Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_339 and Pf1N1B4_340 overlap by 4 nucleotides Pf1N1B4_340 and Pf1N1B4_341 are separated by 56 nucleotides Pf1N1B4_341 and Pf1N1B4_342 overlap by 4 nucleotides Pf1N1B4_342 and Pf1N1B4_343 overlap by 4 nucleotides
Pf1N1B4_339 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 403,154 to 404,116
_339
Pf1N1B4_340 - Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-), at 404,113 to 405,036
_340
Pf1N1B4_341 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 405,093 to 405,983
_341
Pf1N1B4_342 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 405,980 to 406,873
_342
Pf1N1B4_343 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 406,870 to 407,946
_343
Position (kb) 405
406 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 404.132 kb on - strand at 404.155 kb on - strand at 404.155 kb on - strand at 404.219 kb on - strand, within Pf1N1B4_340 at 404.383 kb on - strand, within Pf1N1B4_340 at 404.427 kb on - strand, within Pf1N1B4_340 at 404.427 kb on - strand, within Pf1N1B4_340 at 404.460 kb on - strand, within Pf1N1B4_340 at 404.466 kb on - strand, within Pf1N1B4_340 at 404.555 kb on + strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand at 404.559 kb on + strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.588 kb on + strand, within Pf1N1B4_340 at 404.589 kb on - strand, within Pf1N1B4_340 at 404.589 kb on - strand, within Pf1N1B4_340 at 404.589 kb on - strand, within Pf1N1B4_340 at 404.669 kb on + strand, within Pf1N1B4_340 at 404.669 kb on + strand, within Pf1N1B4_340 at 404.921 kb on + strand, within Pf1N1B4_340 at 404.921 kb on + strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.993 kb on + strand at 404.993 kb on + strand at 404.993 kb on + strand at 404.994 kb on - strand at 404.994 kb on - strand at 404.994 kb on - strand at 404.994 kb on - strand at 405.029 kb on - strand at 405.029 kb on - strand at 405.128 kb on - strand at 405.128 kb on - strand at 405.128 kb on - strand at 405.359 kb on - strand at 405.443 kb on - strand, within Pf1N1B4_341 at 405.443 kb on - strand, within Pf1N1B4_341 at 405.443 kb on - strand, within Pf1N1B4_341 at 405.448 kb on + strand, within Pf1N1B4_341 at 405.448 kb on + strand, within Pf1N1B4_341 at 405.448 kb on + strand, within Pf1N1B4_341 at 405.449 kb on - strand, within Pf1N1B4_341 at 405.476 kb on + strand, within Pf1N1B4_341 at 405.477 kb on - strand, within Pf1N1B4_341 at 405.574 kb on - strand, within Pf1N1B4_341 at 405.635 kb on - strand, within Pf1N1B4_341 at 405.635 kb on - strand, within Pf1N1B4_341 at 405.726 kb on - strand, within Pf1N1B4_341 at 405.740 kb on - strand, within Pf1N1B4_341 at 405.815 kb on + strand at 405.815 kb on + strand, within Pf1N1B4_341 at 405.816 kb on - strand, within Pf1N1B4_341 at 405.966 kb on - strand at 405.985 kb on + strand at 405.985 kb on + strand at 406.896 kb on - strand at 406.943 kb on - strand at 406.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 404,132 - +2.1 404,155 - -1.1 404,155 - -1.7 404,219 - Pf1N1B4_340 0.11 -2.9 404,383 - Pf1N1B4_340 0.29 +2.3 404,427 - Pf1N1B4_340 0.34 +0.3 404,427 - Pf1N1B4_340 0.34 -1.1 404,460 - Pf1N1B4_340 0.38 -0.8 404,466 - Pf1N1B4_340 0.38 -1.1 404,555 + Pf1N1B4_340 0.48 -1.7 404,556 - Pf1N1B4_340 0.48 -1.1 404,556 - Pf1N1B4_340 0.48 -0.8 404,556 - Pf1N1B4_340 0.48 -2.1 404,556 - Pf1N1B4_340 0.48 -1.3 404,556 - Pf1N1B4_340 0.48 +0.8 404,556 - Pf1N1B4_340 0.48 +0.7 404,556 - Pf1N1B4_340 0.48 -2.1 404,556 - -1.1 404,559 + Pf1N1B4_340 0.48 -2.7 404,560 - Pf1N1B4_340 0.48 -1.4 404,560 - Pf1N1B4_340 0.48 +1.6 404,560 - Pf1N1B4_340 0.48 -2.4 404,560 - Pf1N1B4_340 0.48 +1.7 404,560 - Pf1N1B4_340 0.48 +1.7 404,588 + Pf1N1B4_340 0.51 +0.9 404,589 - Pf1N1B4_340 0.52 +2.4 404,589 - Pf1N1B4_340 0.52 -0.1 404,589 - Pf1N1B4_340 0.52 +1.1 404,669 + Pf1N1B4_340 0.60 -2.9 404,669 + Pf1N1B4_340 0.60 -0.3 404,921 + Pf1N1B4_340 0.87 +0.2 404,921 + Pf1N1B4_340 0.87 -0.1 404,922 - Pf1N1B4_340 0.88 -0.5 404,922 - Pf1N1B4_340 0.88 +0.7 404,922 - Pf1N1B4_340 0.88 -0.1 404,922 - Pf1N1B4_340 0.88 -0.8 404,922 - Pf1N1B4_340 0.88 +0.7 404,922 - Pf1N1B4_340 0.88 -1.1 404,993 + -0.1 404,993 + -1.1 404,993 + +0.5 404,994 - -0.1 404,994 - -1.4 404,994 - -1.7 404,994 - +1.1 405,029 - -0.1 405,029 - +0.8 405,128 - +0.5 405,128 - +0.9 405,128 - +0.9 405,359 - -1.7 405,443 - Pf1N1B4_341 0.39 -2.7 405,443 - Pf1N1B4_341 0.39 +0.9 405,443 - Pf1N1B4_341 0.39 +0.9 405,448 + Pf1N1B4_341 0.40 -2.1 405,448 + Pf1N1B4_341 0.40 +0.3 405,448 + Pf1N1B4_341 0.40 -1.5 405,449 - Pf1N1B4_341 0.40 -0.1 405,476 + Pf1N1B4_341 0.43 -2.1 405,477 - Pf1N1B4_341 0.43 -1.1 405,574 - Pf1N1B4_341 0.54 -2.3 405,635 - Pf1N1B4_341 0.61 -1.7 405,635 - Pf1N1B4_341 0.61 -2.4 405,726 - Pf1N1B4_341 0.71 -1.8 405,740 - Pf1N1B4_341 0.73 +1.8 405,815 + -0.1 405,815 + Pf1N1B4_341 0.81 +3.8 405,816 - Pf1N1B4_341 0.81 +0.4 405,966 - +0.6 405,985 + -0.5 405,985 + -1.1 406,896 - -2.4 406,943 - +0.3 406,943 - +0.7
Or see this region's nucleotide sequence