Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1038 and Pf1N1B4_1039 are separated by 206 nucleotides Pf1N1B4_1039 and Pf1N1B4_1040 are separated by 89 nucleotides Pf1N1B4_1040 and Pf1N1B4_1041 are separated by 22 nucleotides
Pf1N1B4_1038 - Transcriptional regulator, MerR family, at 1,176,975 to 1,177,934
_1038
Pf1N1B4_1039 - putative membrane protein, at 1,178,141 to 1,178,710
_1039
Pf1N1B4_1040 - FolM Alternative dihydrofolate reductase 1, at 1,178,800 to 1,179,510
_1040
Pf1N1B4_1041 - GTP cyclohydrolase I (EC 3.5.4.16) type 1, at 1,179,533 to 1,180,108
_1041
Position (kb)
1178
1179 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1177.185 kb on + strand, within Pf1N1B4_1038 at 1177.263 kb on - strand, within Pf1N1B4_1038 at 1177.285 kb on + strand, within Pf1N1B4_1038 at 1177.286 kb on - strand, within Pf1N1B4_1038 at 1177.286 kb on - strand, within Pf1N1B4_1038 at 1177.286 kb on - strand, within Pf1N1B4_1038 at 1177.458 kb on + strand, within Pf1N1B4_1038 at 1177.458 kb on + strand, within Pf1N1B4_1038 at 1177.458 kb on + strand, within Pf1N1B4_1038 at 1177.458 kb on + strand, within Pf1N1B4_1038 at 1177.459 kb on - strand, within Pf1N1B4_1038 at 1177.459 kb on - strand, within Pf1N1B4_1038 at 1177.459 kb on - strand, within Pf1N1B4_1038 at 1177.464 kb on + strand at 1177.464 kb on + strand, within Pf1N1B4_1038 at 1177.464 kb on + strand, within Pf1N1B4_1038 at 1177.464 kb on + strand, within Pf1N1B4_1038 at 1177.464 kb on + strand, within Pf1N1B4_1038 at 1177.465 kb on - strand, within Pf1N1B4_1038 at 1177.465 kb on - strand, within Pf1N1B4_1038 at 1177.465 kb on - strand, within Pf1N1B4_1038 at 1177.465 kb on - strand, within Pf1N1B4_1038 at 1177.465 kb on - strand, within Pf1N1B4_1038 at 1177.465 kb on - strand, within Pf1N1B4_1038 at 1177.535 kb on - strand, within Pf1N1B4_1038 at 1177.535 kb on - strand, within Pf1N1B4_1038 at 1177.603 kb on + strand, within Pf1N1B4_1038 at 1177.672 kb on - strand, within Pf1N1B4_1038 at 1177.708 kb on - strand, within Pf1N1B4_1038 at 1177.708 kb on - strand, within Pf1N1B4_1038 at 1177.753 kb on + strand, within Pf1N1B4_1038 at 1177.754 kb on - strand, within Pf1N1B4_1038 at 1177.754 kb on - strand, within Pf1N1B4_1038 at 1177.788 kb on + strand, within Pf1N1B4_1038 at 1177.789 kb on - strand, within Pf1N1B4_1038 at 1177.789 kb on - strand, within Pf1N1B4_1038 at 1177.818 kb on + strand, within Pf1N1B4_1038 at 1177.942 kb on + strand at 1177.942 kb on + strand at 1177.942 kb on + strand at 1177.942 kb on + strand at 1177.943 kb on - strand at 1177.943 kb on - strand at 1177.952 kb on + strand at 1177.952 kb on + strand at 1177.952 kb on + strand at 1177.953 kb on - strand at 1178.010 kb on + strand at 1178.011 kb on - strand at 1178.105 kb on - strand at 1178.146 kb on + strand at 1178.147 kb on - strand at 1178.272 kb on - strand, within Pf1N1B4_1039 at 1178.308 kb on + strand, within Pf1N1B4_1039 at 1178.309 kb on - strand, within Pf1N1B4_1039 at 1178.309 kb on - strand, within Pf1N1B4_1039 at 1178.532 kb on + strand, within Pf1N1B4_1039 at 1178.533 kb on - strand, within Pf1N1B4_1039 at 1178.612 kb on + strand, within Pf1N1B4_1039 at 1178.612 kb on + strand, within Pf1N1B4_1039 at 1178.613 kb on - strand, within Pf1N1B4_1039 at 1178.613 kb on - strand, within Pf1N1B4_1039 at 1178.613 kb on - strand, within Pf1N1B4_1039 at 1178.613 kb on - strand, within Pf1N1B4_1039 at 1178.613 kb on - strand, within Pf1N1B4_1039 at 1178.718 kb on + strand at 1178.719 kb on - strand at 1178.719 kb on - strand at 1178.742 kb on + strand at 1178.742 kb on + strand at 1178.742 kb on + strand at 1178.742 kb on + strand at 1178.742 kb on + strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.743 kb on - strand at 1178.767 kb on - strand at 1178.769 kb on - strand at 1178.769 kb on - strand at 1178.781 kb on - strand at 1178.808 kb on + strand at 1178.809 kb on - strand at 1179.120 kb on + strand, within Pf1N1B4_1040 at 1179.120 kb on + strand, within Pf1N1B4_1040 at 1179.120 kb on + strand, within Pf1N1B4_1040 at 1179.121 kb on - strand, within Pf1N1B4_1040 at 1179.121 kb on - strand, within Pf1N1B4_1040 at 1179.121 kb on - strand at 1179.338 kb on + strand, within Pf1N1B4_1040 at 1179.338 kb on + strand at 1179.339 kb on - strand, within Pf1N1B4_1040 at 1179.427 kb on + strand, within Pf1N1B4_1040 at 1179.427 kb on + strand, within Pf1N1B4_1040 at 1179.427 kb on + strand, within Pf1N1B4_1040 at 1179.427 kb on + strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.442 kb on + strand at 1179.460 kb on + strand at 1179.460 kb on + strand at 1179.460 kb on + strand at 1179.461 kb on - strand at 1179.461 kb on - strand at 1179.461 kb on - strand at 1179.508 kb on + strand at 1179.508 kb on + strand at 1179.508 kb on + strand at 1179.508 kb on + strand at 1179.508 kb on + strand at 1179.541 kb on - strand at 1179.573 kb on - strand at 1179.659 kb on + strand, within Pf1N1B4_1041 at 1179.659 kb on + strand at 1179.659 kb on + strand, within Pf1N1B4_1041 at 1179.660 kb on - strand, within Pf1N1B4_1041 at 1179.660 kb on - strand at 1179.660 kb on - strand, within Pf1N1B4_1041 at 1179.660 kb on - strand, within Pf1N1B4_1041 at 1179.674 kb on + strand, within Pf1N1B4_1041 at 1179.675 kb on - strand, within Pf1N1B4_1041
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 1,177,185 + Pf1N1B4_1038 0.22 -1.3 1,177,263 - Pf1N1B4_1038 0.30 -0.0 1,177,285 + Pf1N1B4_1038 0.32 -1.4 1,177,286 - Pf1N1B4_1038 0.32 +0.7 1,177,286 - Pf1N1B4_1038 0.32 -0.0 1,177,286 - Pf1N1B4_1038 0.32 +1.9 1,177,458 + Pf1N1B4_1038 0.50 +0.3 1,177,458 + Pf1N1B4_1038 0.50 +0.3 1,177,458 + Pf1N1B4_1038 0.50 +2.1 1,177,458 + Pf1N1B4_1038 0.50 +0.3 1,177,459 - Pf1N1B4_1038 0.50 -0.7 1,177,459 - Pf1N1B4_1038 0.50 -2.5 1,177,459 - Pf1N1B4_1038 0.50 -1.3 1,177,464 + +0.3 1,177,464 + Pf1N1B4_1038 0.51 -0.7 1,177,464 + Pf1N1B4_1038 0.51 -2.0 1,177,464 + Pf1N1B4_1038 0.51 +2.9 1,177,464 + Pf1N1B4_1038 0.51 +2.2 1,177,465 - Pf1N1B4_1038 0.51 +0.9 1,177,465 - Pf1N1B4_1038 0.51 -2.7 1,177,465 - Pf1N1B4_1038 0.51 +0.3 1,177,465 - Pf1N1B4_1038 0.51 -1.3 1,177,465 - Pf1N1B4_1038 0.51 +1.3 1,177,465 - Pf1N1B4_1038 0.51 +2.1 1,177,535 - Pf1N1B4_1038 0.58 +1.3 1,177,535 - Pf1N1B4_1038 0.58 +0.3 1,177,603 + Pf1N1B4_1038 0.65 -0.8 1,177,672 - Pf1N1B4_1038 0.73 -0.3 1,177,708 - Pf1N1B4_1038 0.76 -0.7 1,177,708 - Pf1N1B4_1038 0.76 +1.9 1,177,753 + Pf1N1B4_1038 0.81 +0.3 1,177,754 - Pf1N1B4_1038 0.81 -2.0 1,177,754 - Pf1N1B4_1038 0.81 -0.7 1,177,788 + Pf1N1B4_1038 0.85 -2.3 1,177,789 - Pf1N1B4_1038 0.85 +3.0 1,177,789 - Pf1N1B4_1038 0.85 -0.7 1,177,818 + Pf1N1B4_1038 0.88 +0.3 1,177,942 + +0.1 1,177,942 + -1.0 1,177,942 + +1.1 1,177,942 + +0.9 1,177,943 - +0.9 1,177,943 - +0.3 1,177,952 + +0.7 1,177,952 + -0.6 1,177,952 + -0.3 1,177,953 - -0.7 1,178,010 + +0.1 1,178,011 - +0.3 1,178,105 - -1.7 1,178,146 + -1.7 1,178,147 - -1.3 1,178,272 - Pf1N1B4_1039 0.23 +0.3 1,178,308 + Pf1N1B4_1039 0.29 -0.7 1,178,309 - Pf1N1B4_1039 0.29 -1.7 1,178,309 - Pf1N1B4_1039 0.29 +0.3 1,178,532 + Pf1N1B4_1039 0.69 -0.7 1,178,533 - Pf1N1B4_1039 0.69 -0.1 1,178,612 + Pf1N1B4_1039 0.83 -1.1 1,178,612 + Pf1N1B4_1039 0.83 -0.9 1,178,613 - Pf1N1B4_1039 0.83 -0.7 1,178,613 - Pf1N1B4_1039 0.83 -0.3 1,178,613 - Pf1N1B4_1039 0.83 -1.3 1,178,613 - Pf1N1B4_1039 0.83 +1.9 1,178,613 - Pf1N1B4_1039 0.83 +0.7 1,178,718 + +0.3 1,178,719 - -1.7 1,178,719 - +2.6 1,178,742 + +1.3 1,178,742 + -1.3 1,178,742 + -0.3 1,178,742 + +0.3 1,178,742 + -2.0 1,178,743 - -1.3 1,178,743 - -1.1 1,178,743 - +0.3 1,178,743 - -1.7 1,178,743 - +2.4 1,178,743 - -2.5 1,178,743 - +0.6 1,178,743 - -0.6 1,178,767 - -0.7 1,178,769 - -0.7 1,178,769 - -0.7 1,178,781 - +3.1 1,178,808 + -0.7 1,178,809 - -1.0 1,179,120 + Pf1N1B4_1040 0.45 +0.3 1,179,120 + Pf1N1B4_1040 0.45 -1.1 1,179,120 + Pf1N1B4_1040 0.45 +0.9 1,179,121 - Pf1N1B4_1040 0.45 +0.2 1,179,121 - Pf1N1B4_1040 0.45 -1.3 1,179,121 - +0.3 1,179,338 + Pf1N1B4_1040 0.76 -0.7 1,179,338 + +0.3 1,179,339 - Pf1N1B4_1040 0.76 +1.3 1,179,427 + Pf1N1B4_1040 0.88 +2.9 1,179,427 + Pf1N1B4_1040 0.88 -1.3 1,179,427 + Pf1N1B4_1040 0.88 -1.3 1,179,427 + Pf1N1B4_1040 0.88 -2.3 1,179,428 - Pf1N1B4_1040 0.88 +1.0 1,179,428 - Pf1N1B4_1040 0.88 -1.3 1,179,428 - Pf1N1B4_1040 0.88 +0.3 1,179,428 - Pf1N1B4_1040 0.88 +1.6 1,179,428 - Pf1N1B4_1040 0.88 -0.3 1,179,442 + -1.7 1,179,460 + +0.1 1,179,460 + +0.6 1,179,460 + +1.7 1,179,461 - +0.6 1,179,461 - +0.3 1,179,461 - +0.7 1,179,508 + +0.3 1,179,508 + -1.0 1,179,508 + -0.3 1,179,508 + -1.7 1,179,508 + -0.9 1,179,541 - +0.3 1,179,573 - -0.6 1,179,659 + Pf1N1B4_1041 0.22 +1.3 1,179,659 + +0.3 1,179,659 + Pf1N1B4_1041 0.22 +1.5 1,179,660 - Pf1N1B4_1041 0.22 -2.7 1,179,660 - +0.3 1,179,660 - Pf1N1B4_1041 0.22 -1.7 1,179,660 - Pf1N1B4_1041 0.22 -0.7 1,179,674 + Pf1N1B4_1041 0.24 -1.3 1,179,675 - Pf1N1B4_1041 0.25 -1.3
Or see this region's nucleotide sequence