Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1012 and Pf1N1B4_1013 are separated by 135 nucleotides Pf1N1B4_1013 and Pf1N1B4_1014 are separated by 63 nucleotides
Pf1N1B4_1012 - Glycine cleavage system transcriptional activator, at 1,147,624 to 1,148,571
_1012
Pf1N1B4_1013 - L-serine ammonia-lyase (EC 4.3.1.17) (from data) , at 1,148,707 to 1,150,083
_1013
Pf1N1B4_1014 - Serine transporter, at 1,150,147 to 1,151,448
_1014
Position (kb)
1148
1149
1150
1151 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1147.866 kb on + strand, within Pf1N1B4_1012 at 1147.890 kb on + strand, within Pf1N1B4_1012 at 1147.891 kb on - strand, within Pf1N1B4_1012 at 1147.937 kb on + strand, within Pf1N1B4_1012 at 1147.937 kb on + strand, within Pf1N1B4_1012 at 1147.937 kb on + strand, within Pf1N1B4_1012 at 1147.938 kb on - strand, within Pf1N1B4_1012 at 1147.938 kb on - strand, within Pf1N1B4_1012 at 1147.938 kb on - strand, within Pf1N1B4_1012 at 1147.956 kb on - strand, within Pf1N1B4_1012 at 1147.961 kb on + strand, within Pf1N1B4_1012 at 1147.962 kb on - strand, within Pf1N1B4_1012 at 1148.071 kb on + strand at 1148.072 kb on - strand, within Pf1N1B4_1012 at 1148.072 kb on - strand, within Pf1N1B4_1012 at 1148.072 kb on - strand at 1148.423 kb on - strand, within Pf1N1B4_1012 at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.544 kb on + strand at 1148.544 kb on - strand at 1148.597 kb on + strand at 1148.694 kb on + strand at 1148.697 kb on - strand at 1148.857 kb on + strand, within Pf1N1B4_1013 at 1149.019 kb on - strand, within Pf1N1B4_1013 at 1149.019 kb on - strand, within Pf1N1B4_1013 at 1149.070 kb on + strand, within Pf1N1B4_1013 at 1149.070 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.136 kb on + strand, within Pf1N1B4_1013 at 1149.137 kb on - strand, within Pf1N1B4_1013 at 1149.218 kb on - strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.346 kb on - strand, within Pf1N1B4_1013 at 1149.346 kb on - strand, within Pf1N1B4_1013 at 1149.346 kb on - strand, within Pf1N1B4_1013 at 1149.530 kb on + strand, within Pf1N1B4_1013 at 1149.573 kb on + strand, within Pf1N1B4_1013 at 1149.573 kb on + strand, within Pf1N1B4_1013 at 1149.573 kb on + strand, within Pf1N1B4_1013 at 1149.574 kb on - strand, within Pf1N1B4_1013 at 1149.613 kb on + strand, within Pf1N1B4_1013 at 1149.613 kb on + strand, within Pf1N1B4_1013 at 1149.613 kb on + strand, within Pf1N1B4_1013 at 1149.613 kb on + strand, within Pf1N1B4_1013 at 1149.614 kb on - strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.617 kb on - strand, within Pf1N1B4_1013 at 1149.617 kb on - strand, within Pf1N1B4_1013 at 1149.788 kb on + strand, within Pf1N1B4_1013 at 1149.788 kb on + strand, within Pf1N1B4_1013 at 1149.788 kb on + strand, within Pf1N1B4_1013 at 1149.789 kb on - strand, within Pf1N1B4_1013 at 1149.972 kb on + strand at 1149.972 kb on + strand at 1149.972 kb on + strand at 1149.972 kb on + strand at 1149.972 kb on + strand at 1149.972 kb on + strand at 1149.973 kb on - strand at 1150.033 kb on + strand at 1150.033 kb on + strand at 1150.033 kb on + strand at 1150.033 kb on + strand at 1150.033 kb on + strand at 1150.033 kb on + strand at 1150.034 kb on - strand at 1150.108 kb on + strand at 1150.108 kb on + strand at 1150.109 kb on - strand at 1150.109 kb on - strand at 1150.109 kb on - strand at 1150.109 kb on - strand at 1150.109 kb on - strand at 1150.109 kb on - strand at 1150.128 kb on + strand at 1150.128 kb on + strand at 1150.128 kb on + strand at 1150.128 kb on + strand at 1150.129 kb on - strand at 1150.129 kb on - strand at 1150.132 kb on - strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.161 kb on + strand at 1150.161 kb on + strand at 1150.161 kb on + strand at 1150.161 kb on + strand at 1150.161 kb on + strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.187 kb on + strand at 1150.187 kb on + strand at 1150.187 kb on + strand at 1150.187 kb on + strand at 1150.188 kb on - strand at 1150.201 kb on - strand at 1150.201 kb on - strand at 1150.201 kb on - strand at 1150.201 kb on - strand at 1150.201 kb on - strand at 1150.201 kb on - strand at 1150.201 kb on - strand at 1150.211 kb on + strand at 1150.212 kb on - strand at 1150.261 kb on + strand at 1150.262 kb on - strand at 1150.262 kb on - strand at 1150.480 kb on + strand, within Pf1N1B4_1014 at 1150.480 kb on + strand, within Pf1N1B4_1014 at 1150.480 kb on + strand, within Pf1N1B4_1014 at 1150.481 kb on - strand, within Pf1N1B4_1014 at 1150.481 kb on - strand, within Pf1N1B4_1014 at 1150.513 kb on + strand, within Pf1N1B4_1014 at 1150.513 kb on + strand, within Pf1N1B4_1014 at 1150.513 kb on + strand, within Pf1N1B4_1014 at 1150.513 kb on + strand, within Pf1N1B4_1014 at 1150.514 kb on - strand, within Pf1N1B4_1014 at 1150.514 kb on - strand, within Pf1N1B4_1014 at 1151.029 kb on + strand, within Pf1N1B4_1014 at 1151.065 kb on + strand, within Pf1N1B4_1014 at 1151.066 kb on - strand, within Pf1N1B4_1014 at 1151.066 kb on - strand, within Pf1N1B4_1014
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 1,147,866 + Pf1N1B4_1012 0.26 +0.3 1,147,890 + Pf1N1B4_1012 0.28 -0.7 1,147,891 - Pf1N1B4_1012 0.28 +0.8 1,147,937 + Pf1N1B4_1012 0.33 +0.3 1,147,937 + Pf1N1B4_1012 0.33 +1.3 1,147,937 + Pf1N1B4_1012 0.33 -1.2 1,147,938 - Pf1N1B4_1012 0.33 +0.3 1,147,938 - Pf1N1B4_1012 0.33 +1.3 1,147,938 - Pf1N1B4_1012 0.33 -0.4 1,147,956 - Pf1N1B4_1012 0.35 -1.7 1,147,961 + Pf1N1B4_1012 0.36 +1.3 1,147,962 - Pf1N1B4_1012 0.36 +1.1 1,148,071 + +0.3 1,148,072 - Pf1N1B4_1012 0.47 -2.0 1,148,072 - Pf1N1B4_1012 0.47 -2.0 1,148,072 - -0.7 1,148,423 - Pf1N1B4_1012 0.84 +0.4 1,148,543 + -2.7 1,148,543 + +2.3 1,148,543 + -0.7 1,148,543 + -0.3 1,148,543 + +0.3 1,148,543 + +2.9 1,148,543 + +0.3 1,148,543 + -1.2 1,148,543 + -1.3 1,148,544 + -1.7 1,148,544 - -1.0 1,148,597 + +1.3 1,148,694 + +0.3 1,148,697 - -0.7 1,148,857 + Pf1N1B4_1013 0.11 -0.8 1,149,019 - Pf1N1B4_1013 0.23 +1.4 1,149,019 - Pf1N1B4_1013 0.23 -0.7 1,149,070 + Pf1N1B4_1013 0.26 +0.3 1,149,070 + Pf1N1B4_1013 0.26 +0.7 1,149,133 + Pf1N1B4_1013 0.31 -2.7 1,149,133 + Pf1N1B4_1013 0.31 -1.7 1,149,133 + Pf1N1B4_1013 0.31 -0.7 1,149,133 + Pf1N1B4_1013 0.31 -1.7 1,149,133 + Pf1N1B4_1013 0.31 -0.7 1,149,133 + Pf1N1B4_1013 0.31 -1.7 1,149,133 + Pf1N1B4_1013 0.31 -4.3 1,149,133 + Pf1N1B4_1013 0.31 -1.7 1,149,133 + Pf1N1B4_1013 0.31 -2.0 1,149,133 + Pf1N1B4_1013 0.31 +1.1 1,149,133 + Pf1N1B4_1013 0.31 -0.2 1,149,133 + Pf1N1B4_1013 0.31 -2.0 1,149,133 + Pf1N1B4_1013 0.31 +1.3 1,149,134 - Pf1N1B4_1013 0.31 -1.7 1,149,134 - Pf1N1B4_1013 0.31 -2.3 1,149,134 - Pf1N1B4_1013 0.31 +0.1 1,149,134 - Pf1N1B4_1013 0.31 -2.0 1,149,134 - Pf1N1B4_1013 0.31 -2.7 1,149,134 - Pf1N1B4_1013 0.31 +0.9 1,149,134 - Pf1N1B4_1013 0.31 -1.7 1,149,136 + Pf1N1B4_1013 0.31 -3.2 1,149,137 - Pf1N1B4_1013 0.31 +0.3 1,149,218 - Pf1N1B4_1013 0.37 -0.9 1,149,345 + Pf1N1B4_1013 0.46 -2.0 1,149,345 + Pf1N1B4_1013 0.46 -2.5 1,149,345 + Pf1N1B4_1013 0.46 -0.1 1,149,345 + Pf1N1B4_1013 0.46 -1.3 1,149,345 + Pf1N1B4_1013 0.46 -0.7 1,149,345 + Pf1N1B4_1013 0.46 -3.0 1,149,345 + Pf1N1B4_1013 0.46 -0.7 1,149,345 + Pf1N1B4_1013 0.46 -1.3 1,149,345 + Pf1N1B4_1013 0.46 -2.0 1,149,345 + Pf1N1B4_1013 0.46 -2.0 1,149,346 - Pf1N1B4_1013 0.46 -0.4 1,149,346 - Pf1N1B4_1013 0.46 -1.3 1,149,346 - Pf1N1B4_1013 0.46 -0.9 1,149,530 + Pf1N1B4_1013 0.60 -2.0 1,149,573 + Pf1N1B4_1013 0.63 -2.0 1,149,573 + Pf1N1B4_1013 0.63 -2.5 1,149,573 + Pf1N1B4_1013 0.63 -2.0 1,149,574 - Pf1N1B4_1013 0.63 -0.2 1,149,613 + Pf1N1B4_1013 0.66 -3.7 1,149,613 + Pf1N1B4_1013 0.66 -2.0 1,149,613 + Pf1N1B4_1013 0.66 +0.3 1,149,613 + Pf1N1B4_1013 0.66 -1.7 1,149,614 - Pf1N1B4_1013 0.66 -2.3 1,149,616 + Pf1N1B4_1013 0.66 -2.5 1,149,616 + Pf1N1B4_1013 0.66 +0.3 1,149,616 + Pf1N1B4_1013 0.66 -1.3 1,149,616 + Pf1N1B4_1013 0.66 -1.7 1,149,616 + Pf1N1B4_1013 0.66 -4.2 1,149,616 + Pf1N1B4_1013 0.66 -2.3 1,149,616 + Pf1N1B4_1013 0.66 +1.9 1,149,616 + Pf1N1B4_1013 0.66 -0.7 1,149,616 + Pf1N1B4_1013 0.66 +0.3 1,149,616 + Pf1N1B4_1013 0.66 -2.9 1,149,616 + Pf1N1B4_1013 0.66 -1.7 1,149,616 + Pf1N1B4_1013 0.66 -2.0 1,149,616 + Pf1N1B4_1013 0.66 +0.3 1,149,617 - Pf1N1B4_1013 0.66 -0.7 1,149,617 - Pf1N1B4_1013 0.66 +1.1 1,149,788 + Pf1N1B4_1013 0.79 -1.3 1,149,788 + Pf1N1B4_1013 0.79 -2.5 1,149,788 + Pf1N1B4_1013 0.79 -1.7 1,149,789 - Pf1N1B4_1013 0.79 -0.2 1,149,972 + -1.3 1,149,972 + -2.0 1,149,972 + -1.3 1,149,972 + -0.7 1,149,972 + -2.0 1,149,972 + -2.5 1,149,973 - +2.3 1,150,033 + -1.3 1,150,033 + -1.7 1,150,033 + -1.7 1,150,033 + -1.5 1,150,033 + -2.7 1,150,033 + +0.0 1,150,034 - -1.7 1,150,108 + +2.1 1,150,108 + -0.7 1,150,109 - -0.7 1,150,109 - -0.1 1,150,109 - -1.7 1,150,109 - +2.3 1,150,109 - -2.7 1,150,109 - -2.0 1,150,128 + +0.3 1,150,128 + +1.0 1,150,128 + +1.9 1,150,128 + +1.4 1,150,129 - +0.5 1,150,129 - +1.9 1,150,132 - -3.0 1,150,157 + -0.7 1,150,157 + +0.3 1,150,157 + -0.3 1,150,157 + +1.2 1,150,157 + -0.3 1,150,157 + -1.7 1,150,157 + +0.3 1,150,157 + -0.7 1,150,157 + -2.3 1,150,157 + -0.3 1,150,157 + +0.3 1,150,157 + -1.6 1,150,157 + -0.7 1,150,157 + -2.3 1,150,157 + +0.6 1,150,157 + -0.1 1,150,157 + -0.1 1,150,158 - -0.7 1,150,158 - -1.3 1,150,158 - -1.3 1,150,158 - +1.0 1,150,158 - -2.3 1,150,158 - +0.3 1,150,158 - -0.3 1,150,158 - -2.0 1,150,158 - -0.9 1,150,158 - +0.6 1,150,158 - -2.9 1,150,158 - +0.7 1,150,158 - -0.9 1,150,158 - +0.3 1,150,158 - -0.7 1,150,158 - -0.3 1,150,158 - +1.2 1,150,161 + +0.8 1,150,161 + +0.6 1,150,161 + -0.7 1,150,161 + -2.0 1,150,161 + +1.9 1,150,162 - -0.1 1,150,162 - +0.3 1,150,162 - -1.9 1,150,162 - -0.1 1,150,162 - +0.5 1,150,162 - +1.6 1,150,162 - -1.0 1,150,162 - -0.7 1,150,162 - -0.4 1,150,162 - -0.7 1,150,187 + +0.3 1,150,187 + +0.3 1,150,187 + +1.7 1,150,187 + -0.2 1,150,188 - +1.3 1,150,201 - +0.0 1,150,201 - -2.0 1,150,201 - +0.9 1,150,201 - -1.7 1,150,201 - +0.1 1,150,201 - -0.3 1,150,201 - -0.1 1,150,211 + -1.4 1,150,212 - +1.1 1,150,261 + -1.7 1,150,262 - -2.0 1,150,262 - -0.7 1,150,480 + Pf1N1B4_1014 0.26 -0.7 1,150,480 + Pf1N1B4_1014 0.26 -0.3 1,150,480 + Pf1N1B4_1014 0.26 +0.6 1,150,481 - Pf1N1B4_1014 0.26 -0.7 1,150,481 - Pf1N1B4_1014 0.26 -0.9 1,150,513 + Pf1N1B4_1014 0.28 -1.3 1,150,513 + Pf1N1B4_1014 0.28 -1.0 1,150,513 + Pf1N1B4_1014 0.28 -1.3 1,150,513 + Pf1N1B4_1014 0.28 +1.0 1,150,514 - Pf1N1B4_1014 0.28 +2.9 1,150,514 - Pf1N1B4_1014 0.28 -2.0 1,151,029 + Pf1N1B4_1014 0.68 -1.7 1,151,065 + Pf1N1B4_1014 0.71 -1.9 1,151,066 - Pf1N1B4_1014 0.71 -0.4 1,151,066 - Pf1N1B4_1014 0.71 +1.3
Or see this region's nucleotide sequence