Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3540 and Pf1N1B4_3541 are separated by 99 nucleotides Pf1N1B4_3541 and Pf1N1B4_3542 are separated by 191 nucleotides Pf1N1B4_3542 and Pf1N1B4_3543 overlap by 44 nucleotides
Pf1N1B4_3540 - Metal-dependent phosphohydrolase, HD subdomain, at 3,946,960 to 3,947,550
_3540
Pf1N1B4_3541 - FIG027190: Putative transmembrane protein, at 3,947,650 to 3,947,961
_3541
Pf1N1B4_3542 - Transcriptional regulator, AraC family, at 3,948,153 to 3,949,181
_3542
Pf1N1B4_3543 - Potassium uptake protein TrkH, at 3,949,138 to 3,950,592
_3543
Position (kb)
3948
3949
3950 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3947.186 kb on + strand, within Pf1N1B4_3540 at 3947.186 kb on + strand, within Pf1N1B4_3540 at 3947.186 kb on + strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.190 kb on - strand, within Pf1N1B4_3540 at 3947.282 kb on + strand, within Pf1N1B4_3540 at 3947.330 kb on + strand, within Pf1N1B4_3540 at 3947.330 kb on + strand at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand at 3947.425 kb on + strand, within Pf1N1B4_3540 at 3947.523 kb on + strand at 3947.524 kb on - strand at 3947.592 kb on + strand at 3947.652 kb on - strand at 3947.652 kb on - strand at 3947.684 kb on + strand, within Pf1N1B4_3541 at 3947.685 kb on - strand, within Pf1N1B4_3541 at 3947.685 kb on - strand, within Pf1N1B4_3541 at 3947.778 kb on + strand at 3947.778 kb on + strand, within Pf1N1B4_3541 at 3947.778 kb on + strand, within Pf1N1B4_3541 at 3947.778 kb on + strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.913 kb on - strand, within Pf1N1B4_3541 at 3948.015 kb on + strand at 3948.015 kb on + strand at 3948.016 kb on - strand at 3948.019 kb on + strand at 3948.020 kb on - strand at 3948.020 kb on - strand at 3948.057 kb on - strand at 3948.068 kb on + strand at 3948.069 kb on - strand at 3948.109 kb on + strand at 3948.113 kb on - strand at 3948.225 kb on + strand at 3948.483 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.649 kb on - strand, within Pf1N1B4_3542 at 3948.690 kb on + strand, within Pf1N1B4_3542 at 3948.691 kb on - strand at 3948.691 kb on - strand, within Pf1N1B4_3542 at 3948.786 kb on + strand, within Pf1N1B4_3542 at 3948.787 kb on - strand, within Pf1N1B4_3542 at 3948.838 kb on + strand at 3948.838 kb on + strand, within Pf1N1B4_3542 at 3948.875 kb on + strand, within Pf1N1B4_3542 at 3948.990 kb on + strand, within Pf1N1B4_3542 at 3948.990 kb on + strand at 3948.990 kb on + strand at 3948.991 kb on - strand, within Pf1N1B4_3542 at 3948.991 kb on - strand, within Pf1N1B4_3542 at 3949.029 kb on + strand at 3949.139 kb on + strand at 3949.140 kb on - strand at 3949.179 kb on + strand at 3949.448 kb on + strand, within Pf1N1B4_3543 at 3949.449 kb on - strand, within Pf1N1B4_3543 at 3949.449 kb on - strand, within Pf1N1B4_3543 at 3949.449 kb on - strand, within Pf1N1B4_3543 at 3949.449 kb on - strand, within Pf1N1B4_3543 at 3949.449 kb on - strand, within Pf1N1B4_3543 at 3949.477 kb on + strand, within Pf1N1B4_3543 at 3949.478 kb on - strand, within Pf1N1B4_3543 at 3949.489 kb on - strand, within Pf1N1B4_3543 at 3949.511 kb on + strand, within Pf1N1B4_3543 at 3949.511 kb on + strand, within Pf1N1B4_3543 at 3949.511 kb on + strand, within Pf1N1B4_3543 at 3949.511 kb on + strand, within Pf1N1B4_3543 at 3949.512 kb on - strand, within Pf1N1B4_3543 at 3949.512 kb on - strand, within Pf1N1B4_3543 at 3949.512 kb on - strand, within Pf1N1B4_3543 at 3949.583 kb on + strand, within Pf1N1B4_3543 at 3949.584 kb on - strand, within Pf1N1B4_3543 at 3949.622 kb on + strand, within Pf1N1B4_3543 at 3949.622 kb on + strand, within Pf1N1B4_3543 at 3949.758 kb on + strand, within Pf1N1B4_3543 at 3949.826 kb on + strand, within Pf1N1B4_3543 at 3949.826 kb on + strand, within Pf1N1B4_3543 at 3949.826 kb on + strand, within Pf1N1B4_3543 at 3949.826 kb on + strand, within Pf1N1B4_3543 at 3949.826 kb on + strand, within Pf1N1B4_3543 at 3949.826 kb on + strand, within Pf1N1B4_3543 at 3949.826 kb on + strand, within Pf1N1B4_3543 at 3949.827 kb on - strand, within Pf1N1B4_3543 at 3949.827 kb on - strand, within Pf1N1B4_3543 at 3949.827 kb on - strand, within Pf1N1B4_3543 at 3949.827 kb on - strand, within Pf1N1B4_3543 at 3950.027 kb on + strand, within Pf1N1B4_3543 at 3950.027 kb on + strand, within Pf1N1B4_3543 at 3950.027 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 3,947,186 + Pf1N1B4_3540 0.38 -1.1 3,947,186 + Pf1N1B4_3540 0.38 -0.3 3,947,186 + Pf1N1B4_3540 0.38 +1.2 3,947,187 - Pf1N1B4_3540 0.38 -1.1 3,947,187 - Pf1N1B4_3540 0.38 -2.1 3,947,187 - Pf1N1B4_3540 0.38 +2.7 3,947,187 - Pf1N1B4_3540 0.38 -0.1 3,947,190 - Pf1N1B4_3540 0.39 +3.2 3,947,282 + Pf1N1B4_3540 0.54 +1.9 3,947,330 + Pf1N1B4_3540 0.63 -2.1 3,947,330 + -0.1 3,947,331 - Pf1N1B4_3540 0.63 +1.9 3,947,331 - Pf1N1B4_3540 0.63 +0.6 3,947,331 - Pf1N1B4_3540 0.63 -2.7 3,947,331 - Pf1N1B4_3540 0.63 +0.5 3,947,331 - Pf1N1B4_3540 0.63 +1.1 3,947,331 - Pf1N1B4_3540 0.63 -1.4 3,947,331 - -1.1 3,947,425 + Pf1N1B4_3540 0.79 +0.5 3,947,523 + -1.4 3,947,524 - -0.1 3,947,592 + +0.1 3,947,652 - -1.1 3,947,652 - -0.1 3,947,684 + Pf1N1B4_3541 0.11 +0.9 3,947,685 - Pf1N1B4_3541 0.11 +0.9 3,947,685 - Pf1N1B4_3541 0.11 +0.9 3,947,778 + +1.9 3,947,778 + Pf1N1B4_3541 0.41 +1.5 3,947,778 + Pf1N1B4_3541 0.41 +1.2 3,947,778 + Pf1N1B4_3541 0.41 -0.1 3,947,779 - Pf1N1B4_3541 0.41 +1.1 3,947,779 - Pf1N1B4_3541 0.41 -0.1 3,947,779 - Pf1N1B4_3541 0.41 -1.7 3,947,779 - Pf1N1B4_3541 0.41 +1.5 3,947,913 - Pf1N1B4_3541 0.84 -1.5 3,948,015 + +2.5 3,948,015 + -2.7 3,948,016 - +1.1 3,948,019 + +0.2 3,948,020 - +1.9 3,948,020 - -0.1 3,948,057 - +2.5 3,948,068 + +0.5 3,948,069 - -0.5 3,948,109 + -1.1 3,948,113 - -0.4 3,948,225 + +0.6 3,948,483 + Pf1N1B4_3542 0.32 +1.6 3,948,648 + Pf1N1B4_3542 0.48 +0.5 3,948,648 + Pf1N1B4_3542 0.48 -1.1 3,948,648 + Pf1N1B4_3542 0.48 +1.5 3,948,648 + -1.1 3,948,648 + Pf1N1B4_3542 0.48 -0.7 3,948,648 + Pf1N1B4_3542 0.48 -1.4 3,948,648 + Pf1N1B4_3542 0.48 +0.5 3,948,648 + Pf1N1B4_3542 0.48 -0.1 3,948,649 - Pf1N1B4_3542 0.48 -2.9 3,948,690 + Pf1N1B4_3542 0.52 +0.9 3,948,691 - -0.1 3,948,691 - Pf1N1B4_3542 0.52 -1.1 3,948,786 + Pf1N1B4_3542 0.62 -3.5 3,948,787 - Pf1N1B4_3542 0.62 -0.1 3,948,838 + -1.1 3,948,838 + Pf1N1B4_3542 0.67 -0.3 3,948,875 + Pf1N1B4_3542 0.70 +0.5 3,948,990 + Pf1N1B4_3542 0.81 +0.9 3,948,990 + +0.9 3,948,990 + -1.1 3,948,991 - Pf1N1B4_3542 0.81 +0.5 3,948,991 - Pf1N1B4_3542 0.81 -0.5 3,949,029 + -0.7 3,949,139 + -1.1 3,949,140 - -1.4 3,949,179 + +0.2 3,949,448 + Pf1N1B4_3543 0.21 +1.1 3,949,449 - Pf1N1B4_3543 0.21 +2.2 3,949,449 - Pf1N1B4_3543 0.21 -1.5 3,949,449 - Pf1N1B4_3543 0.21 +0.9 3,949,449 - Pf1N1B4_3543 0.21 +0.6 3,949,449 - Pf1N1B4_3543 0.21 -0.8 3,949,477 + Pf1N1B4_3543 0.23 -2.1 3,949,478 - Pf1N1B4_3543 0.23 -0.8 3,949,489 - Pf1N1B4_3543 0.24 +0.3 3,949,511 + Pf1N1B4_3543 0.26 +1.6 3,949,511 + Pf1N1B4_3543 0.26 +1.2 3,949,511 + Pf1N1B4_3543 0.26 +1.2 3,949,511 + Pf1N1B4_3543 0.26 +1.1 3,949,512 - Pf1N1B4_3543 0.26 -2.7 3,949,512 - Pf1N1B4_3543 0.26 +1.9 3,949,512 - Pf1N1B4_3543 0.26 +0.6 3,949,583 + Pf1N1B4_3543 0.31 +0.3 3,949,584 - Pf1N1B4_3543 0.31 +1.9 3,949,622 + Pf1N1B4_3543 0.33 -0.4 3,949,622 + Pf1N1B4_3543 0.33 +2.0 3,949,758 + Pf1N1B4_3543 0.43 -0.1 3,949,826 + Pf1N1B4_3543 0.47 -0.4 3,949,826 + Pf1N1B4_3543 0.47 -1.4 3,949,826 + Pf1N1B4_3543 0.47 -0.7 3,949,826 + Pf1N1B4_3543 0.47 -0.1 3,949,826 + Pf1N1B4_3543 0.47 -0.1 3,949,826 + Pf1N1B4_3543 0.47 +2.2 3,949,826 + Pf1N1B4_3543 0.47 -0.1 3,949,827 - Pf1N1B4_3543 0.47 -2.1 3,949,827 - Pf1N1B4_3543 0.47 -1.0 3,949,827 - Pf1N1B4_3543 0.47 -0.4 3,949,827 - Pf1N1B4_3543 0.47 -2.1 3,950,027 + Pf1N1B4_3543 0.61 -1.7 3,950,027 + Pf1N1B4_3543 0.61 -2.9 3,950,027 + -1.1
Or see this region's nucleotide sequence