Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3387 and Pf1N1B4_3388 are separated by 193 nucleotides Pf1N1B4_3388 and Pf1N1B4_3389 are separated by 342 nucleotides
Pf1N1B4_3387 - Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-), at 3,776,698 to 3,778,419
_3387
Pf1N1B4_3388 - ATP-dependent RNA helicase HrpA (EC 3.6.4.13), at 3,778,613 to 3,782,524
_3388
Pf1N1B4_3389 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180), at 3,782,867 to 3,783,988
_3389
Position (kb)
3778
3779
3780
3781
3782
3783 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3777.717 kb on + strand, within Pf1N1B4_3387 at 3777.772 kb on + strand, within Pf1N1B4_3387 at 3777.773 kb on - strand, within Pf1N1B4_3387 at 3777.921 kb on + strand, within Pf1N1B4_3387 at 3777.976 kb on + strand, within Pf1N1B4_3387 at 3778.025 kb on - strand, within Pf1N1B4_3387 at 3778.025 kb on - strand, within Pf1N1B4_3387 at 3778.025 kb on - strand, within Pf1N1B4_3387 at 3778.059 kb on + strand, within Pf1N1B4_3387 at 3778.124 kb on - strand, within Pf1N1B4_3387 at 3778.124 kb on - strand, within Pf1N1B4_3387 at 3778.124 kb on - strand, within Pf1N1B4_3387 at 3778.124 kb on - strand, within Pf1N1B4_3387 at 3778.129 kb on + strand, within Pf1N1B4_3387 at 3778.130 kb on - strand at 3778.241 kb on + strand, within Pf1N1B4_3387 at 3778.241 kb on + strand, within Pf1N1B4_3387 at 3778.242 kb on - strand, within Pf1N1B4_3387 at 3778.321 kb on + strand at 3778.321 kb on + strand at 3778.593 kb on - strand at 3778.607 kb on + strand at 3778.608 kb on - strand at 3778.608 kb on - strand at 3779.168 kb on + strand, within Pf1N1B4_3388 at 3779.169 kb on - strand, within Pf1N1B4_3388 at 3779.353 kb on + strand, within Pf1N1B4_3388 at 3779.365 kb on - strand, within Pf1N1B4_3388 at 3779.485 kb on + strand, within Pf1N1B4_3388 at 3779.485 kb on + strand, within Pf1N1B4_3388 at 3779.486 kb on - strand, within Pf1N1B4_3388 at 3779.604 kb on - strand, within Pf1N1B4_3388 at 3779.604 kb on - strand, within Pf1N1B4_3388 at 3779.604 kb on - strand, within Pf1N1B4_3388 at 3779.604 kb on - strand, within Pf1N1B4_3388 at 3779.604 kb on - strand, within Pf1N1B4_3388 at 3779.675 kb on - strand, within Pf1N1B4_3388 at 3779.717 kb on + strand, within Pf1N1B4_3388 at 3779.822 kb on - strand, within Pf1N1B4_3388 at 3779.867 kb on + strand, within Pf1N1B4_3388 at 3779.867 kb on + strand, within Pf1N1B4_3388 at 3779.867 kb on + strand, within Pf1N1B4_3388 at 3779.868 kb on - strand, within Pf1N1B4_3388 at 3779.992 kb on + strand, within Pf1N1B4_3388 at 3780.295 kb on + strand, within Pf1N1B4_3388 at 3780.352 kb on + strand, within Pf1N1B4_3388 at 3780.381 kb on - strand, within Pf1N1B4_3388 at 3780.545 kb on + strand, within Pf1N1B4_3388 at 3780.545 kb on + strand, within Pf1N1B4_3388 at 3780.545 kb on + strand, within Pf1N1B4_3388 at 3780.654 kb on - strand at 3780.801 kb on + strand, within Pf1N1B4_3388 at 3780.801 kb on + strand at 3781.010 kb on + strand, within Pf1N1B4_3388 at 3781.010 kb on + strand, within Pf1N1B4_3388 at 3781.010 kb on + strand, within Pf1N1B4_3388 at 3781.010 kb on + strand, within Pf1N1B4_3388 at 3781.011 kb on - strand at 3781.011 kb on - strand, within Pf1N1B4_3388 at 3781.011 kb on - strand, within Pf1N1B4_3388 at 3781.011 kb on - strand, within Pf1N1B4_3388 at 3781.202 kb on + strand, within Pf1N1B4_3388 at 3781.202 kb on + strand, within Pf1N1B4_3388 at 3781.203 kb on - strand, within Pf1N1B4_3388 at 3781.455 kb on + strand, within Pf1N1B4_3388 at 3781.455 kb on + strand, within Pf1N1B4_3388 at 3781.455 kb on + strand, within Pf1N1B4_3388 at 3781.456 kb on - strand, within Pf1N1B4_3388 at 3781.740 kb on - strand, within Pf1N1B4_3388 at 3781.913 kb on + strand, within Pf1N1B4_3388 at 3781.913 kb on + strand, within Pf1N1B4_3388 at 3781.913 kb on + strand, within Pf1N1B4_3388 at 3781.913 kb on + strand, within Pf1N1B4_3388 at 3781.913 kb on + strand, within Pf1N1B4_3388 at 3781.914 kb on - strand, within Pf1N1B4_3388 at 3782.201 kb on + strand at 3782.255 kb on + strand at 3782.352 kb on - strand at 3782.552 kb on + strand at 3782.552 kb on + strand at 3782.553 kb on - strand at 3782.553 kb on - strand at 3782.557 kb on + strand at 3782.558 kb on - strand at 3782.656 kb on + strand at 3782.657 kb on - strand at 3782.657 kb on - strand at 3782.713 kb on + strand at 3782.713 kb on + strand at 3782.714 kb on - strand at 3782.715 kb on + strand at 3782.766 kb on + strand at 3782.767 kb on - strand at 3782.801 kb on + strand at 3782.801 kb on + strand at 3782.802 kb on - strand at 3782.802 kb on - strand at 3782.802 kb on - strand at 3782.802 kb on - strand at 3782.820 kb on + strand at 3782.820 kb on + strand at 3782.820 kb on + strand at 3782.821 kb on - strand at 3782.821 kb on - strand at 3782.821 kb on - strand at 3782.821 kb on - strand at 3782.873 kb on - strand at 3782.873 kb on - strand at 3782.901 kb on - strand at 3782.901 kb on - strand at 3782.901 kb on - strand at 3782.901 kb on - strand at 3782.954 kb on + strand at 3782.954 kb on + strand at 3782.954 kb on + strand at 3783.524 kb on + strand, within Pf1N1B4_3389 at 3783.524 kb on + strand, within Pf1N1B4_3389
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 3,777,717 + Pf1N1B4_3387 0.59 +0.8 3,777,772 + Pf1N1B4_3387 0.62 -1.6 3,777,773 - Pf1N1B4_3387 0.62 -1.0 3,777,921 + Pf1N1B4_3387 0.71 +3.4 3,777,976 + Pf1N1B4_3387 0.74 +2.5 3,778,025 - Pf1N1B4_3387 0.77 +0.6 3,778,025 - Pf1N1B4_3387 0.77 -2.1 3,778,025 - Pf1N1B4_3387 0.77 -2.6 3,778,059 + Pf1N1B4_3387 0.79 -1.6 3,778,124 - Pf1N1B4_3387 0.83 -1.6 3,778,124 - Pf1N1B4_3387 0.83 -0.6 3,778,124 - Pf1N1B4_3387 0.83 -2.6 3,778,124 - Pf1N1B4_3387 0.83 -0.7 3,778,129 + Pf1N1B4_3387 0.83 -1.0 3,778,130 - +0.7 3,778,241 + Pf1N1B4_3387 0.90 +1.2 3,778,241 + Pf1N1B4_3387 0.90 -0.0 3,778,242 - Pf1N1B4_3387 0.90 -0.7 3,778,321 + +1.6 3,778,321 + +1.6 3,778,593 - -1.6 3,778,607 + +1.6 3,778,608 - -2.8 3,778,608 - -0.7 3,779,168 + Pf1N1B4_3388 0.14 -3.6 3,779,169 - Pf1N1B4_3388 0.14 -1.6 3,779,353 + Pf1N1B4_3388 0.19 -1.3 3,779,365 - Pf1N1B4_3388 0.19 -1.8 3,779,485 + Pf1N1B4_3388 0.22 -2.8 3,779,485 + Pf1N1B4_3388 0.22 -0.0 3,779,486 - Pf1N1B4_3388 0.22 -1.8 3,779,604 - Pf1N1B4_3388 0.25 -2.0 3,779,604 - Pf1N1B4_3388 0.25 -3.3 3,779,604 - Pf1N1B4_3388 0.25 -1.6 3,779,604 - Pf1N1B4_3388 0.25 -2.0 3,779,604 - Pf1N1B4_3388 0.25 -1.3 3,779,675 - Pf1N1B4_3388 0.27 -2.3 3,779,717 + Pf1N1B4_3388 0.28 -2.8 3,779,822 - Pf1N1B4_3388 0.31 -2.6 3,779,867 + Pf1N1B4_3388 0.32 -2.6 3,779,867 + Pf1N1B4_3388 0.32 -2.8 3,779,867 + Pf1N1B4_3388 0.32 -3.0 3,779,868 - Pf1N1B4_3388 0.32 -1.0 3,779,992 + Pf1N1B4_3388 0.35 -1.6 3,780,295 + Pf1N1B4_3388 0.43 -2.3 3,780,352 + Pf1N1B4_3388 0.44 -3.0 3,780,381 - Pf1N1B4_3388 0.45 +0.1 3,780,545 + Pf1N1B4_3388 0.49 -2.3 3,780,545 + Pf1N1B4_3388 0.49 -1.6 3,780,545 + Pf1N1B4_3388 0.49 -3.5 3,780,654 - -0.0 3,780,801 + Pf1N1B4_3388 0.56 -2.6 3,780,801 + -0.0 3,781,010 + Pf1N1B4_3388 0.61 -2.0 3,781,010 + Pf1N1B4_3388 0.61 -2.6 3,781,010 + Pf1N1B4_3388 0.61 -2.3 3,781,010 + Pf1N1B4_3388 0.61 -2.5 3,781,011 - -0.0 3,781,011 - Pf1N1B4_3388 0.61 +0.2 3,781,011 - Pf1N1B4_3388 0.61 -1.3 3,781,011 - Pf1N1B4_3388 0.61 -1.6 3,781,202 + Pf1N1B4_3388 0.66 -1.0 3,781,202 + Pf1N1B4_3388 0.66 -1.6 3,781,203 - Pf1N1B4_3388 0.66 -1.0 3,781,455 + Pf1N1B4_3388 0.73 -1.6 3,781,455 + Pf1N1B4_3388 0.73 -3.0 3,781,455 + Pf1N1B4_3388 0.73 -2.0 3,781,456 - Pf1N1B4_3388 0.73 -1.0 3,781,740 - Pf1N1B4_3388 0.80 -0.0 3,781,913 + Pf1N1B4_3388 0.84 -2.3 3,781,913 + Pf1N1B4_3388 0.84 -3.7 3,781,913 + Pf1N1B4_3388 0.84 -2.0 3,781,913 + Pf1N1B4_3388 0.84 -0.0 3,781,913 + Pf1N1B4_3388 0.84 -4.8 3,781,914 - Pf1N1B4_3388 0.84 -3.0 3,782,201 + -2.3 3,782,255 + -0.0 3,782,352 - -0.0 3,782,552 + -1.3 3,782,552 + +3.0 3,782,553 - -2.8 3,782,553 - -1.6 3,782,557 + +1.0 3,782,558 - -0.0 3,782,656 + -0.4 3,782,657 - -1.8 3,782,657 - -0.0 3,782,713 + -0.2 3,782,713 + +2.2 3,782,714 - -1.6 3,782,715 + +2.5 3,782,766 + +0.2 3,782,767 - -1.0 3,782,801 + -1.6 3,782,801 + -1.6 3,782,802 - -1.6 3,782,802 - -3.0 3,782,802 - -1.9 3,782,802 - -0.9 3,782,820 + -0.3 3,782,820 + -2.0 3,782,820 + -1.0 3,782,821 - -1.0 3,782,821 - -0.6 3,782,821 - -1.3 3,782,821 - -1.0 3,782,873 - -1.0 3,782,873 - -0.0 3,782,901 - -1.6 3,782,901 - -2.8 3,782,901 - -0.6 3,782,901 - -1.3 3,782,954 + -2.0 3,782,954 + -3.0 3,782,954 + +1.1 3,783,524 + Pf1N1B4_3389 0.59 -2.0 3,783,524 + Pf1N1B4_3389 0.59 -1.6
Or see this region's nucleotide sequence