Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3627 and Pf1N1B4_3628 are separated by 30 nucleotides Pf1N1B4_3628 and Pf1N1B4_3629 are separated by 55 nucleotides Pf1N1B4_3629 and Pf1N1B4_3630 are separated by 49 nucleotides
Pf1N1B4_3627 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 4,035,177 to 4,035,560
_3627
Pf1N1B4_3628 - Chemotaxis response - phosphatase CheZ, at 4,035,591 to 4,036,379
_3628
Pf1N1B4_3629 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 4,036,435 to 4,038,678
_3629
Pf1N1B4_3630 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 4,038,728 to 4,039,861
_3630
Position (kb)
4036
4037
4038
4039 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4035.489 kb on + strand, within Pf1N1B4_3627 at 4035.489 kb on + strand, within Pf1N1B4_3627 at 4035.489 kb on + strand, within Pf1N1B4_3627 at 4035.489 kb on + strand, within Pf1N1B4_3627 at 4035.591 kb on - strand at 4035.591 kb on - strand at 4035.739 kb on - strand, within Pf1N1B4_3628 at 4035.861 kb on + strand, within Pf1N1B4_3628 at 4035.862 kb on - strand, within Pf1N1B4_3628 at 4036.378 kb on - strand at 4036.385 kb on + strand at 4036.386 kb on - strand at 4036.410 kb on + strand at 4036.411 kb on - strand at 4036.411 kb on - strand at 4036.434 kb on - strand at 4036.586 kb on + strand at 4036.587 kb on - strand at 4036.587 kb on - strand at 4037.075 kb on + strand, within Pf1N1B4_3629 at 4037.075 kb on + strand, within Pf1N1B4_3629 at 4037.133 kb on + strand, within Pf1N1B4_3629 at 4037.181 kb on + strand, within Pf1N1B4_3629 at 4037.576 kb on + strand, within Pf1N1B4_3629 at 4037.576 kb on + strand, within Pf1N1B4_3629 at 4038.201 kb on + strand, within Pf1N1B4_3629 at 4038.201 kb on + strand, within Pf1N1B4_3629 at 4038.201 kb on + strand, within Pf1N1B4_3629 at 4038.202 kb on - strand at 4038.205 kb on + strand, within Pf1N1B4_3629 at 4038.205 kb on + strand, within Pf1N1B4_3629 at 4038.206 kb on - strand, within Pf1N1B4_3629 at 4038.206 kb on - strand, within Pf1N1B4_3629 at 4038.206 kb on - strand, within Pf1N1B4_3629 at 4038.206 kb on - strand, within Pf1N1B4_3629 at 4038.415 kb on + strand, within Pf1N1B4_3629 at 4038.415 kb on + strand, within Pf1N1B4_3629 at 4038.416 kb on - strand, within Pf1N1B4_3629 at 4038.672 kb on + strand at 4039.067 kb on - strand, within Pf1N1B4_3630 at 4039.111 kb on + strand, within Pf1N1B4_3630 at 4039.142 kb on + strand, within Pf1N1B4_3630 at 4039.304 kb on + strand at 4039.304 kb on + strand, within Pf1N1B4_3630 at 4039.399 kb on - strand, within Pf1N1B4_3630 at 4039.399 kb on - strand, within Pf1N1B4_3630 at 4039.399 kb on - strand, within Pf1N1B4_3630 at 4039.599 kb on - strand, within Pf1N1B4_3630 at 4039.599 kb on - strand, within Pf1N1B4_3630 at 4039.599 kb on - strand, within Pf1N1B4_3630 at 4039.669 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 4,035,489 + Pf1N1B4_3627 0.81 +0.9 4,035,489 + Pf1N1B4_3627 0.81 -0.1 4,035,489 + Pf1N1B4_3627 0.81 -0.9 4,035,489 + Pf1N1B4_3627 0.81 +0.3 4,035,591 - -0.1 4,035,591 - -0.7 4,035,739 - Pf1N1B4_3628 0.19 +1.5 4,035,861 + Pf1N1B4_3628 0.34 -1.7 4,035,862 - Pf1N1B4_3628 0.34 -0.7 4,036,378 - -0.1 4,036,385 + -0.8 4,036,386 - +0.2 4,036,410 + -2.0 4,036,411 - -1.1 4,036,411 - -1.1 4,036,434 - -2.4 4,036,586 + +1.2 4,036,587 - -2.7 4,036,587 - +2.3 4,037,075 + Pf1N1B4_3629 0.29 -1.1 4,037,075 + Pf1N1B4_3629 0.29 -1.1 4,037,133 + Pf1N1B4_3629 0.31 -0.7 4,037,181 + Pf1N1B4_3629 0.33 -1.1 4,037,576 + Pf1N1B4_3629 0.51 -0.9 4,037,576 + Pf1N1B4_3629 0.51 -3.1 4,038,201 + Pf1N1B4_3629 0.79 -0.1 4,038,201 + Pf1N1B4_3629 0.79 -1.7 4,038,201 + Pf1N1B4_3629 0.79 +0.5 4,038,202 - +1.5 4,038,205 + Pf1N1B4_3629 0.79 -0.1 4,038,205 + Pf1N1B4_3629 0.79 +1.7 4,038,206 - Pf1N1B4_3629 0.79 -2.1 4,038,206 - Pf1N1B4_3629 0.79 -0.1 4,038,206 - Pf1N1B4_3629 0.79 +0.5 4,038,206 - Pf1N1B4_3629 0.79 -1.1 4,038,415 + Pf1N1B4_3629 0.88 -0.1 4,038,415 + Pf1N1B4_3629 0.88 -2.3 4,038,416 - Pf1N1B4_3629 0.88 -0.4 4,038,672 + +0.6 4,039,067 - Pf1N1B4_3630 0.30 -1.1 4,039,111 + Pf1N1B4_3630 0.34 +1.4 4,039,142 + Pf1N1B4_3630 0.37 +0.2 4,039,304 + -1.1 4,039,304 + Pf1N1B4_3630 0.51 -0.1 4,039,399 - Pf1N1B4_3630 0.59 -2.5 4,039,399 - Pf1N1B4_3630 0.59 +0.1 4,039,399 - Pf1N1B4_3630 0.59 -2.5 4,039,599 - Pf1N1B4_3630 0.77 -1.1 4,039,599 - Pf1N1B4_3630 0.77 -2.7 4,039,599 - Pf1N1B4_3630 0.77 +2.3 4,039,669 - -1.1
Or see this region's nucleotide sequence