Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2476 and Pf1N1B4_2477 are separated by 266 nucleotides Pf1N1B4_2477 and Pf1N1B4_2478 are separated by 173 nucleotides Pf1N1B4_2478 and Pf1N1B4_2479 are separated by 609 nucleotides
Pf1N1B4_2476 - Predicted signal-transduction protein containing cAMP-binding and CBS domains, at 2,807,554 to 2,809,494
_2476
Pf1N1B4_2477 - response regulator, at 2,809,761 to 2,810,213
_2477
Pf1N1B4_2478 - Malate synthase G (EC 2.3.3.9), at 2,810,387 to 2,812,564
_2478
Pf1N1B4_2479 - Glycine cleavage system transcriptional antiactivator GcvR, at 2,813,174 to 2,813,692
_2479
Position (kb)
2810
2811
2812
2813 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2809.411 kb on + strand at 2809.412 kb on - strand at 2809.412 kb on - strand at 2809.412 kb on - strand at 2809.489 kb on - strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.593 kb on + strand at 2809.593 kb on + strand at 2809.593 kb on + strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.597 kb on + strand at 2809.598 kb on - strand at 2809.598 kb on - strand at 2809.598 kb on - strand at 2809.619 kb on - strand at 2809.656 kb on + strand at 2809.656 kb on + strand at 2809.657 kb on - strand at 2809.657 kb on - strand at 2809.775 kb on + strand at 2809.775 kb on + strand at 2809.776 kb on - strand at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.129 kb on - strand, within Pf1N1B4_2477 at 2810.385 kb on - strand at 2810.657 kb on - strand, within Pf1N1B4_2478 at 2810.657 kb on - strand at 2810.657 kb on - strand, within Pf1N1B4_2478 at 2810.749 kb on - strand, within Pf1N1B4_2478 at 2810.907 kb on + strand, within Pf1N1B4_2478 at 2810.972 kb on - strand, within Pf1N1B4_2478 at 2811.296 kb on - strand at 2811.388 kb on + strand, within Pf1N1B4_2478 at 2811.388 kb on + strand, within Pf1N1B4_2478 at 2811.391 kb on - strand, within Pf1N1B4_2478 at 2811.391 kb on - strand, within Pf1N1B4_2478 at 2811.391 kb on - strand, within Pf1N1B4_2478 at 2811.391 kb on - strand, within Pf1N1B4_2478 at 2811.439 kb on + strand, within Pf1N1B4_2478 at 2811.440 kb on - strand, within Pf1N1B4_2478 at 2811.479 kb on - strand, within Pf1N1B4_2478 at 2811.489 kb on - strand, within Pf1N1B4_2478 at 2811.506 kb on - strand, within Pf1N1B4_2478 at 2811.601 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.603 kb on + strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.604 kb on - strand, within Pf1N1B4_2478 at 2811.699 kb on + strand, within Pf1N1B4_2478 at 2811.700 kb on - strand, within Pf1N1B4_2478 at 2811.987 kb on + strand, within Pf1N1B4_2478 at 2811.987 kb on + strand, within Pf1N1B4_2478 at 2811.988 kb on - strand, within Pf1N1B4_2478 at 2811.988 kb on - strand, within Pf1N1B4_2478 at 2812.086 kb on + strand, within Pf1N1B4_2478 at 2812.087 kb on - strand, within Pf1N1B4_2478 at 2812.137 kb on + strand, within Pf1N1B4_2478 at 2812.137 kb on + strand, within Pf1N1B4_2478 at 2812.138 kb on - strand, within Pf1N1B4_2478 at 2812.149 kb on + strand, within Pf1N1B4_2478 at 2812.149 kb on + strand, within Pf1N1B4_2478 at 2812.323 kb on + strand, within Pf1N1B4_2478 at 2812.323 kb on + strand, within Pf1N1B4_2478 at 2812.323 kb on + strand, within Pf1N1B4_2478 at 2812.375 kb on + strand at 2812.376 kb on - strand at 2812.407 kb on - strand at 2812.635 kb on + strand at 2812.635 kb on + strand at 2812.636 kb on - strand at 2812.636 kb on - strand at 2812.652 kb on + strand at 2812.653 kb on - strand at 2812.653 kb on - strand at 2812.653 kb on - strand at 2812.658 kb on + strand at 2812.658 kb on + strand at 2812.658 kb on + strand at 2812.659 kb on - strand at 2812.659 kb on - strand at 2812.659 kb on - strand at 2812.767 kb on + strand at 2812.767 kb on + strand at 2812.768 kb on - strand at 2812.768 kb on - strand at 2812.771 kb on + strand at 2812.771 kb on + strand at 2812.772 kb on - strand at 2812.788 kb on + strand at 2812.789 kb on - strand at 2812.791 kb on + strand at 2812.792 kb on - strand at 2812.792 kb on - strand at 2812.823 kb on + strand at 2812.823 kb on + strand at 2812.823 kb on + strand at 2812.824 kb on - strand at 2812.824 kb on - strand at 2812.827 kb on + strand at 2812.827 kb on + strand at 2812.827 kb on + strand at 2812.828 kb on - strand at 2812.828 kb on - strand at 2812.925 kb on + strand at 2812.925 kb on + strand at 2812.926 kb on - strand at 2812.931 kb on + strand at 2812.931 kb on + strand at 2812.931 kb on + strand at 2812.931 kb on + strand at 2812.932 kb on - strand at 2813.017 kb on - strand at 2813.128 kb on + strand at 2813.128 kb on + strand at 2813.128 kb on + strand at 2813.128 kb on + strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.143 kb on - strand at 2813.143 kb on - strand at 2813.188 kb on - strand at 2813.339 kb on + strand, within Pf1N1B4_2479 at 2813.352 kb on + strand, within Pf1N1B4_2479 at 2813.353 kb on - strand, within Pf1N1B4_2479 at 2813.541 kb on + strand, within Pf1N1B4_2479 at 2813.541 kb on + strand, within Pf1N1B4_2479 at 2813.542 kb on - strand, within Pf1N1B4_2479
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 2,809,411 + +1.2 2,809,412 - -2.0 2,809,412 - -1.2 2,809,412 - -0.6 2,809,489 - +0.7 2,809,587 + -0.0 2,809,587 + +1.0 2,809,587 + +0.6 2,809,587 + +2.4 2,809,587 + -3.1 2,809,587 + -0.6 2,809,587 + +0.1 2,809,587 + -1.2 2,809,588 - -1.2 2,809,588 - +3.4 2,809,588 - -0.8 2,809,588 - -0.1 2,809,588 - -1.4 2,809,593 + -1.0 2,809,593 + -1.6 2,809,593 + +0.4 2,809,594 - -3.0 2,809,594 - +1.4 2,809,594 - -0.6 2,809,594 - +1.5 2,809,594 - -0.2 2,809,594 - -1.6 2,809,594 - -0.2 2,809,594 - -0.6 2,809,594 - +1.4 2,809,597 + -1.5 2,809,598 - -0.7 2,809,598 - -0.6 2,809,598 - -1.6 2,809,619 - -0.6 2,809,656 + -1.6 2,809,656 + -2.8 2,809,657 - -0.3 2,809,657 - -1.2 2,809,775 + +1.2 2,809,775 + -1.0 2,809,776 - +0.8 2,809,954 + Pf1N1B4_2477 0.43 -1.6 2,809,954 + Pf1N1B4_2477 0.43 +1.4 2,809,954 + Pf1N1B4_2477 0.43 -3.2 2,809,954 + Pf1N1B4_2477 0.43 +2.0 2,810,128 + Pf1N1B4_2477 0.81 +1.1 2,810,128 + Pf1N1B4_2477 0.81 +0.4 2,810,128 + Pf1N1B4_2477 0.81 -0.4 2,810,129 - Pf1N1B4_2477 0.81 -0.6 2,810,385 - -1.6 2,810,657 - Pf1N1B4_2478 0.12 -0.6 2,810,657 - +1.0 2,810,657 - Pf1N1B4_2478 0.12 +2.0 2,810,749 - Pf1N1B4_2478 0.17 +2.4 2,810,907 + Pf1N1B4_2478 0.24 +0.0 2,810,972 - Pf1N1B4_2478 0.27 +0.3 2,811,296 - +0.4 2,811,388 + Pf1N1B4_2478 0.46 +1.6 2,811,388 + Pf1N1B4_2478 0.46 +1.8 2,811,391 - Pf1N1B4_2478 0.46 -0.6 2,811,391 - Pf1N1B4_2478 0.46 -0.4 2,811,391 - Pf1N1B4_2478 0.46 -0.9 2,811,391 - Pf1N1B4_2478 0.46 +1.4 2,811,439 + Pf1N1B4_2478 0.48 +0.4 2,811,440 - Pf1N1B4_2478 0.48 -1.2 2,811,479 - Pf1N1B4_2478 0.50 -0.6 2,811,489 - Pf1N1B4_2478 0.51 -0.4 2,811,506 - Pf1N1B4_2478 0.51 +1.7 2,811,601 + Pf1N1B4_2478 0.56 -1.6 2,811,603 + Pf1N1B4_2478 0.56 -1.4 2,811,603 + Pf1N1B4_2478 0.56 -0.6 2,811,603 + Pf1N1B4_2478 0.56 -2.0 2,811,603 + Pf1N1B4_2478 0.56 +2.7 2,811,603 + Pf1N1B4_2478 0.56 -0.4 2,811,603 + Pf1N1B4_2478 0.56 -0.8 2,811,603 + Pf1N1B4_2478 0.56 -1.6 2,811,604 - Pf1N1B4_2478 0.56 -0.4 2,811,604 - Pf1N1B4_2478 0.56 -0.6 2,811,604 - Pf1N1B4_2478 0.56 -1.0 2,811,604 - Pf1N1B4_2478 0.56 -1.2 2,811,604 - Pf1N1B4_2478 0.56 -0.2 2,811,604 - Pf1N1B4_2478 0.56 -1.9 2,811,604 - Pf1N1B4_2478 0.56 -3.2 2,811,604 - Pf1N1B4_2478 0.56 +2.6 2,811,699 + Pf1N1B4_2478 0.60 -2.0 2,811,700 - Pf1N1B4_2478 0.60 +0.6 2,811,987 + Pf1N1B4_2478 0.73 +0.4 2,811,987 + Pf1N1B4_2478 0.73 -2.0 2,811,988 - Pf1N1B4_2478 0.74 +1.4 2,811,988 - Pf1N1B4_2478 0.74 +0.4 2,812,086 + Pf1N1B4_2478 0.78 -0.4 2,812,087 - Pf1N1B4_2478 0.78 -0.6 2,812,137 + Pf1N1B4_2478 0.80 -1.6 2,812,137 + Pf1N1B4_2478 0.80 -1.8 2,812,138 - Pf1N1B4_2478 0.80 +2.0 2,812,149 + Pf1N1B4_2478 0.81 +0.4 2,812,149 + Pf1N1B4_2478 0.81 -1.6 2,812,323 + Pf1N1B4_2478 0.89 +0.8 2,812,323 + Pf1N1B4_2478 0.89 +1.4 2,812,323 + Pf1N1B4_2478 0.89 -2.1 2,812,375 + +0.0 2,812,376 - -2.4 2,812,407 - -2.8 2,812,635 + -1.8 2,812,635 + -2.2 2,812,636 - -1.6 2,812,636 - -0.5 2,812,652 + -1.2 2,812,653 - -2.2 2,812,653 - +0.4 2,812,653 - -2.2 2,812,658 + +0.8 2,812,658 + -2.0 2,812,658 + -1.2 2,812,659 - -0.2 2,812,659 - -0.3 2,812,659 - -1.6 2,812,767 + +0.8 2,812,767 + +0.0 2,812,768 - -0.6 2,812,768 - +0.4 2,812,771 + +0.4 2,812,771 + -2.4 2,812,772 - +0.1 2,812,788 + -0.2 2,812,789 - -1.2 2,812,791 + +1.0 2,812,792 - -1.6 2,812,792 - +0.4 2,812,823 + -1.2 2,812,823 + +2.0 2,812,823 + +2.4 2,812,824 - +0.8 2,812,824 - -0.4 2,812,827 + +1.4 2,812,827 + +0.6 2,812,827 + -1.2 2,812,828 - +1.7 2,812,828 - +0.4 2,812,925 + -0.0 2,812,925 + -1.2 2,812,926 - -2.0 2,812,931 + -1.8 2,812,931 + -0.6 2,812,931 + -1.2 2,812,931 + -2.2 2,812,932 - -1.0 2,813,017 - -0.8 2,813,128 + +2.0 2,813,128 + +0.4 2,813,128 + -2.0 2,813,128 + -1.2 2,813,129 - -0.6 2,813,129 - -0.5 2,813,129 - -0.6 2,813,129 - -2.4 2,813,129 - -2.2 2,813,129 - -2.6 2,813,129 - -2.0 2,813,129 - +0.4 2,813,130 + -1.2 2,813,130 + -0.4 2,813,130 + -0.2 2,813,130 + -0.9 2,813,130 + +0.7 2,813,130 + -0.4 2,813,130 + +0.1 2,813,130 + -0.2 2,813,130 + +2.0 2,813,131 - -1.2 2,813,131 - +0.5 2,813,131 - -0.6 2,813,131 - -1.0 2,813,131 - -2.1 2,813,131 - -1.6 2,813,131 - +0.2 2,813,131 - -0.6 2,813,143 - -1.3 2,813,143 - +0.4 2,813,188 - -0.2 2,813,339 + Pf1N1B4_2479 0.32 -1.6 2,813,352 + Pf1N1B4_2479 0.34 -1.6 2,813,353 - Pf1N1B4_2479 0.34 -1.2 2,813,541 + Pf1N1B4_2479 0.71 -0.1 2,813,541 + Pf1N1B4_2479 0.71 +0.4 2,813,542 - Pf1N1B4_2479 0.71 +1.5
Or see this region's nucleotide sequence