Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1327 and Pf1N1B4_1328 overlap by 4 nucleotides Pf1N1B4_1328 and Pf1N1B4_1329 overlap by 8 nucleotides Pf1N1B4_1329 and Pf1N1B4_1330 are separated by 145 nucleotides
Pf1N1B4_1327 - Cobalt-precorrin-2 C20-methyltransferase (EC 2.1.1.130), at 1,522,428 to 1,523,162
_1327
Pf1N1B4_1328 - Cobalt-precorrin-8x methylmutase (EC 5.4.1.2), at 1,523,159 to 1,523,785
_1328
Pf1N1B4_1329 - Cobalamin biosynthesis protein CobG, at 1,523,778 to 1,525,079
_1329
Pf1N1B4_1330 - hypothetical protein, at 1,525,225 to 1,525,881
_1330
Position (kb)
1523
1524
1525
1526 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1522.778 kb on - strand, within Pf1N1B4_1327 at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.804 kb on + strand at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.804 kb on + strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.805 kb on - strand at 1522.805 kb on - strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.810 kb on + strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.811 kb on - strand, within Pf1N1B4_1327 at 1522.819 kb on + strand, within Pf1N1B4_1327 at 1522.820 kb on - strand, within Pf1N1B4_1327 at 1522.903 kb on + strand, within Pf1N1B4_1327 at 1522.904 kb on - strand, within Pf1N1B4_1327 at 1522.904 kb on - strand, within Pf1N1B4_1327 at 1522.904 kb on - strand, within Pf1N1B4_1327 at 1522.904 kb on - strand, within Pf1N1B4_1327 at 1522.928 kb on - strand, within Pf1N1B4_1327 at 1523.054 kb on - strand, within Pf1N1B4_1327 at 1523.054 kb on - strand, within Pf1N1B4_1327 at 1523.054 kb on - strand, within Pf1N1B4_1327 at 1523.054 kb on - strand, within Pf1N1B4_1327 at 1523.361 kb on + strand at 1523.361 kb on + strand, within Pf1N1B4_1328 at 1523.361 kb on + strand, within Pf1N1B4_1328 at 1523.361 kb on + strand, within Pf1N1B4_1328 at 1523.362 kb on - strand, within Pf1N1B4_1328 at 1523.362 kb on - strand, within Pf1N1B4_1328 at 1523.362 kb on - strand, within Pf1N1B4_1328 at 1523.362 kb on - strand, within Pf1N1B4_1328 at 1523.751 kb on + strand at 1523.751 kb on + strand at 1523.751 kb on + strand at 1523.751 kb on + strand at 1523.752 kb on - strand at 1523.752 kb on - strand at 1523.775 kb on + strand at 1523.775 kb on + strand at 1523.775 kb on + strand at 1523.775 kb on + strand at 1523.776 kb on - strand at 1523.776 kb on - strand at 1523.776 kb on - strand at 1523.776 kb on - strand at 1523.776 kb on - strand at 1523.776 kb on - strand at 1523.776 kb on - strand at 1523.834 kb on + strand at 1523.834 kb on + strand at 1523.834 kb on + strand at 1523.835 kb on - strand at 1523.835 kb on - strand at 1523.835 kb on - strand at 1523.835 kb on - strand at 1523.835 kb on - strand at 1523.837 kb on + strand at 1523.837 kb on + strand at 1523.838 kb on - strand at 1523.838 kb on - strand at 1523.838 kb on - strand at 1523.838 kb on - strand at 1523.838 kb on - strand at 1523.890 kb on + strand at 1523.891 kb on - strand at 1524.283 kb on - strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.301 kb on + strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.758 kb on + strand, within Pf1N1B4_1329 at 1524.758 kb on + strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1525.065 kb on + strand at 1525.066 kb on - strand at 1525.145 kb on - strand at 1525.201 kb on - strand at 1525.201 kb on - strand at 1525.208 kb on - strand at 1525.236 kb on - strand at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.383 kb on + strand, within Pf1N1B4_1330 at 1525.384 kb on - strand, within Pf1N1B4_1330 at 1525.448 kb on + strand, within Pf1N1B4_1330 at 1525.464 kb on + strand, within Pf1N1B4_1330 at 1525.729 kb on - strand, within Pf1N1B4_1330 at 1525.731 kb on - strand, within Pf1N1B4_1330 at 1525.731 kb on - strand, within Pf1N1B4_1330 at 1525.759 kb on + strand, within Pf1N1B4_1330 at 1525.825 kb on - strand at 1525.825 kb on - strand at 1525.825 kb on - strand at 1525.882 kb on - strand at 1525.975 kb on + strand at 1526.016 kb on + strand at 1526.016 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 1,522,778 - Pf1N1B4_1327 0.48 +1.9 1,522,804 + Pf1N1B4_1327 0.51 -0.9 1,522,804 + Pf1N1B4_1327 0.51 -0.0 1,522,804 + +1.4 1,522,804 + Pf1N1B4_1327 0.51 -1.2 1,522,804 + Pf1N1B4_1327 0.51 +0.1 1,522,804 + Pf1N1B4_1327 0.51 +0.8 1,522,805 - Pf1N1B4_1327 0.51 +0.4 1,522,805 - Pf1N1B4_1327 0.51 -1.2 1,522,805 - Pf1N1B4_1327 0.51 -0.6 1,522,805 - Pf1N1B4_1327 0.51 -0.6 1,522,805 - Pf1N1B4_1327 0.51 -1.6 1,522,805 - Pf1N1B4_1327 0.51 -1.2 1,522,805 - Pf1N1B4_1327 0.51 +0.3 1,522,805 - -0.6 1,522,805 - Pf1N1B4_1327 0.51 -1.2 1,522,810 + Pf1N1B4_1327 0.52 -1.9 1,522,810 + Pf1N1B4_1327 0.52 +0.6 1,522,810 + Pf1N1B4_1327 0.52 -0.6 1,522,810 + Pf1N1B4_1327 0.52 -1.6 1,522,810 + Pf1N1B4_1327 0.52 -0.3 1,522,810 + Pf1N1B4_1327 0.52 +1.4 1,522,810 + Pf1N1B4_1327 0.52 +0.2 1,522,810 + Pf1N1B4_1327 0.52 -1.2 1,522,810 + Pf1N1B4_1327 0.52 -0.7 1,522,810 + Pf1N1B4_1327 0.52 -2.2 1,522,810 + Pf1N1B4_1327 0.52 -1.6 1,522,810 + Pf1N1B4_1327 0.52 -2.4 1,522,811 - Pf1N1B4_1327 0.52 -0.4 1,522,811 - Pf1N1B4_1327 0.52 +2.3 1,522,811 - Pf1N1B4_1327 0.52 -1.2 1,522,811 - Pf1N1B4_1327 0.52 +2.4 1,522,811 - Pf1N1B4_1327 0.52 +0.1 1,522,811 - Pf1N1B4_1327 0.52 -1.3 1,522,811 - Pf1N1B4_1327 0.52 -2.6 1,522,811 - Pf1N1B4_1327 0.52 -0.3 1,522,811 - Pf1N1B4_1327 0.52 -1.8 1,522,811 - Pf1N1B4_1327 0.52 -1.9 1,522,811 - Pf1N1B4_1327 0.52 -0.2 1,522,811 - Pf1N1B4_1327 0.52 -1.9 1,522,811 - Pf1N1B4_1327 0.52 -1.6 1,522,819 + Pf1N1B4_1327 0.53 -0.2 1,522,820 - Pf1N1B4_1327 0.53 -0.6 1,522,903 + Pf1N1B4_1327 0.65 +0.4 1,522,904 - Pf1N1B4_1327 0.65 -0.2 1,522,904 - Pf1N1B4_1327 0.65 +0.0 1,522,904 - Pf1N1B4_1327 0.65 -0.4 1,522,904 - Pf1N1B4_1327 0.65 -1.3 1,522,928 - Pf1N1B4_1327 0.68 +0.4 1,523,054 - Pf1N1B4_1327 0.85 -0.0 1,523,054 - Pf1N1B4_1327 0.85 -1.2 1,523,054 - Pf1N1B4_1327 0.85 -0.2 1,523,054 - Pf1N1B4_1327 0.85 +1.1 1,523,361 + -1.2 1,523,361 + Pf1N1B4_1328 0.32 +0.2 1,523,361 + Pf1N1B4_1328 0.32 -1.6 1,523,361 + Pf1N1B4_1328 0.32 -2.2 1,523,362 - Pf1N1B4_1328 0.32 -0.1 1,523,362 - Pf1N1B4_1328 0.32 +1.4 1,523,362 - Pf1N1B4_1328 0.32 -0.8 1,523,362 - Pf1N1B4_1328 0.32 +1.6 1,523,751 + -1.2 1,523,751 + +0.1 1,523,751 + +0.9 1,523,751 + -0.3 1,523,752 - -0.6 1,523,752 - -0.6 1,523,775 + -2.4 1,523,775 + -0.6 1,523,775 + -1.4 1,523,775 + -1.6 1,523,776 - -0.0 1,523,776 - -0.9 1,523,776 - -2.6 1,523,776 - -1.6 1,523,776 - +0.1 1,523,776 - +2.6 1,523,776 - -0.5 1,523,834 + -1.9 1,523,834 + +1.4 1,523,834 + +1.1 1,523,835 - -1.6 1,523,835 - -0.8 1,523,835 - -0.2 1,523,835 - +0.1 1,523,835 - -2.8 1,523,837 + -1.4 1,523,837 + -0.6 1,523,838 - -1.5 1,523,838 - -1.2 1,523,838 - -0.0 1,523,838 - -1.6 1,523,838 - -0.0 1,523,890 + -0.0 1,523,891 - -1.9 1,524,283 - Pf1N1B4_1329 0.39 +2.0 1,524,295 + Pf1N1B4_1329 0.40 -0.6 1,524,295 + Pf1N1B4_1329 0.40 -1.2 1,524,295 + +0.4 1,524,295 + Pf1N1B4_1329 0.40 +0.7 1,524,295 + Pf1N1B4_1329 0.40 -0.6 1,524,295 + Pf1N1B4_1329 0.40 -1.8 1,524,295 + Pf1N1B4_1329 0.40 -0.6 1,524,296 - Pf1N1B4_1329 0.40 -2.9 1,524,296 - Pf1N1B4_1329 0.40 +1.4 1,524,296 - Pf1N1B4_1329 0.40 -0.0 1,524,296 - Pf1N1B4_1329 0.40 -2.8 1,524,296 - Pf1N1B4_1329 0.40 -1.2 1,524,296 - Pf1N1B4_1329 0.40 -2.8 1,524,296 - Pf1N1B4_1329 0.40 -1.6 1,524,296 - Pf1N1B4_1329 0.40 -0.8 1,524,296 - Pf1N1B4_1329 0.40 -1.0 1,524,296 - Pf1N1B4_1329 0.40 -1.9 1,524,296 - Pf1N1B4_1329 0.40 -2.2 1,524,296 - Pf1N1B4_1329 0.40 -1.2 1,524,296 - Pf1N1B4_1329 0.40 -1.1 1,524,301 + Pf1N1B4_1329 0.40 -2.6 1,524,302 - Pf1N1B4_1329 0.40 -0.4 1,524,302 - Pf1N1B4_1329 0.40 +0.1 1,524,302 - Pf1N1B4_1329 0.40 -0.2 1,524,302 - Pf1N1B4_1329 0.40 -0.0 1,524,302 - Pf1N1B4_1329 0.40 -1.2 1,524,758 + Pf1N1B4_1329 0.75 -0.9 1,524,758 + Pf1N1B4_1329 0.75 -2.1 1,524,759 - Pf1N1B4_1329 0.75 -1.6 1,524,759 - Pf1N1B4_1329 0.75 -1.2 1,524,759 - Pf1N1B4_1329 0.75 -1.2 1,524,759 - Pf1N1B4_1329 0.75 -0.3 1,525,065 + -1.6 1,525,066 - -1.9 1,525,145 - -0.8 1,525,201 - -0.3 1,525,201 - -0.2 1,525,208 - -1.3 1,525,236 - -0.6 1,525,377 - Pf1N1B4_1330 0.23 +0.4 1,525,377 - Pf1N1B4_1330 0.23 -0.4 1,525,377 - Pf1N1B4_1330 0.23 -1.2 1,525,383 + Pf1N1B4_1330 0.24 +2.4 1,525,384 - Pf1N1B4_1330 0.24 +0.4 1,525,448 + Pf1N1B4_1330 0.34 -0.6 1,525,464 + Pf1N1B4_1330 0.36 -1.6 1,525,729 - Pf1N1B4_1330 0.77 +0.4 1,525,731 - Pf1N1B4_1330 0.77 +2.3 1,525,731 - Pf1N1B4_1330 0.77 +0.9 1,525,759 + Pf1N1B4_1330 0.81 +0.4 1,525,825 - +0.2 1,525,825 - +1.4 1,525,825 - +0.4 1,525,882 - -1.6 1,525,975 + -1.2 1,526,016 + -1.9 1,526,016 + +0.7
Or see this region's nucleotide sequence