Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1832 and Pf1N1B4_1833 overlap by 4 nucleotides Pf1N1B4_1833 and Pf1N1B4_1834 are separated by 154 nucleotides Pf1N1B4_1834 and Pf1N1B4_1835 are separated by 130 nucleotides Pf1N1B4_1835 and Pf1N1B4_1836 are separated by 64 nucleotides
Pf1N1B4_1832 - Glycine cleavage system transcriptional activator, at 2,108,653 to 2,109,546
_1832
Pf1N1B4_1833 - FIG00953718: hypothetical protein, at 2,109,543 to 2,109,869
_1833
Pf1N1B4_1834 - Dodecin (COG3360) Flavin-binding, at 2,110,024 to 2,110,239
_1834
Pf1N1B4_1835 - hypothetical protein, at 2,110,370 to 2,110,594
_1835
Pf1N1B4_1836 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 2,110,659 to 2,111,660
_1836
Position (kb)
2110
2111 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2109.089 kb on + strand, within Pf1N1B4_1832 at 2109.089 kb on + strand, within Pf1N1B4_1832 at 2109.090 kb on - strand, within Pf1N1B4_1832 at 2109.090 kb on - strand, within Pf1N1B4_1832 at 2109.127 kb on + strand, within Pf1N1B4_1832 at 2109.127 kb on + strand, within Pf1N1B4_1832 at 2109.128 kb on - strand, within Pf1N1B4_1832 at 2109.307 kb on + strand, within Pf1N1B4_1832 at 2109.307 kb on + strand, within Pf1N1B4_1832 at 2109.307 kb on + strand, within Pf1N1B4_1832 at 2109.307 kb on + strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.308 kb on - strand, within Pf1N1B4_1832 at 2109.332 kb on - strand, within Pf1N1B4_1832 at 2109.332 kb on - strand at 2109.332 kb on - strand, within Pf1N1B4_1832 at 2109.332 kb on - strand, within Pf1N1B4_1832 at 2109.334 kb on + strand, within Pf1N1B4_1832 at 2109.334 kb on + strand, within Pf1N1B4_1832 at 2109.334 kb on + strand, within Pf1N1B4_1832 at 2109.335 kb on - strand, within Pf1N1B4_1832 at 2109.519 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.544 kb on + strand at 2109.545 kb on - strand at 2109.545 kb on - strand at 2109.545 kb on - strand at 2109.545 kb on - strand at 2109.545 kb on - strand at 2109.545 kb on - strand at 2109.548 kb on + strand at 2109.548 kb on + strand at 2109.548 kb on + strand at 2109.568 kb on + strand at 2109.569 kb on - strand at 2109.634 kb on + strand, within Pf1N1B4_1833 at 2109.634 kb on + strand, within Pf1N1B4_1833 at 2109.635 kb on - strand, within Pf1N1B4_1833 at 2109.635 kb on - strand, within Pf1N1B4_1833 at 2109.635 kb on - strand, within Pf1N1B4_1833 at 2109.686 kb on - strand, within Pf1N1B4_1833 at 2109.686 kb on - strand, within Pf1N1B4_1833 at 2109.759 kb on - strand, within Pf1N1B4_1833 at 2109.848 kb on + strand at 2109.848 kb on + strand at 2109.848 kb on + strand at 2109.848 kb on + strand at 2109.849 kb on - strand at 2109.849 kb on - strand at 2109.849 kb on - strand at 2109.852 kb on + strand at 2109.853 kb on - strand at 2109.853 kb on - strand at 2109.937 kb on + strand at 2109.937 kb on + strand at 2109.937 kb on + strand at 2109.938 kb on - strand at 2109.938 kb on - strand at 2109.976 kb on + strand at 2109.977 kb on - strand at 2109.977 kb on - strand at 2109.992 kb on + strand at 2109.992 kb on + strand at 2109.992 kb on + strand at 2109.992 kb on + strand at 2109.993 kb on - strand at 2109.993 kb on - strand at 2109.993 kb on - strand at 2109.993 kb on - strand at 2109.999 kb on + strand at 2109.999 kb on + strand at 2110.000 kb on - strand at 2110.000 kb on - strand at 2110.000 kb on - strand at 2110.012 kb on + strand at 2110.012 kb on + strand at 2110.042 kb on + strand at 2110.042 kb on + strand at 2110.042 kb on + strand at 2110.042 kb on + strand at 2110.042 kb on + strand at 2110.043 kb on - strand at 2110.043 kb on - strand at 2110.043 kb on - strand at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.132 kb on + strand, within Pf1N1B4_1834 at 2110.133 kb on - strand, within Pf1N1B4_1834 at 2110.133 kb on - strand, within Pf1N1B4_1834 at 2110.133 kb on - strand, within Pf1N1B4_1834 at 2110.133 kb on - strand, within Pf1N1B4_1834 at 2110.233 kb on + strand at 2110.234 kb on - strand at 2110.234 kb on - strand at 2110.234 kb on - strand at 2110.244 kb on + strand at 2110.245 kb on - strand at 2110.248 kb on + strand at 2110.248 kb on + strand at 2110.248 kb on + strand at 2110.248 kb on + strand at 2110.393 kb on - strand, within Pf1N1B4_1835 at 2110.496 kb on + strand, within Pf1N1B4_1835 at 2110.496 kb on + strand at 2110.497 kb on - strand, within Pf1N1B4_1835 at 2110.497 kb on - strand, within Pf1N1B4_1835 at 2110.580 kb on + strand at 2110.580 kb on + strand at 2110.580 kb on + strand at 2110.580 kb on + strand at 2110.581 kb on - strand at 2110.690 kb on + strand at 2110.690 kb on + strand at 2110.690 kb on + strand at 2110.690 kb on + strand at 2110.690 kb on + strand at 2110.691 kb on - strand at 2110.730 kb on + strand at 2110.730 kb on + strand at 2110.730 kb on + strand at 2110.731 kb on - strand at 2110.731 kb on - strand at 2110.868 kb on + strand at 2110.868 kb on + strand, within Pf1N1B4_1836 at 2110.869 kb on - strand, within Pf1N1B4_1836 at 2110.869 kb on - strand, within Pf1N1B4_1836 at 2110.869 kb on - strand, within Pf1N1B4_1836 at 2110.869 kb on - strand, within Pf1N1B4_1836 at 2110.869 kb on - strand, within Pf1N1B4_1836 at 2111.002 kb on + strand, within Pf1N1B4_1836 at 2111.002 kb on + strand, within Pf1N1B4_1836 at 2111.002 kb on + strand, within Pf1N1B4_1836 at 2111.003 kb on - strand, within Pf1N1B4_1836 at 2111.003 kb on - strand, within Pf1N1B4_1836 at 2111.003 kb on - strand, within Pf1N1B4_1836 at 2111.003 kb on - strand, within Pf1N1B4_1836 at 2111.003 kb on - strand, within Pf1N1B4_1836 at 2111.224 kb on + strand, within Pf1N1B4_1836
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 2,109,089 + Pf1N1B4_1832 0.49 -0.6 2,109,089 + Pf1N1B4_1832 0.49 -0.7 2,109,090 - Pf1N1B4_1832 0.49 -1.2 2,109,090 - Pf1N1B4_1832 0.49 +0.6 2,109,127 + Pf1N1B4_1832 0.53 +0.9 2,109,127 + Pf1N1B4_1832 0.53 -0.4 2,109,128 - Pf1N1B4_1832 0.53 +0.2 2,109,307 + Pf1N1B4_1832 0.73 +0.6 2,109,307 + Pf1N1B4_1832 0.73 +0.6 2,109,307 + Pf1N1B4_1832 0.73 +1.6 2,109,307 + Pf1N1B4_1832 0.73 -0.9 2,109,308 - Pf1N1B4_1832 0.73 +0.1 2,109,308 - Pf1N1B4_1832 0.73 -0.8 2,109,308 - Pf1N1B4_1832 0.73 +0.6 2,109,308 - Pf1N1B4_1832 0.73 -1.4 2,109,308 - Pf1N1B4_1832 0.73 +1.4 2,109,308 - Pf1N1B4_1832 0.73 -2.4 2,109,308 - Pf1N1B4_1832 0.73 +0.6 2,109,308 - Pf1N1B4_1832 0.73 -1.7 2,109,308 - Pf1N1B4_1832 0.73 -0.9 2,109,332 - Pf1N1B4_1832 0.76 -1.4 2,109,332 - -1.4 2,109,332 - Pf1N1B4_1832 0.76 -1.9 2,109,332 - Pf1N1B4_1832 0.76 -0.4 2,109,334 + Pf1N1B4_1832 0.76 -0.9 2,109,334 + Pf1N1B4_1832 0.76 +1.6 2,109,334 + Pf1N1B4_1832 0.76 -0.4 2,109,335 - Pf1N1B4_1832 0.76 -2.4 2,109,519 + +0.1 2,109,544 + -0.9 2,109,544 + +1.1 2,109,544 + +1.6 2,109,544 + -1.9 2,109,544 + -0.4 2,109,544 + -1.7 2,109,544 + -0.4 2,109,544 + +2.1 2,109,544 + +0.5 2,109,544 + +0.0 2,109,544 + -0.4 2,109,544 + -0.5 2,109,545 - +0.6 2,109,545 - -0.9 2,109,545 - +1.1 2,109,545 - -0.9 2,109,545 - -0.9 2,109,545 - -1.1 2,109,548 + +0.3 2,109,548 + -0.1 2,109,548 + +0.2 2,109,568 + +0.1 2,109,569 - -0.4 2,109,634 + Pf1N1B4_1833 0.28 +0.6 2,109,634 + Pf1N1B4_1833 0.28 +0.6 2,109,635 - Pf1N1B4_1833 0.28 +1.4 2,109,635 - Pf1N1B4_1833 0.28 -2.8 2,109,635 - Pf1N1B4_1833 0.28 -0.1 2,109,686 - Pf1N1B4_1833 0.44 +0.3 2,109,686 - Pf1N1B4_1833 0.44 -0.8 2,109,759 - Pf1N1B4_1833 0.66 +0.6 2,109,848 + -1.4 2,109,848 + -0.8 2,109,848 + -1.7 2,109,848 + -0.4 2,109,849 - -0.9 2,109,849 - -1.7 2,109,849 - +1.4 2,109,852 + -2.4 2,109,853 - -2.4 2,109,853 - -0.9 2,109,937 + -1.4 2,109,937 + -1.2 2,109,937 + -0.4 2,109,938 - -0.1 2,109,938 - +0.5 2,109,976 + +2.8 2,109,977 - -1.4 2,109,977 - +0.1 2,109,992 + +0.6 2,109,992 + +2.1 2,109,992 + -0.4 2,109,992 + +0.6 2,109,993 - -1.4 2,109,993 - -1.7 2,109,993 - -0.0 2,109,993 - +1.0 2,109,999 + -1.9 2,109,999 + +2.6 2,110,000 - -0.8 2,110,000 - +2.0 2,110,000 - -0.0 2,110,012 + +0.6 2,110,012 + -1.9 2,110,042 + +1.5 2,110,042 + +0.1 2,110,042 + +1.6 2,110,042 + -2.2 2,110,042 + +1.2 2,110,043 - +1.6 2,110,043 - -1.4 2,110,043 - -0.9 2,110,132 + Pf1N1B4_1834 0.50 -0.7 2,110,132 + Pf1N1B4_1834 0.50 +0.9 2,110,132 + Pf1N1B4_1834 0.50 +1.5 2,110,132 + Pf1N1B4_1834 0.50 -0.4 2,110,132 + Pf1N1B4_1834 0.50 -1.2 2,110,132 + Pf1N1B4_1834 0.50 -0.4 2,110,132 + Pf1N1B4_1834 0.50 -0.0 2,110,132 + Pf1N1B4_1834 0.50 +1.6 2,110,132 + Pf1N1B4_1834 0.50 -0.7 2,110,132 + Pf1N1B4_1834 0.50 +0.9 2,110,132 + Pf1N1B4_1834 0.50 +1.1 2,110,132 + Pf1N1B4_1834 0.50 +0.3 2,110,133 - Pf1N1B4_1834 0.50 +0.6 2,110,133 - Pf1N1B4_1834 0.50 +1.5 2,110,133 - Pf1N1B4_1834 0.50 +1.2 2,110,133 - Pf1N1B4_1834 0.50 -1.7 2,110,233 + -1.4 2,110,234 - +0.6 2,110,234 - -0.8 2,110,234 - -1.4 2,110,244 + +0.6 2,110,245 - +0.6 2,110,248 + +0.6 2,110,248 + +3.2 2,110,248 + -0.4 2,110,248 + +0.8 2,110,393 - Pf1N1B4_1835 0.10 -0.7 2,110,496 + Pf1N1B4_1835 0.56 +0.4 2,110,496 + +0.6 2,110,497 - Pf1N1B4_1835 0.56 -0.9 2,110,497 - Pf1N1B4_1835 0.56 +1.1 2,110,580 + -0.9 2,110,580 + -0.9 2,110,580 + +0.4 2,110,580 + -0.1 2,110,581 - +1.2 2,110,690 + -0.5 2,110,690 + +0.6 2,110,690 + +0.2 2,110,690 + +1.5 2,110,690 + +0.9 2,110,691 - -1.4 2,110,730 + -0.4 2,110,730 + +0.6 2,110,730 + -0.9 2,110,731 - +2.5 2,110,731 - +0.3 2,110,868 + -0.9 2,110,868 + Pf1N1B4_1836 0.21 -1.4 2,110,869 - Pf1N1B4_1836 0.21 -0.4 2,110,869 - Pf1N1B4_1836 0.21 -0.4 2,110,869 - Pf1N1B4_1836 0.21 -1.4 2,110,869 - Pf1N1B4_1836 0.21 +1.1 2,110,869 - Pf1N1B4_1836 0.21 -0.9 2,111,002 + Pf1N1B4_1836 0.34 -0.5 2,111,002 + Pf1N1B4_1836 0.34 -1.7 2,111,002 + Pf1N1B4_1836 0.34 -0.8 2,111,003 - Pf1N1B4_1836 0.34 +2.6 2,111,003 - Pf1N1B4_1836 0.34 +0.4 2,111,003 - Pf1N1B4_1836 0.34 -0.9 2,111,003 - Pf1N1B4_1836 0.34 -0.1 2,111,003 - Pf1N1B4_1836 0.34 +1.6 2,111,224 + Pf1N1B4_1836 0.56 +1.9
Or see this region's nucleotide sequence