Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2461 and Pf1N1B4_2462 are separated by 240 nucleotides Pf1N1B4_2462 and Pf1N1B4_2463 are separated by 1 nucleotides
Pf1N1B4_2461 - Threonine dehydrogenase and related Zn-dependent dehydrogenases, at 2,791,510 to 2,792,709
_2461
Pf1N1B4_2462 - FIG00955037: hypothetical protein, at 2,792,950 to 2,793,567
_2462
Pf1N1B4_2463 - Acyltransferase family protein, at 2,793,569 to 2,794,453
_2463
Position (kb)
2792
2793
2794 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2792.080 kb on + strand, within Pf1N1B4_2461 at 2792.080 kb on + strand, within Pf1N1B4_2461 at 2792.081 kb on - strand at 2792.298 kb on - strand, within Pf1N1B4_2461 at 2792.416 kb on + strand, within Pf1N1B4_2461 at 2792.539 kb on + strand, within Pf1N1B4_2461 at 2792.539 kb on + strand, within Pf1N1B4_2461 at 2792.539 kb on + strand, within Pf1N1B4_2461 at 2792.540 kb on - strand, within Pf1N1B4_2461 at 2792.540 kb on - strand, within Pf1N1B4_2461 at 2792.540 kb on - strand, within Pf1N1B4_2461 at 2792.638 kb on + strand at 2792.707 kb on + strand at 2792.823 kb on + strand at 2792.841 kb on + strand at 2793.312 kb on + strand, within Pf1N1B4_2462 at 2793.478 kb on + strand, within Pf1N1B4_2462 at 2793.479 kb on - strand, within Pf1N1B4_2462 at 2793.479 kb on - strand, within Pf1N1B4_2462 at 2793.557 kb on + strand at 2793.557 kb on + strand at 2793.557 kb on + strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.561 kb on + strand at 2793.562 kb on - strand at 2793.562 kb on - strand at 2793.669 kb on + strand, within Pf1N1B4_2463 at 2793.912 kb on + strand, within Pf1N1B4_2463 at 2793.930 kb on + strand, within Pf1N1B4_2463 at 2793.931 kb on - strand, within Pf1N1B4_2463 at 2793.931 kb on - strand, within Pf1N1B4_2463 at 2793.931 kb on - strand, within Pf1N1B4_2463 at 2793.931 kb on - strand, within Pf1N1B4_2463 at 2793.960 kb on + strand, within Pf1N1B4_2463 at 2793.960 kb on + strand, within Pf1N1B4_2463 at 2793.960 kb on + strand, within Pf1N1B4_2463 at 2793.961 kb on - strand, within Pf1N1B4_2463 at 2793.961 kb on - strand, within Pf1N1B4_2463 at 2793.961 kb on - strand, within Pf1N1B4_2463 at 2793.984 kb on - strand, within Pf1N1B4_2463 at 2794.057 kb on - strand, within Pf1N1B4_2463 at 2794.057 kb on - strand, within Pf1N1B4_2463 at 2794.203 kb on + strand, within Pf1N1B4_2463 at 2794.203 kb on + strand, within Pf1N1B4_2463 at 2794.203 kb on + strand, within Pf1N1B4_2463 at 2794.203 kb on + strand at 2794.203 kb on + strand, within Pf1N1B4_2463 at 2794.204 kb on - strand, within Pf1N1B4_2463 at 2794.204 kb on - strand, within Pf1N1B4_2463 at 2794.204 kb on - strand, within Pf1N1B4_2463 at 2794.329 kb on + strand, within Pf1N1B4_2463 at 2794.329 kb on + strand, within Pf1N1B4_2463 at 2794.330 kb on - strand, within Pf1N1B4_2463 at 2794.453 kb on + strand at 2794.453 kb on + strand at 2794.453 kb on + strand at 2794.453 kb on + strand at 2794.453 kb on + strand at 2794.453 kb on + strand at 2794.454 kb on - strand at 2794.454 kb on - strand at 2794.454 kb on - strand at 2794.454 kb on - strand at 2794.488 kb on + strand at 2794.489 kb on - strand at 2794.489 kb on - strand at 2794.563 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 2,792,080 + Pf1N1B4_2461 0.47 -0.2 2,792,080 + Pf1N1B4_2461 0.47 +0.4 2,792,081 - +0.4 2,792,298 - Pf1N1B4_2461 0.66 -0.6 2,792,416 + Pf1N1B4_2461 0.76 -0.4 2,792,539 + Pf1N1B4_2461 0.86 -0.2 2,792,539 + Pf1N1B4_2461 0.86 +2.0 2,792,539 + Pf1N1B4_2461 0.86 -2.9 2,792,540 - Pf1N1B4_2461 0.86 -0.6 2,792,540 - Pf1N1B4_2461 0.86 -1.8 2,792,540 - Pf1N1B4_2461 0.86 -1.4 2,792,638 + -1.2 2,792,707 + -2.8 2,792,823 + +0.4 2,792,841 + -0.4 2,793,312 + Pf1N1B4_2462 0.59 -1.2 2,793,478 + Pf1N1B4_2462 0.85 +0.1 2,793,479 - Pf1N1B4_2462 0.86 -1.6 2,793,479 - Pf1N1B4_2462 0.86 +0.8 2,793,557 + +2.0 2,793,557 + -1.6 2,793,557 + -1.2 2,793,558 - -0.1 2,793,558 - -0.6 2,793,558 - -2.2 2,793,558 - +0.8 2,793,558 - -0.6 2,793,558 - -0.4 2,793,561 + -0.6 2,793,562 - +0.4 2,793,562 - +0.8 2,793,669 + Pf1N1B4_2463 0.11 +2.8 2,793,912 + Pf1N1B4_2463 0.39 +1.0 2,793,930 + Pf1N1B4_2463 0.41 +2.0 2,793,931 - Pf1N1B4_2463 0.41 +0.8 2,793,931 - Pf1N1B4_2463 0.41 -0.6 2,793,931 - Pf1N1B4_2463 0.41 +1.6 2,793,931 - Pf1N1B4_2463 0.41 +0.4 2,793,960 + Pf1N1B4_2463 0.44 +2.2 2,793,960 + Pf1N1B4_2463 0.44 -0.2 2,793,960 + Pf1N1B4_2463 0.44 -0.2 2,793,961 - Pf1N1B4_2463 0.44 -0.6 2,793,961 - Pf1N1B4_2463 0.44 +1.0 2,793,961 - Pf1N1B4_2463 0.44 -1.6 2,793,984 - Pf1N1B4_2463 0.47 -0.6 2,794,057 - Pf1N1B4_2463 0.55 +0.4 2,794,057 - Pf1N1B4_2463 0.55 +1.6 2,794,203 + Pf1N1B4_2463 0.72 -1.2 2,794,203 + Pf1N1B4_2463 0.72 +1.4 2,794,203 + Pf1N1B4_2463 0.72 +1.8 2,794,203 + -0.6 2,794,203 + Pf1N1B4_2463 0.72 +2.0 2,794,204 - Pf1N1B4_2463 0.72 +0.8 2,794,204 - Pf1N1B4_2463 0.72 +2.1 2,794,204 - Pf1N1B4_2463 0.72 +0.4 2,794,329 + Pf1N1B4_2463 0.86 +0.4 2,794,329 + Pf1N1B4_2463 0.86 +0.1 2,794,330 - Pf1N1B4_2463 0.86 -1.6 2,794,453 + +1.2 2,794,453 + -1.6 2,794,453 + -1.6 2,794,453 + -1.2 2,794,453 + +1.2 2,794,453 + +1.7 2,794,454 - +1.4 2,794,454 - -1.9 2,794,454 - +1.4 2,794,454 - +0.4 2,794,488 + -0.0 2,794,489 - -0.6 2,794,489 - -0.6 2,794,563 - -0.6
Or see this region's nucleotide sequence