Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_180 and Pf1N1B4_181 are separated by 127 nucleotides Pf1N1B4_181 and Pf1N1B4_182 overlap by 4 nucleotides Pf1N1B4_182 and Pf1N1B4_183 overlap by 4 nucleotides
Pf1N1B4_180 - L-Proline/Glycine betaine transporter ProP, at 222,785 to 224,164
_180
Pf1N1B4_181 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 224,292 to 225,233
_181
Pf1N1B4_182 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 225,230 to 225,985
_182
Pf1N1B4_183 - Major facilitator family transporter, at 225,982 to 227,283
_183
Position (kb) 224
225
226 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 223.344 kb on + strand, within Pf1N1B4_180 at 223.524 kb on + strand, within Pf1N1B4_180 at 223.524 kb on + strand, within Pf1N1B4_180 at 223.525 kb on - strand, within Pf1N1B4_180 at 223.668 kb on - strand, within Pf1N1B4_180 at 223.680 kb on + strand, within Pf1N1B4_180 at 223.815 kb on + strand, within Pf1N1B4_180 at 223.816 kb on - strand, within Pf1N1B4_180 at 223.945 kb on - strand, within Pf1N1B4_180 at 223.973 kb on + strand, within Pf1N1B4_180 at 223.979 kb on - strand, within Pf1N1B4_180 at 224.145 kb on - strand at 224.165 kb on + strand at 224.196 kb on - strand at 224.196 kb on - strand at 224.196 kb on - strand at 224.200 kb on - strand at 224.249 kb on - strand at 224.294 kb on - strand at 224.364 kb on + strand at 224.707 kb on - strand, within Pf1N1B4_181 at 224.926 kb on + strand, within Pf1N1B4_181 at 224.927 kb on - strand, within Pf1N1B4_181 at 225.240 kb on + strand at 225.240 kb on + strand at 225.240 kb on + strand at 225.240 kb on + strand at 225.241 kb on - strand at 225.241 kb on - strand at 225.334 kb on + strand at 225.335 kb on - strand, within Pf1N1B4_182 at 225.558 kb on + strand at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.558 kb on + strand, within Pf1N1B4_182 at 225.559 kb on - strand, within Pf1N1B4_182 at 225.559 kb on - strand, within Pf1N1B4_182 at 225.559 kb on - strand at 225.559 kb on - strand, within Pf1N1B4_182 at 225.559 kb on - strand at 225.748 kb on - strand, within Pf1N1B4_182 at 225.748 kb on - strand, within Pf1N1B4_182 at 225.875 kb on - strand, within Pf1N1B4_182 at 225.980 kb on - strand at 225.980 kb on - strand at 225.989 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 223,344 + Pf1N1B4_180 0.41 -0.6 223,524 + Pf1N1B4_180 0.54 -1.2 223,524 + Pf1N1B4_180 0.54 -1.2 223,525 - Pf1N1B4_180 0.54 +1.8 223,668 - Pf1N1B4_180 0.64 -0.2 223,680 + Pf1N1B4_180 0.65 +0.1 223,815 + Pf1N1B4_180 0.75 -3.2 223,816 - Pf1N1B4_180 0.75 -3.5 223,945 - Pf1N1B4_180 0.84 +0.0 223,973 + Pf1N1B4_180 0.86 +1.4 223,979 - Pf1N1B4_180 0.87 -1.8 224,145 - -0.8 224,165 + -1.2 224,196 - +0.5 224,196 - -0.2 224,196 - -0.0 224,200 - +0.5 224,249 - -0.2 224,294 - -2.2 224,364 + +1.0 224,707 - Pf1N1B4_181 0.44 -1.8 224,926 + Pf1N1B4_181 0.67 -1.2 224,927 - Pf1N1B4_181 0.67 -0.5 225,240 + +0.8 225,240 + -1.8 225,240 + -0.6 225,240 + -0.4 225,241 - +0.5 225,241 - -0.2 225,334 + +1.1 225,335 - Pf1N1B4_182 0.14 +0.1 225,558 + +0.4 225,558 + Pf1N1B4_182 0.43 +0.6 225,558 + Pf1N1B4_182 0.43 -1.2 225,558 + Pf1N1B4_182 0.43 -2.2 225,558 + Pf1N1B4_182 0.43 +1.4 225,558 + Pf1N1B4_182 0.43 -0.9 225,559 - Pf1N1B4_182 0.44 -0.6 225,559 - Pf1N1B4_182 0.44 -0.8 225,559 - -0.2 225,559 - Pf1N1B4_182 0.44 +2.5 225,559 - +1.8 225,748 - Pf1N1B4_182 0.69 +1.4 225,748 - Pf1N1B4_182 0.69 -0.2 225,875 - Pf1N1B4_182 0.85 -2.2 225,980 - +1.6 225,980 - -0.5 225,989 + +0.2
Or see this region's nucleotide sequence