Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2567 and Pf1N1B4_5198 are separated by 142 nucleotides Pf1N1B4_5198 and Pf1N1B4_5199 are separated by 27 nucleotides Pf1N1B4_5199 and Pf1N1B4_5200 are separated by 29 nucleotides Pf1N1B4_5200 and Pf1N1B4_2568 are separated by 87 nucleotides Pf1N1B4_2568 and Pf1N1B4_5201 are separated by 53 nucleotides Pf1N1B4_5201 and Pf1N1B4_2569 are separated by 48 nucleotides Pf1N1B4_2569 and Pf1N1B4_2570 are separated by 63 nucleotides
Pf1N1B4_2567 - FIG00956941: hypothetical protein, at 2,914,089 to 2,914,529
_2567
Pf1N1B4_5198 - tRNA-Tyr-GTA, at 2,914,672 to 2,914,753
_5198
Pf1N1B4_5199 - tRNA-Gly-TCC, at 2,914,781 to 2,914,851
_5199
Pf1N1B4_5200 - tRNA-Thr-GGT, at 2,914,881 to 2,914,953
_5200
Pf1N1B4_2568 - Translation elongation factor Tu, at 2,915,041 to 2,916,234
_2568
Pf1N1B4_5201 - tRNA-Trp-CCA, at 2,916,288 to 2,916,360
_5201
Pf1N1B4_2569 - Preprotein translocase subunit SecE (TC 3.A.5.1.1), at 2,916,409 to 2,916,777
_2569
Pf1N1B4_2570 - Transcription antitermination protein NusG, at 2,916,841 to 2,917,320
_2570
Position (kb)
2915
2916
2917 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2914.089 kb on - strand at 2914.131 kb on + strand at 2914.131 kb on + strand at 2914.131 kb on + strand at 2914.131 kb on + strand at 2914.132 kb on - strand at 2914.132 kb on - strand at 2914.132 kb on - strand at 2914.134 kb on + strand, within Pf1N1B4_2567 at 2914.134 kb on + strand, within Pf1N1B4_2567 at 2914.134 kb on + strand, within Pf1N1B4_2567 at 2914.134 kb on + strand, within Pf1N1B4_2567 at 2914.134 kb on + strand, within Pf1N1B4_2567 at 2914.134 kb on + strand, within Pf1N1B4_2567 at 2914.134 kb on + strand, within Pf1N1B4_2567 at 2914.134 kb on + strand, within Pf1N1B4_2567 at 2914.134 kb on + strand, within Pf1N1B4_2567 at 2914.135 kb on - strand, within Pf1N1B4_2567 at 2914.135 kb on - strand, within Pf1N1B4_2567 at 2914.135 kb on - strand, within Pf1N1B4_2567 at 2914.177 kb on + strand, within Pf1N1B4_2567 at 2914.177 kb on + strand, within Pf1N1B4_2567 at 2914.178 kb on - strand, within Pf1N1B4_2567 at 2914.178 kb on - strand, within Pf1N1B4_2567 at 2914.195 kb on - strand, within Pf1N1B4_2567 at 2914.195 kb on - strand, within Pf1N1B4_2567 at 2914.290 kb on + strand, within Pf1N1B4_2567 at 2914.290 kb on + strand, within Pf1N1B4_2567 at 2914.291 kb on - strand, within Pf1N1B4_2567 at 2914.392 kb on + strand, within Pf1N1B4_2567 at 2914.392 kb on + strand, within Pf1N1B4_2567 at 2914.393 kb on - strand, within Pf1N1B4_2567 at 2914.414 kb on + strand, within Pf1N1B4_2567 at 2914.414 kb on + strand, within Pf1N1B4_2567 at 2914.483 kb on + strand, within Pf1N1B4_2567 at 2914.483 kb on + strand, within Pf1N1B4_2567 at 2914.484 kb on - strand, within Pf1N1B4_2567 at 2914.495 kb on + strand at 2914.495 kb on + strand at 2914.495 kb on + strand at 2914.496 kb on - strand at 2914.496 kb on - strand at 2914.527 kb on + strand at 2914.527 kb on + strand at 2914.536 kb on + strand at 2914.536 kb on + strand at 2914.536 kb on + strand at 2914.536 kb on + strand at 2914.536 kb on + strand at 2914.603 kb on + strand at 2914.957 kb on + strand at 2914.957 kb on + strand at 2914.957 kb on + strand at 2914.957 kb on + strand at 2914.957 kb on + strand at 2914.959 kb on + strand at 2914.959 kb on + strand at 2914.959 kb on + strand at 2914.959 kb on + strand at 2914.959 kb on + strand at 2914.959 kb on + strand at 2914.959 kb on + strand at 2914.959 kb on + strand at 2914.959 kb on + strand at 2914.959 kb on + strand at 2914.959 kb on + strand at 2914.959 kb on + strand at 2914.959 kb on + strand at 2914.959 kb on + strand at 2914.971 kb on + strand at 2914.979 kb on + strand at 2914.979 kb on + strand at 2914.979 kb on + strand at 2914.979 kb on + strand at 2914.979 kb on + strand at 2914.994 kb on + strand at 2914.994 kb on + strand at 2915.004 kb on + strand at 2915.007 kb on + strand at 2915.007 kb on + strand at 2915.007 kb on + strand at 2915.007 kb on + strand at 2915.015 kb on + strand at 2915.015 kb on + strand at 2915.015 kb on + strand at 2915.015 kb on + strand at 2915.015 kb on + strand at 2915.015 kb on + strand at 2915.269 kb on + strand, within Pf1N1B4_2568 at 2915.306 kb on + strand, within Pf1N1B4_2568 at 2915.428 kb on + strand, within Pf1N1B4_2568 at 2915.521 kb on + strand, within Pf1N1B4_2568 at 2915.521 kb on + strand, within Pf1N1B4_2568 at 2915.644 kb on + strand, within Pf1N1B4_2568 at 2915.811 kb on + strand, within Pf1N1B4_2568 at 2915.811 kb on + strand, within Pf1N1B4_2568 at 2916.043 kb on + strand, within Pf1N1B4_2568 at 2916.043 kb on + strand, within Pf1N1B4_2568 at 2916.043 kb on + strand at 2916.051 kb on + strand, within Pf1N1B4_2568 at 2916.114 kb on + strand, within Pf1N1B4_2568 at 2916.215 kb on + strand at 2916.215 kb on + strand at 2916.232 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 2,914,089 - +0.4 2,914,131 + -2.4 2,914,131 + +0.1 2,914,131 + -0.0 2,914,131 + -1.0 2,914,132 - -0.6 2,914,132 - -0.6 2,914,132 - +0.4 2,914,134 + Pf1N1B4_2567 0.10 +1.0 2,914,134 + Pf1N1B4_2567 0.10 +2.7 2,914,134 + Pf1N1B4_2567 0.10 +1.0 2,914,134 + Pf1N1B4_2567 0.10 -1.2 2,914,134 + Pf1N1B4_2567 0.10 -1.6 2,914,134 + Pf1N1B4_2567 0.10 -0.2 2,914,134 + Pf1N1B4_2567 0.10 -1.2 2,914,134 + Pf1N1B4_2567 0.10 -1.3 2,914,134 + Pf1N1B4_2567 0.10 -0.0 2,914,135 - Pf1N1B4_2567 0.10 -1.2 2,914,135 - Pf1N1B4_2567 0.10 -0.0 2,914,135 - Pf1N1B4_2567 0.10 -1.9 2,914,177 + Pf1N1B4_2567 0.20 -0.8 2,914,177 + Pf1N1B4_2567 0.20 -0.8 2,914,178 - Pf1N1B4_2567 0.20 -0.0 2,914,178 - Pf1N1B4_2567 0.20 -1.6 2,914,195 - Pf1N1B4_2567 0.24 +1.1 2,914,195 - Pf1N1B4_2567 0.24 +1.8 2,914,290 + Pf1N1B4_2567 0.46 +0.1 2,914,290 + Pf1N1B4_2567 0.46 -0.6 2,914,291 - Pf1N1B4_2567 0.46 +0.7 2,914,392 + Pf1N1B4_2567 0.69 +2.4 2,914,392 + Pf1N1B4_2567 0.69 +0.9 2,914,393 - Pf1N1B4_2567 0.69 +2.4 2,914,414 + Pf1N1B4_2567 0.74 -1.6 2,914,414 + Pf1N1B4_2567 0.74 -0.4 2,914,483 + Pf1N1B4_2567 0.89 +1.5 2,914,483 + Pf1N1B4_2567 0.89 -0.4 2,914,484 - Pf1N1B4_2567 0.90 +1.2 2,914,495 + -2.4 2,914,495 + -2.4 2,914,495 + +1.5 2,914,496 - +0.8 2,914,496 - +0.4 2,914,527 + +2.2 2,914,527 + -1.2 2,914,536 + +2.2 2,914,536 + +2.6 2,914,536 + +1.8 2,914,536 + +2.0 2,914,536 + +3.8 2,914,603 + +1.2 2,914,957 + -1.6 2,914,957 + -0.2 2,914,957 + -1.2 2,914,957 + -0.6 2,914,957 + +3.2 2,914,959 + -1.6 2,914,959 + -0.6 2,914,959 + -0.7 2,914,959 + -2.2 2,914,959 + +0.4 2,914,959 + -0.0 2,914,959 + +2.4 2,914,959 + -0.9 2,914,959 + -0.2 2,914,959 + -0.2 2,914,959 + -1.9 2,914,959 + -1.9 2,914,959 + -0.6 2,914,959 + +2.7 2,914,971 + +0.0 2,914,979 + +0.4 2,914,979 + +1.4 2,914,979 + +0.4 2,914,979 + -1.9 2,914,979 + +0.7 2,914,994 + +0.4 2,914,994 + +0.4 2,915,004 + +2.4 2,915,007 + -0.9 2,915,007 + +0.4 2,915,007 + +0.4 2,915,007 + -1.6 2,915,015 + -1.6 2,915,015 + +0.4 2,915,015 + -1.9 2,915,015 + +2.4 2,915,015 + -1.2 2,915,015 + +0.4 2,915,269 + Pf1N1B4_2568 0.19 +0.4 2,915,306 + Pf1N1B4_2568 0.22 -1.9 2,915,428 + Pf1N1B4_2568 0.32 -1.2 2,915,521 + Pf1N1B4_2568 0.40 -1.6 2,915,521 + Pf1N1B4_2568 0.40 -1.9 2,915,644 + Pf1N1B4_2568 0.51 -1.6 2,915,811 + Pf1N1B4_2568 0.64 +0.8 2,915,811 + Pf1N1B4_2568 0.64 -1.6 2,916,043 + Pf1N1B4_2568 0.84 -2.4 2,916,043 + Pf1N1B4_2568 0.84 -1.2 2,916,043 + -1.9 2,916,051 + Pf1N1B4_2568 0.85 -1.2 2,916,114 + Pf1N1B4_2568 0.90 +0.8 2,916,215 + -2.2 2,916,215 + -1.6 2,916,232 + -0.6
Or see this region's nucleotide sequence