Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2782 and Pf1N1B4_2783 are separated by 40 nucleotides Pf1N1B4_2783 and Pf1N1B4_2784 are separated by 70 nucleotides Pf1N1B4_2784 and Pf1N1B4_2785 are separated by 205 nucleotides
Pf1N1B4_2782 - Mobile element protein, at 3,126,989 to 3,128,131
_2782
Pf1N1B4_2783 - Transcription elongation factor GreB-related protein, at 3,128,172 to 3,128,666
_2783
Pf1N1B4_2784 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,128,737 to 3,129,093
_2784
Pf1N1B4_2785 - putative outermembrane protein, at 3,129,299 to 3,131,152
_2785
Position (kb)
3128
3129 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3127.796 kb on + strand, within Pf1N1B4_2782 at 3128.129 kb on + strand at 3128.129 kb on + strand at 3128.129 kb on + strand at 3128.138 kb on + strand at 3128.148 kb on + strand at 3128.148 kb on + strand at 3128.449 kb on + strand, within Pf1N1B4_2783 at 3128.581 kb on + strand, within Pf1N1B4_2783 at 3128.582 kb on - strand, within Pf1N1B4_2783 at 3128.582 kb on - strand, within Pf1N1B4_2783 at 3128.582 kb on - strand, within Pf1N1B4_2783 at 3128.639 kb on + strand at 3128.640 kb on - strand at 3128.682 kb on + strand at 3128.682 kb on + strand at 3128.682 kb on + strand at 3128.683 kb on - strand at 3128.683 kb on - strand at 3128.683 kb on - strand at 3128.683 kb on - strand at 3128.683 kb on - strand at 3128.710 kb on - strand at 3128.710 kb on - strand at 3128.710 kb on - strand at 3128.729 kb on + strand at 3128.738 kb on + strand at 3128.738 kb on + strand at 3128.738 kb on + strand at 3128.738 kb on + strand at 3128.822 kb on + strand, within Pf1N1B4_2784 at 3128.822 kb on + strand, within Pf1N1B4_2784 at 3128.823 kb on - strand, within Pf1N1B4_2784 at 3128.823 kb on - strand, within Pf1N1B4_2784 at 3128.892 kb on - strand, within Pf1N1B4_2784 at 3128.892 kb on - strand, within Pf1N1B4_2784 at 3128.910 kb on - strand, within Pf1N1B4_2784 at 3129.123 kb on + strand at 3129.134 kb on + strand at 3129.134 kb on + strand at 3129.135 kb on - strand at 3129.135 kb on - strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.155 kb on + strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.156 kb on - strand at 3129.174 kb on + strand at 3129.175 kb on - strand at 3129.303 kb on + strand at 3129.303 kb on + strand at 3129.309 kb on + strand at 3129.310 kb on - strand at 3129.326 kb on + strand at 3129.326 kb on + strand at 3129.326 kb on + strand at 3129.327 kb on - strand at 3129.327 kb on - strand at 3129.327 kb on - strand at 3129.327 kb on - strand at 3129.327 kb on - strand at 3129.456 kb on + strand at 3129.456 kb on + strand at 3129.457 kb on - strand at 3129.457 kb on - strand at 3129.457 kb on - strand at 3129.457 kb on - strand at 3129.457 kb on - strand at 3129.457 kb on - strand at 3129.457 kb on - strand at 3129.503 kb on + strand, within Pf1N1B4_2785 at 3129.504 kb on - strand, within Pf1N1B4_2785 at 3129.559 kb on + strand, within Pf1N1B4_2785 at 3129.559 kb on + strand, within Pf1N1B4_2785 at 3129.559 kb on + strand, within Pf1N1B4_2785 at 3129.560 kb on - strand, within Pf1N1B4_2785 at 3129.560 kb on - strand, within Pf1N1B4_2785 at 3129.598 kb on + strand, within Pf1N1B4_2785 at 3129.599 kb on - strand at 3129.599 kb on - strand, within Pf1N1B4_2785
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 3,127,796 + Pf1N1B4_2782 0.71 -0.7 3,128,129 + -2.0 3,128,129 + +0.3 3,128,129 + -0.7 3,128,138 + +2.3 3,128,148 + -0.7 3,128,148 + +0.3 3,128,449 + Pf1N1B4_2783 0.56 -0.5 3,128,581 + Pf1N1B4_2783 0.83 -2.3 3,128,582 - Pf1N1B4_2783 0.83 -2.9 3,128,582 - Pf1N1B4_2783 0.83 -1.0 3,128,582 - Pf1N1B4_2783 0.83 +0.3 3,128,639 + -2.0 3,128,640 - +0.3 3,128,682 + -1.7 3,128,682 + +0.3 3,128,682 + +0.3 3,128,683 - +1.0 3,128,683 - -0.8 3,128,683 - +1.9 3,128,683 - +1.8 3,128,683 - +0.8 3,128,710 - +0.2 3,128,710 - -0.7 3,128,710 - +0.3 3,128,729 + -0.7 3,128,738 + -0.1 3,128,738 + +1.9 3,128,738 + -2.5 3,128,738 + +0.3 3,128,822 + Pf1N1B4_2784 0.24 +2.1 3,128,822 + Pf1N1B4_2784 0.24 +0.9 3,128,823 - Pf1N1B4_2784 0.24 +0.3 3,128,823 - Pf1N1B4_2784 0.24 +1.6 3,128,892 - Pf1N1B4_2784 0.43 -0.7 3,128,892 - Pf1N1B4_2784 0.43 -2.3 3,128,910 - Pf1N1B4_2784 0.48 +0.5 3,129,123 + +0.9 3,129,134 + +1.3 3,129,134 + -0.7 3,129,135 - +2.3 3,129,135 - +0.1 3,129,155 + +0.7 3,129,155 + +0.3 3,129,155 + -0.8 3,129,155 + -1.4 3,129,155 + -2.7 3,129,155 + -1.3 3,129,155 + +0.3 3,129,155 + -0.7 3,129,155 + -2.0 3,129,155 + +0.3 3,129,155 + +1.9 3,129,156 - -2.0 3,129,156 - -0.7 3,129,156 - -0.3 3,129,156 - -0.4 3,129,156 - +2.2 3,129,156 - -0.4 3,129,156 - -0.7 3,129,156 - -0.7 3,129,174 + +0.6 3,129,175 - +0.6 3,129,303 + -1.7 3,129,303 + +0.0 3,129,309 + -0.7 3,129,310 - -1.7 3,129,326 + +0.3 3,129,326 + -1.3 3,129,326 + +0.3 3,129,327 - -1.0 3,129,327 - -2.7 3,129,327 - -0.5 3,129,327 - +1.3 3,129,327 - -2.3 3,129,456 + -1.3 3,129,456 + -0.7 3,129,457 - -1.7 3,129,457 - +1.2 3,129,457 - +1.2 3,129,457 - +1.3 3,129,457 - -1.3 3,129,457 - +0.3 3,129,457 - +1.0 3,129,503 + Pf1N1B4_2785 0.11 +0.7 3,129,504 - Pf1N1B4_2785 0.11 +0.6 3,129,559 + Pf1N1B4_2785 0.14 +0.3 3,129,559 + Pf1N1B4_2785 0.14 -0.7 3,129,559 + Pf1N1B4_2785 0.14 +0.3 3,129,560 - Pf1N1B4_2785 0.14 -0.7 3,129,560 - Pf1N1B4_2785 0.14 -0.1 3,129,598 + Pf1N1B4_2785 0.16 -0.7 3,129,599 - -1.3 3,129,599 - Pf1N1B4_2785 0.16 +0.3
Or see this region's nucleotide sequence