Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1086 and Pf1N1B4_1087 are separated by 1 nucleotides Pf1N1B4_1087 and Pf1N1B4_1088 are separated by 27 nucleotides Pf1N1B4_1088 and Pf1N1B4_1089 overlap by 8 nucleotides Pf1N1B4_1089 and Pf1N1B4_1090 are separated by 247 nucleotides
Pf1N1B4_1086 - Manganese superoxide dismutase (EC 1.15.1.1), at 1,216,684 to 1,217,295
_1086
Pf1N1B4_1087 - FOG: TPR repeat, at 1,217,297 to 1,217,737
_1087
Pf1N1B4_1088 - Fumarate hydratase class II (EC 4.2.1.2), at 1,217,765 to 1,219,141
_1088
Pf1N1B4_1089 - FagA protein, at 1,219,134 to 1,219,529
_1089
Pf1N1B4_1090 - TldE protein, part of TldE/TldD proteolytic complex, at 1,219,777 to 1,221,123
_1090
Position (kb)
1217
1218
1219
1220 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1216.784 kb on + strand, within Pf1N1B4_1086 at 1216.784 kb on + strand, within Pf1N1B4_1086 at 1216.785 kb on - strand, within Pf1N1B4_1086 at 1216.785 kb on - strand, within Pf1N1B4_1086 at 1216.785 kb on - strand, within Pf1N1B4_1086 at 1216.788 kb on - strand, within Pf1N1B4_1086 at 1216.824 kb on + strand, within Pf1N1B4_1086 at 1217.192 kb on + strand, within Pf1N1B4_1086 at 1217.192 kb on + strand, within Pf1N1B4_1086 at 1217.193 kb on - strand, within Pf1N1B4_1086 at 1217.193 kb on - strand, within Pf1N1B4_1086 at 1217.193 kb on - strand, within Pf1N1B4_1086 at 1217.213 kb on + strand, within Pf1N1B4_1086 at 1217.261 kb on + strand at 1217.261 kb on + strand at 1217.286 kb on + strand at 1217.286 kb on + strand at 1217.286 kb on + strand at 1217.287 kb on - strand at 1217.287 kb on - strand at 1217.315 kb on + strand at 1217.315 kb on + strand at 1217.316 kb on - strand at 1217.316 kb on - strand at 1217.316 kb on - strand at 1217.418 kb on + strand, within Pf1N1B4_1087 at 1217.418 kb on + strand at 1217.418 kb on + strand at 1217.419 kb on - strand, within Pf1N1B4_1087 at 1217.489 kb on + strand, within Pf1N1B4_1087 at 1217.489 kb on + strand, within Pf1N1B4_1087 at 1217.570 kb on - strand, within Pf1N1B4_1087 at 1217.658 kb on + strand, within Pf1N1B4_1087 at 1217.658 kb on + strand, within Pf1N1B4_1087 at 1217.659 kb on - strand, within Pf1N1B4_1087 at 1217.659 kb on - strand, within Pf1N1B4_1087 at 1217.766 kb on + strand at 1217.767 kb on - strand at 1217.874 kb on + strand at 1217.874 kb on + strand at 1219.100 kb on + strand at 1219.100 kb on + strand at 1219.100 kb on + strand at 1219.100 kb on + strand at 1219.101 kb on - strand at 1219.101 kb on - strand at 1219.101 kb on - strand at 1219.101 kb on - strand at 1219.101 kb on - strand at 1219.101 kb on - strand at 1219.101 kb on - strand at 1219.135 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.164 kb on + strand at 1219.213 kb on + strand at 1219.501 kb on + strand at 1219.501 kb on + strand at 1219.502 kb on - strand at 1219.640 kb on + strand at 1219.641 kb on - strand at 1219.721 kb on - strand at 1219.778 kb on + strand at 1219.778 kb on + strand at 1219.778 kb on + strand at 1219.778 kb on + strand at 1219.779 kb on - strand at 1219.779 kb on - strand at 1219.779 kb on - strand at 1219.779 kb on - strand at 1219.779 kb on - strand at 1219.783 kb on - strand at 1219.828 kb on - strand at 1219.828 kb on - strand at 1219.851 kb on + strand at 1219.899 kb on + strand at 1219.967 kb on + strand, within Pf1N1B4_1090 at 1220.135 kb on + strand, within Pf1N1B4_1090 at 1220.136 kb on - strand, within Pf1N1B4_1090 at 1220.136 kb on - strand, within Pf1N1B4_1090
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 1,216,784 + Pf1N1B4_1086 0.16 -1.2 1,216,784 + Pf1N1B4_1086 0.16 -0.1 1,216,785 - Pf1N1B4_1086 0.17 -0.4 1,216,785 - Pf1N1B4_1086 0.17 -1.2 1,216,785 - Pf1N1B4_1086 0.17 +2.3 1,216,788 - Pf1N1B4_1086 0.17 -1.2 1,216,824 + Pf1N1B4_1086 0.23 -0.9 1,217,192 + Pf1N1B4_1086 0.83 -1.2 1,217,192 + Pf1N1B4_1086 0.83 +0.3 1,217,193 - Pf1N1B4_1086 0.83 -1.5 1,217,193 - Pf1N1B4_1086 0.83 +0.0 1,217,193 - Pf1N1B4_1086 0.83 -1.8 1,217,213 + Pf1N1B4_1086 0.86 +2.7 1,217,261 + +0.0 1,217,261 + -0.7 1,217,286 + -0.8 1,217,286 + -2.8 1,217,286 + -0.7 1,217,287 - +0.9 1,217,287 - -1.7 1,217,315 + -2.0 1,217,315 + -0.7 1,217,316 - +2.9 1,217,316 - +0.9 1,217,316 - -2.0 1,217,418 + Pf1N1B4_1087 0.27 +0.8 1,217,418 + +0.3 1,217,418 + +2.9 1,217,419 - Pf1N1B4_1087 0.28 +0.1 1,217,489 + Pf1N1B4_1087 0.44 +2.5 1,217,489 + Pf1N1B4_1087 0.44 -1.2 1,217,570 - Pf1N1B4_1087 0.62 -0.0 1,217,658 + Pf1N1B4_1087 0.82 -1.3 1,217,658 + Pf1N1B4_1087 0.82 -2.0 1,217,659 - Pf1N1B4_1087 0.82 -1.2 1,217,659 - Pf1N1B4_1087 0.82 -1.7 1,217,766 + +0.3 1,217,767 - -0.7 1,217,874 + +1.0 1,217,874 + +1.6 1,219,100 + -0.0 1,219,100 + +0.0 1,219,100 + -0.2 1,219,100 + -1.2 1,219,101 - -1.2 1,219,101 - +0.8 1,219,101 - -2.8 1,219,101 - +0.3 1,219,101 - -0.1 1,219,101 - +1.3 1,219,101 - -1.2 1,219,135 + +0.3 1,219,164 + -2.0 1,219,164 + -1.2 1,219,164 + -1.5 1,219,164 + -0.7 1,219,164 + +0.6 1,219,164 + -0.1 1,219,164 + -2.1 1,219,164 + -0.7 1,219,213 + +1.9 1,219,501 + -1.2 1,219,501 + +0.9 1,219,502 - +1.1 1,219,640 + -2.2 1,219,641 - +1.8 1,219,721 - -2.2 1,219,778 + -0.7 1,219,778 + +0.3 1,219,778 + +0.3 1,219,778 + -0.7 1,219,779 - +1.6 1,219,779 - -1.2 1,219,779 - +0.6 1,219,779 - -1.7 1,219,779 - -0.1 1,219,783 - -1.7 1,219,828 - +1.5 1,219,828 - -0.7 1,219,851 + -1.7 1,219,899 + +1.1 1,219,967 + Pf1N1B4_1090 0.14 -2.2 1,220,135 + Pf1N1B4_1090 0.27 -1.2 1,220,136 - Pf1N1B4_1090 0.27 -0.2 1,220,136 - Pf1N1B4_1090 0.27 +0.3
Or see this region's nucleotide sequence