Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_341

Experiment: Fusidic 0.125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_339 and Pf1N1B4_340 overlap by 4 nucleotidesPf1N1B4_340 and Pf1N1B4_341 are separated by 56 nucleotidesPf1N1B4_341 and Pf1N1B4_342 overlap by 4 nucleotidesPf1N1B4_342 and Pf1N1B4_343 overlap by 4 nucleotides Pf1N1B4_339 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 403,154 to 404,116 _339 Pf1N1B4_340 - Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-), at 404,113 to 405,036 _340 Pf1N1B4_341 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 405,093 to 405,983 _341 Pf1N1B4_342 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 405,980 to 406,873 _342 Pf1N1B4_343 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 406,870 to 407,946 _343 Position (kb) 405 406Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 404.132 kb on - strandat 404.155 kb on - strandat 404.155 kb on - strandat 404.219 kb on - strand, within Pf1N1B4_340at 404.383 kb on - strand, within Pf1N1B4_340at 404.427 kb on - strand, within Pf1N1B4_340at 404.427 kb on - strand, within Pf1N1B4_340at 404.460 kb on - strand, within Pf1N1B4_340at 404.466 kb on - strand, within Pf1N1B4_340at 404.555 kb on + strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strand, within Pf1N1B4_340at 404.556 kb on - strandat 404.559 kb on + strand, within Pf1N1B4_340at 404.560 kb on - strand, within Pf1N1B4_340at 404.560 kb on - strand, within Pf1N1B4_340at 404.560 kb on - strand, within Pf1N1B4_340at 404.560 kb on - strand, within Pf1N1B4_340at 404.560 kb on - strand, within Pf1N1B4_340at 404.588 kb on + strand, within Pf1N1B4_340at 404.589 kb on - strand, within Pf1N1B4_340at 404.589 kb on - strand, within Pf1N1B4_340at 404.589 kb on - strand, within Pf1N1B4_340at 404.669 kb on + strand, within Pf1N1B4_340at 404.669 kb on + strand, within Pf1N1B4_340at 404.921 kb on + strand, within Pf1N1B4_340at 404.921 kb on + strand, within Pf1N1B4_340at 404.922 kb on - strand, within Pf1N1B4_340at 404.922 kb on - strand, within Pf1N1B4_340at 404.922 kb on - strand, within Pf1N1B4_340at 404.922 kb on - strand, within Pf1N1B4_340at 404.922 kb on - strand, within Pf1N1B4_340at 404.922 kb on - strand, within Pf1N1B4_340at 404.993 kb on + strandat 404.993 kb on + strandat 404.993 kb on + strandat 404.994 kb on - strandat 404.994 kb on - strandat 404.994 kb on - strandat 404.994 kb on - strandat 405.029 kb on - strandat 405.029 kb on - strandat 405.128 kb on - strandat 405.128 kb on - strandat 405.128 kb on - strandat 405.359 kb on - strandat 405.443 kb on - strand, within Pf1N1B4_341at 405.443 kb on - strand, within Pf1N1B4_341at 405.443 kb on - strand, within Pf1N1B4_341at 405.448 kb on + strand, within Pf1N1B4_341at 405.448 kb on + strand, within Pf1N1B4_341at 405.448 kb on + strand, within Pf1N1B4_341at 405.449 kb on - strand, within Pf1N1B4_341at 405.476 kb on + strand, within Pf1N1B4_341at 405.477 kb on - strand, within Pf1N1B4_341at 405.574 kb on - strand, within Pf1N1B4_341at 405.635 kb on - strand, within Pf1N1B4_341at 405.635 kb on - strand, within Pf1N1B4_341at 405.726 kb on - strand, within Pf1N1B4_341at 405.740 kb on - strand, within Pf1N1B4_341at 405.815 kb on + strandat 405.815 kb on + strand, within Pf1N1B4_341at 405.816 kb on - strand, within Pf1N1B4_341at 405.966 kb on - strandat 405.985 kb on + strandat 405.985 kb on + strandat 406.896 kb on - strandat 406.943 kb on - strandat 406.943 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml
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404,132 - +2.1
404,155 - -1.1
404,155 - -1.7
404,219 - Pf1N1B4_340 0.11 -2.9
404,383 - Pf1N1B4_340 0.29 +2.3
404,427 - Pf1N1B4_340 0.34 +0.3
404,427 - Pf1N1B4_340 0.34 -1.1
404,460 - Pf1N1B4_340 0.38 -0.8
404,466 - Pf1N1B4_340 0.38 -1.1
404,555 + Pf1N1B4_340 0.48 -1.7
404,556 - Pf1N1B4_340 0.48 -1.1
404,556 - Pf1N1B4_340 0.48 -0.8
404,556 - Pf1N1B4_340 0.48 -2.1
404,556 - Pf1N1B4_340 0.48 -1.3
404,556 - Pf1N1B4_340 0.48 +0.8
404,556 - Pf1N1B4_340 0.48 +0.7
404,556 - Pf1N1B4_340 0.48 -2.1
404,556 - -1.1
404,559 + Pf1N1B4_340 0.48 -2.7
404,560 - Pf1N1B4_340 0.48 -1.4
404,560 - Pf1N1B4_340 0.48 +1.6
404,560 - Pf1N1B4_340 0.48 -2.4
404,560 - Pf1N1B4_340 0.48 +1.7
404,560 - Pf1N1B4_340 0.48 +1.7
404,588 + Pf1N1B4_340 0.51 +0.9
404,589 - Pf1N1B4_340 0.52 +2.4
404,589 - Pf1N1B4_340 0.52 -0.1
404,589 - Pf1N1B4_340 0.52 +1.1
404,669 + Pf1N1B4_340 0.60 -2.9
404,669 + Pf1N1B4_340 0.60 -0.3
404,921 + Pf1N1B4_340 0.87 +0.2
404,921 + Pf1N1B4_340 0.87 -0.1
404,922 - Pf1N1B4_340 0.88 -0.5
404,922 - Pf1N1B4_340 0.88 +0.7
404,922 - Pf1N1B4_340 0.88 -0.1
404,922 - Pf1N1B4_340 0.88 -0.8
404,922 - Pf1N1B4_340 0.88 +0.7
404,922 - Pf1N1B4_340 0.88 -1.1
404,993 + -0.1
404,993 + -1.1
404,993 + +0.5
404,994 - -0.1
404,994 - -1.4
404,994 - -1.7
404,994 - +1.1
405,029 - -0.1
405,029 - +0.8
405,128 - +0.5
405,128 - +0.9
405,128 - +0.9
405,359 - -1.7
405,443 - Pf1N1B4_341 0.39 -2.7
405,443 - Pf1N1B4_341 0.39 +0.9
405,443 - Pf1N1B4_341 0.39 +0.9
405,448 + Pf1N1B4_341 0.40 -2.1
405,448 + Pf1N1B4_341 0.40 +0.3
405,448 + Pf1N1B4_341 0.40 -1.5
405,449 - Pf1N1B4_341 0.40 -0.1
405,476 + Pf1N1B4_341 0.43 -2.1
405,477 - Pf1N1B4_341 0.43 -1.1
405,574 - Pf1N1B4_341 0.54 -2.3
405,635 - Pf1N1B4_341 0.61 -1.7
405,635 - Pf1N1B4_341 0.61 -2.4
405,726 - Pf1N1B4_341 0.71 -1.8
405,740 - Pf1N1B4_341 0.73 +1.8
405,815 + -0.1
405,815 + Pf1N1B4_341 0.81 +3.8
405,816 - Pf1N1B4_341 0.81 +0.4
405,966 - +0.6
405,985 + -0.5
405,985 + -1.1
406,896 - -2.4
406,943 - +0.3
406,943 - +0.7

Or see this region's nucleotide sequence