Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_33 and Pf1N1B4_34 are separated by 152 nucleotides Pf1N1B4_34 and Pf1N1B4_35 are separated by 432 nucleotides Pf1N1B4_35 and Pf1N1B4_36 are separated by 3 nucleotides
Pf1N1B4_33 - Outer membrane component of tripartite multidrug resistance system, at 37,992 to 39,422
_33
Pf1N1B4_34 - Xanthine permease, at 39,575 to 41,029
_34
Pf1N1B4_35 - hypothetical protein, at 41,462 to 41,629
_35
Pf1N1B4_36 - hypothetical protein, at 41,633 to 41,977
_36
Position (kb) 39
40
41
42 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 38.703 kb on + strand at 38.703 kb on + strand at 38.703 kb on + strand, within Pf1N1B4_33 at 38.703 kb on + strand, within Pf1N1B4_33 at 38.704 kb on - strand at 38.704 kb on - strand at 38.704 kb on - strand, within Pf1N1B4_33 at 38.706 kb on - strand, within Pf1N1B4_33 at 38.721 kb on - strand, within Pf1N1B4_33 at 38.863 kb on - strand, within Pf1N1B4_33 at 38.904 kb on - strand, within Pf1N1B4_33 at 38.968 kb on + strand, within Pf1N1B4_33 at 38.968 kb on + strand, within Pf1N1B4_33 at 38.969 kb on - strand at 39.156 kb on + strand, within Pf1N1B4_33 at 39.189 kb on + strand, within Pf1N1B4_33 at 39.548 kb on + strand at 39.691 kb on - strand at 39.883 kb on - strand, within Pf1N1B4_34 at 40.501 kb on + strand, within Pf1N1B4_34 at 40.501 kb on + strand, within Pf1N1B4_34 at 40.501 kb on + strand, within Pf1N1B4_34 at 40.995 kb on + strand at 40.995 kb on + strand at 40.996 kb on - strand at 40.996 kb on - strand at 40.996 kb on - strand at 41.030 kb on - strand at 41.133 kb on - strand at 41.167 kb on - strand at 41.169 kb on - strand at 41.249 kb on + strand at 41.250 kb on - strand at 41.250 kb on - strand at 41.250 kb on - strand at 41.330 kb on - strand at 41.336 kb on - strand at 41.336 kb on - strand at 41.543 kb on + strand, within Pf1N1B4_35 at 41.544 kb on - strand at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.544 kb on - strand, within Pf1N1B4_35 at 41.549 kb on + strand at 41.615 kb on + strand at 41.615 kb on + strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.616 kb on - strand at 41.618 kb on - strand at 41.618 kb on - strand at 41.646 kb on - strand at 41.731 kb on - strand, within Pf1N1B4_36 at 41.791 kb on + strand, within Pf1N1B4_36 at 41.792 kb on - strand, within Pf1N1B4_36 at 41.975 kb on + strand at 41.976 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 38,703 + +1.4 38,703 + -1.2 38,703 + Pf1N1B4_33 0.50 +0.8 38,703 + Pf1N1B4_33 0.50 -0.5 38,704 - +1.4 38,704 - -1.8 38,704 - Pf1N1B4_33 0.50 -0.6 38,706 - Pf1N1B4_33 0.50 -0.2 38,721 - Pf1N1B4_33 0.51 -1.8 38,863 - Pf1N1B4_33 0.61 +0.4 38,904 - Pf1N1B4_33 0.64 +0.0 38,968 + Pf1N1B4_33 0.68 +1.1 38,968 + Pf1N1B4_33 0.68 -1.8 38,969 - -1.2 39,156 + Pf1N1B4_33 0.81 +1.4 39,189 + Pf1N1B4_33 0.84 +1.8 39,548 + -2.5 39,691 - -1.2 39,883 - Pf1N1B4_34 0.21 -1.5 40,501 + Pf1N1B4_34 0.64 -0.2 40,501 + Pf1N1B4_34 0.64 -1.2 40,501 + Pf1N1B4_34 0.64 -1.0 40,995 + -2.2 40,995 + +0.3 40,996 - +0.0 40,996 - -2.5 40,996 - -1.5 41,030 - -0.2 41,133 - +1.4 41,167 - +0.8 41,169 - +0.1 41,249 + +2.8 41,250 - +0.2 41,250 - +0.6 41,250 - -0.2 41,330 - +0.8 41,336 - -2.2 41,336 - -1.2 41,543 + Pf1N1B4_35 0.48 +0.8 41,544 - -0.2 41,544 - Pf1N1B4_35 0.49 -0.9 41,544 - Pf1N1B4_35 0.49 +2.4 41,544 - Pf1N1B4_35 0.49 -0.4 41,544 - Pf1N1B4_35 0.49 -2.2 41,544 - Pf1N1B4_35 0.49 -0.2 41,544 - Pf1N1B4_35 0.49 -0.2 41,544 - Pf1N1B4_35 0.49 -2.2 41,549 + -1.8 41,615 + -1.2 41,615 + +2.8 41,616 - +0.3 41,616 - -1.2 41,616 - -0.8 41,616 - +1.4 41,616 - -0.8 41,616 - -0.2 41,616 - -0.7 41,618 - -0.2 41,618 - -0.2 41,646 - -1.2 41,731 - Pf1N1B4_36 0.28 +1.4 41,791 + Pf1N1B4_36 0.46 -0.2 41,792 - Pf1N1B4_36 0.46 +1.4 41,975 + -1.8 41,976 - -2.8
Or see this region's nucleotide sequence