Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_321 and Pf1N1B4_322 are separated by 206 nucleotides Pf1N1B4_322 and Pf1N1B4_323 are separated by 299 nucleotides
Pf1N1B4_321 - Probable short-chain dehydrogenase, at 377,661 to 378,455
_321
Pf1N1B4_322 - nucleotide sugar epimerase/dehydratase WbpM, at 378,662 to 380,890
_322
Pf1N1B4_323 - Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-), at 381,190 to 382,218
_323
Position (kb) 378
379
380
381 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 377.669 kb on - strand at 377.691 kb on - strand at 377.691 kb on - strand at 377.691 kb on - strand at 377.726 kb on + strand at 377.726 kb on + strand at 377.727 kb on - strand at 377.885 kb on + strand, within Pf1N1B4_321 at 377.886 kb on - strand, within Pf1N1B4_321 at 377.971 kb on + strand at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand at 377.989 kb on + strand, within Pf1N1B4_321 at 377.990 kb on - strand, within Pf1N1B4_321 at 377.990 kb on - strand, within Pf1N1B4_321 at 377.990 kb on - strand, within Pf1N1B4_321 at 378.039 kb on - strand, within Pf1N1B4_321 at 378.186 kb on - strand, within Pf1N1B4_321 at 378.539 kb on + strand at 378.540 kb on - strand at 378.540 kb on - strand at 378.553 kb on + strand at 378.554 kb on - strand at 378.554 kb on - strand at 378.558 kb on + strand at 378.565 kb on - strand at 378.613 kb on - strand at 378.613 kb on - strand at 378.656 kb on - strand at 378.656 kb on - strand at 378.656 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.700 kb on + strand at 378.701 kb on - strand at 378.944 kb on - strand, within Pf1N1B4_322 at 378.979 kb on - strand, within Pf1N1B4_322 at 378.979 kb on - strand at 378.979 kb on - strand, within Pf1N1B4_322 at 378.998 kb on - strand, within Pf1N1B4_322 at 378.998 kb on - strand, within Pf1N1B4_322 at 379.693 kb on + strand, within Pf1N1B4_322 at 379.948 kb on - strand, within Pf1N1B4_322 at 380.481 kb on + strand at 380.481 kb on + strand, within Pf1N1B4_322 at 380.501 kb on - strand, within Pf1N1B4_322 at 380.742 kb on + strand at 380.918 kb on + strand at 380.967 kb on + strand at 380.968 kb on - strand at 380.968 kb on - strand at 381.040 kb on + strand at 381.160 kb on - strand at 381.242 kb on - strand at 381.260 kb on + strand at 381.260 kb on + strand at 381.395 kb on + strand, within Pf1N1B4_323 at 381.695 kb on + strand, within Pf1N1B4_323
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 377,669 - -0.5 377,691 - -0.1 377,691 - -0.8 377,691 - +2.5 377,726 + +1.4 377,726 + +0.2 377,727 - -0.5 377,885 + Pf1N1B4_321 0.28 -2.2 377,886 - Pf1N1B4_321 0.28 -0.1 377,971 + -0.1 377,972 - Pf1N1B4_321 0.39 -0.8 377,972 - Pf1N1B4_321 0.39 -0.1 377,972 - Pf1N1B4_321 0.39 -0.9 377,972 - Pf1N1B4_321 0.39 -0.4 377,972 - Pf1N1B4_321 0.39 +0.3 377,972 - Pf1N1B4_321 0.39 +1.3 377,972 - Pf1N1B4_321 0.39 -0.1 377,972 - Pf1N1B4_321 0.39 -0.7 377,972 - Pf1N1B4_321 0.39 +0.0 377,972 - Pf1N1B4_321 0.39 -0.3 377,972 - Pf1N1B4_321 0.39 +1.1 377,972 - Pf1N1B4_321 0.39 +0.2 377,972 - Pf1N1B4_321 0.39 -0.1 377,972 - +1.2 377,989 + Pf1N1B4_321 0.41 +0.7 377,990 - Pf1N1B4_321 0.41 -3.4 377,990 - Pf1N1B4_321 0.41 +1.2 377,990 - Pf1N1B4_321 0.41 +1.7 378,039 - Pf1N1B4_321 0.48 +1.1 378,186 - Pf1N1B4_321 0.66 -0.6 378,539 + +0.4 378,540 - -2.1 378,540 - +0.9 378,553 + +2.5 378,554 - +1.1 378,554 - +0.9 378,558 + +0.5 378,565 - -0.4 378,613 - +0.6 378,613 - +0.5 378,656 - +0.5 378,656 - -0.1 378,656 - +2.2 378,664 - -1.1 378,664 - +3.5 378,664 - +1.9 378,664 - -1.1 378,664 - +1.5 378,664 - +0.9 378,664 - +0.6 378,664 - -1.1 378,700 + -1.3 378,701 - +0.4 378,944 - Pf1N1B4_322 0.13 -1.1 378,979 - Pf1N1B4_322 0.14 -0.7 378,979 - -0.1 378,979 - Pf1N1B4_322 0.14 +0.1 378,998 - Pf1N1B4_322 0.15 -1.7 378,998 - Pf1N1B4_322 0.15 -1.7 379,693 + Pf1N1B4_322 0.46 -1.7 379,948 - Pf1N1B4_322 0.58 -1.1 380,481 + +1.7 380,481 + Pf1N1B4_322 0.82 -1.1 380,501 - Pf1N1B4_322 0.83 -1.1 380,742 + -0.5 380,918 + +2.2 380,967 + -3.1 380,968 - +4.8 380,968 - +0.8 381,040 + -2.4 381,160 - -1.7 381,242 - +0.3 381,260 + -1.4 381,260 + +0.9 381,395 + Pf1N1B4_323 0.20 -2.1 381,695 + Pf1N1B4_323 0.49 +1.2
Or see this region's nucleotide sequence