Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3246 and Pf1N1B4_3247 are separated by 3 nucleotides Pf1N1B4_3247 and Pf1N1B4_3248 are separated by 265 nucleotides
Pf1N1B4_3246 - RND efflux system, inner membrane transporter CmeB, at 3,633,755 to 3,636,916
_3246
Pf1N1B4_3247 - Multidrug efflux RND membrane fusion protein MexC, at 3,636,920 to 3,638,077
_3247
Pf1N1B4_3248 - Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family, at 3,638,343 to 3,638,975
_3248
Position (kb)
3636
3637
3638
3639 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 3636.791 kb on - strand at 3636.947 kb on - strand at 3636.947 kb on - strand at 3636.947 kb on - strand at 3636.950 kb on - strand at 3636.950 kb on - strand at 3636.950 kb on - strand at 3636.950 kb on - strand at 3636.950 kb on - strand at 3636.950 kb on - strand at 3637.492 kb on - strand, within Pf1N1B4_3247 at 3637.492 kb on - strand, within Pf1N1B4_3247 at 3637.600 kb on - strand at 3637.786 kb on - strand, within Pf1N1B4_3247 at 3638.154 kb on - strand at 3638.210 kb on - strand at 3638.249 kb on + strand at 3638.249 kb on + strand at 3638.299 kb on + strand at 3638.337 kb on - strand at 3638.415 kb on + strand, within Pf1N1B4_3248 at 3638.415 kb on + strand, within Pf1N1B4_3248 at 3638.416 kb on - strand, within Pf1N1B4_3248 at 3638.487 kb on + strand, within Pf1N1B4_3248 at 3638.487 kb on + strand, within Pf1N1B4_3248 at 3638.488 kb on - strand, within Pf1N1B4_3248 at 3638.504 kb on + strand, within Pf1N1B4_3248 at 3638.687 kb on + strand, within Pf1N1B4_3248 at 3638.687 kb on + strand, within Pf1N1B4_3248 at 3638.687 kb on + strand, within Pf1N1B4_3248 at 3638.688 kb on - strand, within Pf1N1B4_3248 at 3638.688 kb on - strand, within Pf1N1B4_3248 at 3638.850 kb on + strand, within Pf1N1B4_3248 at 3638.850 kb on + strand, within Pf1N1B4_3248 at 3638.851 kb on - strand, within Pf1N1B4_3248 at 3638.851 kb on - strand, within Pf1N1B4_3248 at 3638.942 kb on + strand at 3638.987 kb on + strand at 3638.988 kb on - strand at 3639.050 kb on + strand at 3639.050 kb on + strand at 3639.050 kb on + strand at 3639.051 kb on - strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.064 kb on + strand at 3639.065 kb on - strand at 3639.065 kb on - strand at 3639.065 kb on - strand at 3639.065 kb on - strand at 3639.065 kb on - strand at 3639.065 kb on - strand at 3639.066 kb on + strand at 3639.066 kb on + strand at 3639.066 kb on + strand at 3639.066 kb on + strand at 3639.066 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.068 kb on + strand at 3639.069 kb on - strand at 3639.069 kb on - strand at 3639.069 kb on - strand at 3639.069 kb on - strand at 3639.069 kb on - strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.072 kb on + strand at 3639.073 kb on - strand at 3639.073 kb on - strand at 3639.073 kb on - strand at 3639.073 kb on - strand at 3639.073 kb on - strand at 3639.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 3,636,791 - -1.9 3,636,947 - -3.1 3,636,947 - -2.7 3,636,947 - -1.5 3,636,950 - -2.2 3,636,950 - -2.5 3,636,950 - -1.9 3,636,950 - -3.8 3,636,950 - -0.3 3,636,950 - -1.5 3,637,492 - Pf1N1B4_3247 0.49 -1.5 3,637,492 - Pf1N1B4_3247 0.49 +0.7 3,637,600 - -1.5 3,637,786 - Pf1N1B4_3247 0.75 -2.7 3,638,154 - +1.4 3,638,210 - +6.2 3,638,249 + -1.9 3,638,249 + +0.1 3,638,299 + -1.5 3,638,337 - +7.5 3,638,415 + Pf1N1B4_3248 0.11 -0.1 3,638,415 + Pf1N1B4_3248 0.11 +0.7 3,638,416 - Pf1N1B4_3248 0.12 +7.5 3,638,487 + Pf1N1B4_3248 0.23 +1.8 3,638,487 + Pf1N1B4_3248 0.23 +2.1 3,638,488 - Pf1N1B4_3248 0.23 +7.9 3,638,504 + Pf1N1B4_3248 0.25 +3.9 3,638,687 + Pf1N1B4_3248 0.54 -0.3 3,638,687 + Pf1N1B4_3248 0.54 +4.1 3,638,687 + Pf1N1B4_3248 0.54 -2.2 3,638,688 - Pf1N1B4_3248 0.55 +4.4 3,638,688 - Pf1N1B4_3248 0.55 +7.4 3,638,850 + Pf1N1B4_3248 0.80 +3.5 3,638,850 + Pf1N1B4_3248 0.80 +2.9 3,638,851 - Pf1N1B4_3248 0.80 +7.0 3,638,851 - Pf1N1B4_3248 0.80 +5.7 3,638,942 + +5.9 3,638,987 + -0.5 3,638,988 - -0.5 3,639,050 + -1.9 3,639,050 + +0.1 3,639,050 + -0.9 3,639,051 - -0.3 3,639,064 + -2.2 3,639,064 + -0.9 3,639,064 + -1.5 3,639,064 + -1.9 3,639,064 + -1.5 3,639,064 + -0.9 3,639,064 + -1.9 3,639,064 + +1.1 3,639,065 - +0.1 3,639,065 - -1.9 3,639,065 - -0.9 3,639,065 - +1.1 3,639,065 - -3.2 3,639,065 - -2.9 3,639,066 + -2.2 3,639,066 + -1.5 3,639,066 + -1.5 3,639,066 + -1.5 3,639,066 + -1.5 3,639,068 + +0.1 3,639,068 + -2.5 3,639,068 + -3.1 3,639,068 + -1.5 3,639,068 + +0.1 3,639,068 + +0.4 3,639,068 + -1.5 3,639,068 + +0.1 3,639,068 + -1.9 3,639,068 + -1.5 3,639,068 + -1.9 3,639,068 + -3.2 3,639,068 + -1.5 3,639,068 + +0.1 3,639,068 + -2.5 3,639,068 + -0.9 3,639,068 + -1.9 3,639,068 + -1.9 3,639,069 - +0.1 3,639,069 - -0.9 3,639,069 - -1.5 3,639,069 - -1.5 3,639,069 - -3.6 3,639,072 + +0.1 3,639,072 + -2.7 3,639,072 + -0.9 3,639,072 + -0.9 3,639,072 + -2.2 3,639,072 + -2.2 3,639,072 + -0.9 3,639,072 + +0.1 3,639,072 + +1.1 3,639,072 + -2.2 3,639,072 + -1.5 3,639,073 - +0.1 3,639,073 - -0.9 3,639,073 - -1.5 3,639,073 - +1.1 3,639,073 - -0.9 3,639,073 - -0.9
Or see this region's nucleotide sequence