Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_220 and Pf1N1B4_221 are separated by 12 nucleotides Pf1N1B4_221 and Pf1N1B4_222 are separated by 2 nucleotides Pf1N1B4_222 and Pf1N1B4_223 are separated by 134 nucleotides
Pf1N1B4_220 - 3-hydroxy-3isohexenylglutaryl-CoA:acetate lyase, at 279,272 to 281,074
_220
Pf1N1B4_221 - Citronellol and citronellal dehydrogenase, at 281,087 to 282,001
_221
Pf1N1B4_222 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 282,004 to 283,620
_222
Pf1N1B4_223 - Citronellyl-CoA dehydrogenase, at 283,755 to 284,912
_223
Position (kb) 282
283
284 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 281.073 kb on - strand at 281.073 kb on - strand at 281.073 kb on - strand at 281.073 kb on - strand at 281.073 kb on - strand at 281.093 kb on + strand at 281.093 kb on + strand at 281.394 kb on - strand, within Pf1N1B4_221 at 281.439 kb on + strand, within Pf1N1B4_221 at 281.439 kb on + strand, within Pf1N1B4_221 at 281.440 kb on - strand, within Pf1N1B4_221 at 281.693 kb on + strand, within Pf1N1B4_221 at 281.693 kb on + strand, within Pf1N1B4_221 at 281.693 kb on + strand at 281.693 kb on + strand, within Pf1N1B4_221 at 281.694 kb on - strand, within Pf1N1B4_221 at 281.694 kb on - strand, within Pf1N1B4_221 at 281.740 kb on - strand, within Pf1N1B4_221 at 281.740 kb on - strand, within Pf1N1B4_221 at 281.903 kb on + strand, within Pf1N1B4_221 at 281.904 kb on - strand, within Pf1N1B4_221 at 281.904 kb on - strand, within Pf1N1B4_221 at 281.924 kb on + strand at 281.925 kb on - strand at 281.925 kb on - strand at 281.928 kb on + strand at 281.937 kb on + strand at 281.938 kb on - strand at 282.181 kb on - strand, within Pf1N1B4_222 at 282.181 kb on - strand, within Pf1N1B4_222 at 282.463 kb on + strand, within Pf1N1B4_222 at 282.464 kb on - strand at 282.601 kb on + strand, within Pf1N1B4_222 at 282.602 kb on - strand, within Pf1N1B4_222 at 282.739 kb on + strand, within Pf1N1B4_222 at 282.739 kb on + strand, within Pf1N1B4_222 at 282.739 kb on + strand, within Pf1N1B4_222 at 283.224 kb on + strand, within Pf1N1B4_222 at 283.225 kb on - strand, within Pf1N1B4_222 at 283.252 kb on + strand, within Pf1N1B4_222 at 283.480 kb on + strand at 283.481 kb on - strand at 283.481 kb on - strand at 283.481 kb on - strand at 283.481 kb on - strand at 283.546 kb on + strand at 283.547 kb on - strand at 283.633 kb on - strand at 283.696 kb on + strand at 283.948 kb on - strand, within Pf1N1B4_223 at 283.948 kb on - strand, within Pf1N1B4_223 at 283.948 kb on - strand, within Pf1N1B4_223 at 283.954 kb on - strand, within Pf1N1B4_223 at 284.156 kb on - strand, within Pf1N1B4_223 at 284.196 kb on + strand, within Pf1N1B4_223 at 284.196 kb on + strand, within Pf1N1B4_223 at 284.197 kb on - strand at 284.197 kb on - strand, within Pf1N1B4_223 at 284.197 kb on - strand, within Pf1N1B4_223 at 284.332 kb on + strand, within Pf1N1B4_223 at 284.333 kb on - strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.521 kb on - strand, within Pf1N1B4_223 at 284.544 kb on + strand, within Pf1N1B4_223
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 281,073 - +0.8 281,073 - -1.8 281,073 - -0.2 281,073 - -1.8 281,073 - +1.6 281,093 + -0.2 281,093 + +0.4 281,394 - Pf1N1B4_221 0.34 +2.3 281,439 + Pf1N1B4_221 0.38 +0.6 281,439 + Pf1N1B4_221 0.38 -0.4 281,440 - Pf1N1B4_221 0.39 -3.4 281,693 + Pf1N1B4_221 0.66 -1.0 281,693 + Pf1N1B4_221 0.66 +0.2 281,693 + -0.2 281,693 + Pf1N1B4_221 0.66 -0.9 281,694 - Pf1N1B4_221 0.66 -2.8 281,694 - Pf1N1B4_221 0.66 +0.8 281,740 - Pf1N1B4_221 0.71 -0.2 281,740 - Pf1N1B4_221 0.71 -1.2 281,903 + Pf1N1B4_221 0.89 -1.8 281,904 - Pf1N1B4_221 0.89 -1.2 281,904 - Pf1N1B4_221 0.89 -1.2 281,924 + +0.0 281,925 - -1.8 281,925 - -1.2 281,928 + -0.2 281,937 + +0.4 281,938 - +2.8 282,181 - Pf1N1B4_222 0.11 -1.8 282,181 - Pf1N1B4_222 0.11 -1.2 282,463 + Pf1N1B4_222 0.28 -3.0 282,464 - -1.2 282,601 + Pf1N1B4_222 0.37 -0.2 282,602 - Pf1N1B4_222 0.37 -1.1 282,739 + Pf1N1B4_222 0.45 -1.2 282,739 + Pf1N1B4_222 0.45 +1.4 282,739 + Pf1N1B4_222 0.45 +1.3 283,224 + Pf1N1B4_222 0.75 +0.8 283,225 - Pf1N1B4_222 0.76 -2.5 283,252 + Pf1N1B4_222 0.77 -0.6 283,480 + -0.9 283,481 - -0.2 283,481 - -1.8 283,481 - -1.8 283,481 - -0.2 283,546 + +1.2 283,547 - +0.3 283,633 - +0.8 283,696 + -2.2 283,948 - Pf1N1B4_223 0.17 -0.2 283,948 - Pf1N1B4_223 0.17 -1.4 283,948 - Pf1N1B4_223 0.17 -1.0 283,954 - Pf1N1B4_223 0.17 -0.6 284,156 - Pf1N1B4_223 0.35 -2.8 284,196 + Pf1N1B4_223 0.38 -1.8 284,196 + Pf1N1B4_223 0.38 -0.5 284,197 - -0.2 284,197 - Pf1N1B4_223 0.38 -0.2 284,197 - Pf1N1B4_223 0.38 -1.8 284,332 + Pf1N1B4_223 0.50 +3.1 284,333 - Pf1N1B4_223 0.50 +1.0 284,520 + Pf1N1B4_223 0.66 -0.6 284,520 + Pf1N1B4_223 0.66 -2.5 284,520 + Pf1N1B4_223 0.66 -0.2 284,520 + Pf1N1B4_223 0.66 -0.2 284,521 - Pf1N1B4_223 0.66 +0.1 284,544 + Pf1N1B4_223 0.68 -1.6
Or see this region's nucleotide sequence