Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_325 and Pf1N1B4_326 are separated by 443 nucleotides Pf1N1B4_326 and Pf1N1B4_327 are separated by 2 nucleotides Pf1N1B4_327 and Pf1N1B4_328 are separated by 905 nucleotides
Pf1N1B4_325 - D amino acid oxidase (DAO) family (EC 1.4.3.3), at 383,627 to 384,769
_325
Pf1N1B4_326 - Glycosyl transferase, family 2:GtrA-like protein, at 385,213 to 385,503
_326
Pf1N1B4_327 - hypothetical protein, at 385,506 to 386,450
_327
Pf1N1B4_328 - Glucosyltransferase (side chain biosynthesis) (EC 2.4.1.-), at 387,356 to 388,624
_328
Position (kb) 385
386
387 Strain fitness (log2 ratio) -2
-1
0
1
2 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.525 kb on + strand, within Pf1N1B4_325 at 384.526 kb on - strand, within Pf1N1B4_325 at 384.526 kb on - strand, within Pf1N1B4_325 at 384.526 kb on - strand, within Pf1N1B4_325 at 384.533 kb on + strand, within Pf1N1B4_325 at 384.533 kb on + strand, within Pf1N1B4_325 at 384.560 kb on + strand, within Pf1N1B4_325 at 384.560 kb on + strand, within Pf1N1B4_325 at 384.560 kb on + strand, within Pf1N1B4_325 at 384.561 kb on - strand, within Pf1N1B4_325 at 384.561 kb on - strand, within Pf1N1B4_325 at 384.564 kb on + strand, within Pf1N1B4_325 at 384.565 kb on - strand at 384.565 kb on - strand, within Pf1N1B4_325 at 384.596 kb on - strand, within Pf1N1B4_325 at 384.663 kb on - strand at 384.695 kb on + strand at 384.696 kb on - strand at 384.696 kb on - strand at 384.696 kb on - strand at 384.847 kb on + strand at 384.850 kb on - strand at 385.061 kb on + strand at 385.062 kb on - strand at 385.196 kb on + strand at 385.587 kb on + strand at 385.590 kb on - strand at 385.787 kb on - strand, within Pf1N1B4_327 at 385.787 kb on - strand, within Pf1N1B4_327 at 385.904 kb on - strand, within Pf1N1B4_327 at 385.904 kb on - strand, within Pf1N1B4_327 at 386.938 kb on + strand at 386.939 kb on - strand at 386.940 kb on + strand at 387.303 kb on + strand at 387.304 kb on - strand at 387.305 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 384,525 + Pf1N1B4_325 0.79 -1.3 384,525 + Pf1N1B4_325 0.79 +0.9 384,525 + Pf1N1B4_325 0.79 +2.7 384,525 + Pf1N1B4_325 0.79 +0.3 384,525 + Pf1N1B4_325 0.79 -0.1 384,525 + Pf1N1B4_325 0.79 -1.1 384,525 + Pf1N1B4_325 0.79 +1.7 384,525 + Pf1N1B4_325 0.79 -0.5 384,526 - Pf1N1B4_325 0.79 +1.2 384,526 - Pf1N1B4_325 0.79 -2.1 384,526 - Pf1N1B4_325 0.79 -1.7 384,533 + Pf1N1B4_325 0.79 +1.4 384,533 + Pf1N1B4_325 0.79 +0.5 384,560 + Pf1N1B4_325 0.82 +2.1 384,560 + Pf1N1B4_325 0.82 -1.7 384,560 + Pf1N1B4_325 0.82 -1.1 384,561 - Pf1N1B4_325 0.82 -2.4 384,561 - Pf1N1B4_325 0.82 -0.7 384,564 + Pf1N1B4_325 0.82 +0.9 384,565 - -0.1 384,565 - Pf1N1B4_325 0.82 -0.1 384,596 - Pf1N1B4_325 0.85 -0.2 384,663 - +0.4 384,695 + +1.1 384,696 - -0.5 384,696 - -0.1 384,696 - -2.4 384,847 + +0.9 384,850 - -1.1 385,061 + -0.1 385,062 - -2.4 385,196 + -0.1 385,587 + -1.4 385,590 - -1.3 385,787 - Pf1N1B4_327 0.30 -0.1 385,787 - Pf1N1B4_327 0.30 -2.4 385,904 - Pf1N1B4_327 0.42 -1.1 385,904 - Pf1N1B4_327 0.42 -1.7 386,938 + +0.6 386,939 - +1.6 386,940 + +1.4 387,303 + -0.4 387,304 - -0.1 387,305 + -2.7
Or see this region's nucleotide sequence