703 kb on + strand at 456.703 kb on + strand at 456.703 kb on + strand at 456.703 kb on + strand at 456.944 kb on + strand at 456.944 kb on + strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 457.048 kb on + strand, within Pf1N1B4_388 at 457.049 kb on - strand, within Pf1N1B4_388 at 457.049 kb on - strand, within Pf1N1B4_388 at 457.366 kb on + strand, within Pf1N1B4_388 at 457.367 kb on - strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.382 kb on - strand, within Pf1N1B4_388 at 457.387 kb on + strand, within Pf1N1B4_388 at 457.388 kb on - strand, within Pf1N1B4_388 at 457.388 kb on - strand, within Pf1N1B4_388 at 457.418 kb on - strand, within Pf1N1B4_388 at 457.418 kb on - strand, within Pf1N1B4_388 at 457.571 kb on - strand at 457.670 kb on - strand, within Pf1N1B4_389
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 455,611 + Pf1N1B4_386 0.31 -0.1 455,611 + +0.7 455,611 + Pf1N1B4_386 0.31 +1.3 455,611 + Pf1N1B4_386 0.31 -0.1 455,611 + Pf1N1B4_386 0.31 +0.7 455,612 - Pf1N1B4_386 0.31 -1.1 455,612 - Pf1N1B4_386 0.31 -1.1 455,612 - Pf1N1B4_386 0.31 -0.1 455,612 - Pf1N1B4_386 0.31 +2.4 455,677 + Pf1N1B4_386 0.35 -1.1 455,677 + Pf1N1B4_386 0.35 -1.1 455,677 + Pf1N1B4_386 0.35 -1.7 455,677 + Pf1N1B4_386 0.35 -0.9 455,677 + Pf1N1B4_386 0.35 +0.5 455,677 + Pf1N1B4_386 0.35 -1.1 455,677 + Pf1N1B4_386 0.35 -1.5 455,677 + Pf1N1B4_386 0.35 -2.1 455,677 + Pf1N1B4_386 0.35 +0.9 455,678 - Pf1N1B4_386 0.36 -0.5 455,678 - Pf1N1B4_386 0.36 -1.7 455,678 - Pf1N1B4_386 0.36 -1.1 455,678 - Pf1N1B4_386 0.36 -2.4 455,678 - Pf1N1B4_386 0.36 -0.5 455,678 - Pf1N1B4_386 0.36 +1.3 455,678 - Pf1N1B4_386 0.36 +1.9 455,678 - Pf1N1B4_386 0.36 +0.9 455,701 + Pf1N1B4_386 0.37 -1.7 455,750 - Pf1N1B4_386 0.41 +0.6 455,750 - Pf1N1B4_386 0.41 -0.1 455,753 + Pf1N1B4_386 0.41 +0.6 455,754 - Pf1N1B4_386 0.41 -2.1 455,754 - Pf1N1B4_386 0.41 -0.7 455,754 - Pf1N1B4_386 0.41 +0.3 455,844 - Pf1N1B4_386 0.47 -0.1 455,844 - Pf1N1B4_386 0.47 -0.7 455,989 + Pf1N1B4_386 0.57 +1.9 455,992 + Pf1N1B4_386 0.57 +0.4 455,992 + Pf1N1B4_386 0.57 +2.7 455,992 + Pf1N1B4_386 0.57 -1.1 455,992 + -1.1 455,992 + Pf1N1B4_386 0.57 -2.7 455,992 + Pf1N1B4_386 0.57 -1.4 455,992 + Pf1N1B4_386 0.57 -0.7 455,992 + Pf1N1B4_386 0.57 -1.4 455,992 + Pf1N1B4_386 0.57 +0.3 455,992 + Pf1N1B4_386 0.57 +0.1 456,036 + Pf1N1B4_386 0.60 -1.7 456,172 + Pf1N1B4_386 0.70 -1.4 456,172 + Pf1N1B4_386 0.70 -2.4 456,172 + Pf1N1B4_386 0.70 -0.1 456,172 + -0.1 456,172 + Pf1N1B4_386 0.70 +0.5 456,172 + Pf1N1B4_386 0.70 -2.1 456,172 + Pf1N1B4_386 0.70 -0.7 456,173 - Pf1N1B4_386 0.70 -0.3 456,173 - Pf1N1B4_386 0.70 -0.1 456,173 - Pf1N1B4_386 0.70 -0.1 456,173 - Pf1N1B4_386 0.70 +1.5 456,173 - Pf1N1B4_386 0.70 -0.5 456,245 - Pf1N1B4_386 0.75 -0.7 456,245 - Pf1N1B4_386 0.75 -0.1 456,245 - Pf1N1B4_386 0.75 -1.1 456,245 - Pf1N1B4_386 0.75 -1.7 456,403 + Pf1N1B4_386 0.86 -1.1 456,403 + Pf1N1B4_386 0.86 -1.7 456,403 + Pf1N1B4_386 0.86 -0.5 456,403 + Pf1N1B4_386 0.86 -0.5 456,403 + Pf1N1B4_386 0.86 +0.3 456,403 + Pf1N1B4_386 0.86 +0.1 456,404 - Pf1N1B4_386 0.86 -1.1 456,404 - Pf1N1B4_386 0.86 +0.5 456,538 + -1.1 456,538 + +0.6 456,539 - -1.7 456,539 - +0.4 456,578 + -0.1 456,578 + -0.8 456,578 + -2.9 456,578 + -0.3 456,579 - +1.3 456,579 - -1.1 456,579 - +2.3 456,579 - -1.1 456,586 + -0.1 456,586 + +0.5 456,586 + -0.3 456,586 + -0.1 456,634 + Pf1N1B4_387 0.46 -0.1 456,634 + Pf1N1B4_387 0.46 -0.1 456,634 + Pf1N1B4_387 0.46 -1.1 456,635 - Pf1N1B4_387 0.46 -0.1 456,665 + Pf1N1B4_387 0.73 -2.4 456,673 + Pf1N1B4_387 0.80 -1.7 456,703 + -2.7 456,703 + +0.2 456,703 + -1.1 456,703 + +1.3 456,944 + -1.1 456,944 + Pf1N1B4_388 0.16 -1.1 456,945 - Pf1N1B4_388 0.16 -1.7 456,945 - Pf1N1B4_388 0.16 -0.7 456,945 - Pf1N1B4_388 0.16 -2.4 456,945 - Pf1N1B4_388 0.16 -2.8 456,945 - -0.1 456,945 - Pf1N1B4_388 0.16 -2.4 456,945 - Pf1N1B4_388 0.16 -3.4 456,945 - Pf1N1B4_388 0.16 -1.1 457,048 + Pf1N1B4_388 0.29 -0.7 457,049 - Pf1N1B4_388 0.29 -1.5 457,049 - Pf1N1B4_388 0.29 -1.3 457,366 + Pf1N1B4_388 0.68 -0.7 457,367 - Pf1N1B4_388 0.68 -2.4 457,381 + Pf1N1B4_388 0.69 -1.1 457,381 + Pf1N1B4_388 0.69 -1.1 457,381 + Pf1N1B4_388 0.69 -0.1 457,381 + Pf1N1B4_388 0.69 +0.7 457,381 + Pf1N1B4_388 0.69 -2.1 457,382 - Pf1N1B4_388 0.70 -1.7 457,387 + Pf1N1B4_388 0.70 -1.1 457,388 - Pf1N1B4_388 0.70 -0.8 457,388 - Pf1N1B4_388 0.70 -2.4 457,418 - Pf1N1B4_388 0.74 -2.4 457,418 - Pf1N1B4_388 0.74 -2.4 457,571 - +0.5 457,670 - Pf1N1B4_389 0.13 -2.4
Or see this region's nucleotide sequence