Experiment: Recovery broth with Flavomycin 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14975 and BBR_RS14980 are separated by 147 nucleotides BBR_RS14980 and BBR_RS14985 are separated by 244 nucleotides
BBR_RS14975: BBR_RS14975 - purine permease, at 1,132,158 to 1,133,522
_RS14975
BBR_RS14980: BBR_RS14980 - xanthine phosphoribosyltransferase, at 1,133,670 to 1,134,251
_RS14980
BBR_RS14985: BBR_RS14985 - ATP-dependent DNA helicase PcrA, at 1,134,496 to 1,137,171
_RS14985
Position (kb)
1133
1134
1135 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1132.692 kb on + strand, within BBR_RS14975 at 1132.700 kb on - strand, within BBR_RS14975 at 1132.700 kb on - strand, within BBR_RS14975 at 1132.700 kb on - strand, within BBR_RS14975 at 1132.722 kb on + strand, within BBR_RS14975 at 1132.733 kb on + strand, within BBR_RS14975 at 1132.750 kb on + strand, within BBR_RS14975 at 1132.750 kb on + strand, within BBR_RS14975 at 1132.750 kb on + strand, within BBR_RS14975 at 1132.758 kb on - strand, within BBR_RS14975 at 1132.758 kb on - strand, within BBR_RS14975 at 1132.758 kb on - strand, within BBR_RS14975 at 1132.768 kb on - strand, within BBR_RS14975 at 1132.768 kb on - strand, within BBR_RS14975 at 1132.836 kb on + strand, within BBR_RS14975 at 1132.872 kb on + strand, within BBR_RS14975 at 1132.910 kb on - strand, within BBR_RS14975 at 1133.017 kb on - strand, within BBR_RS14975 at 1133.055 kb on - strand, within BBR_RS14975 at 1133.170 kb on + strand, within BBR_RS14975 at 1133.185 kb on + strand, within BBR_RS14975 at 1133.185 kb on + strand, within BBR_RS14975 at 1133.193 kb on - strand, within BBR_RS14975 at 1133.242 kb on + strand, within BBR_RS14975 at 1133.256 kb on - strand, within BBR_RS14975 at 1133.286 kb on - strand, within BBR_RS14975 at 1133.288 kb on + strand, within BBR_RS14975 at 1133.289 kb on - strand, within BBR_RS14975 at 1133.296 kb on - strand, within BBR_RS14975 at 1133.343 kb on - strand, within BBR_RS14975 at 1133.358 kb on + strand, within BBR_RS14975 at 1133.361 kb on - strand, within BBR_RS14975 at 1133.544 kb on + strand at 1133.564 kb on - strand at 1133.587 kb on + strand at 1133.587 kb on - strand at 1133.648 kb on - strand at 1133.673 kb on - strand at 1133.675 kb on + strand at 1133.675 kb on + strand at 1133.675 kb on + strand at 1133.675 kb on + strand at 1133.680 kb on + strand at 1133.683 kb on - strand at 1133.683 kb on - strand at 1133.683 kb on - strand at 1133.794 kb on + strand, within BBR_RS14980 at 1133.832 kb on - strand, within BBR_RS14980 at 1133.832 kb on - strand, within BBR_RS14980 at 1133.928 kb on + strand, within BBR_RS14980 at 1133.953 kb on - strand, within BBR_RS14980 at 1133.953 kb on - strand, within BBR_RS14980 at 1133.955 kb on + strand, within BBR_RS14980 at 1133.955 kb on + strand, within BBR_RS14980 at 1133.990 kb on + strand, within BBR_RS14980 at 1134.029 kb on - strand, within BBR_RS14980 at 1134.182 kb on + strand, within BBR_RS14980 at 1134.213 kb on - strand at 1134.231 kb on - strand at 1134.256 kb on + strand at 1134.259 kb on + strand at 1134.314 kb on - strand at 1134.420 kb on - strand at 1134.420 kb on - strand at 1134.425 kb on - strand at 1134.425 kb on - strand at 1134.441 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Flavomycin 0.02 mM remove 1,132,692 + BBR_RS14975 0.39 +0.2 1,132,700 - BBR_RS14975 0.40 -0.8 1,132,700 - BBR_RS14975 0.40 +0.5 1,132,700 - BBR_RS14975 0.40 +2.6 1,132,722 + BBR_RS14975 0.41 -0.8 1,132,733 + BBR_RS14975 0.42 +0.1 1,132,750 + BBR_RS14975 0.43 +1.1 1,132,750 + BBR_RS14975 0.43 -2.6 1,132,750 + BBR_RS14975 0.43 -0.7 1,132,758 - BBR_RS14975 0.44 -0.3 1,132,758 - BBR_RS14975 0.44 +0.4 1,132,758 - BBR_RS14975 0.44 -0.3 1,132,768 - BBR_RS14975 0.45 +0.7 1,132,768 - BBR_RS14975 0.45 +0.5 1,132,836 + BBR_RS14975 0.50 -0.3 1,132,872 + BBR_RS14975 0.52 -0.9 1,132,910 - BBR_RS14975 0.55 +0.1 1,133,017 - BBR_RS14975 0.63 -0.2 1,133,055 - BBR_RS14975 0.66 +0.1 1,133,170 + BBR_RS14975 0.74 +0.6 1,133,185 + BBR_RS14975 0.75 +0.1 1,133,185 + BBR_RS14975 0.75 -0.4 1,133,193 - BBR_RS14975 0.76 -0.6 1,133,242 + BBR_RS14975 0.79 -0.1 1,133,256 - BBR_RS14975 0.80 -1.4 1,133,286 - BBR_RS14975 0.83 -0.1 1,133,288 + BBR_RS14975 0.83 -0.3 1,133,289 - BBR_RS14975 0.83 +0.9 1,133,296 - BBR_RS14975 0.83 -0.0 1,133,343 - BBR_RS14975 0.87 +0.1 1,133,358 + BBR_RS14975 0.88 -0.0 1,133,361 - BBR_RS14975 0.88 -0.1 1,133,544 + +0.9 1,133,564 - -0.0 1,133,587 + -0.6 1,133,587 - +0.4 1,133,648 - -0.4 1,133,673 - +0.3 1,133,675 + +0.1 1,133,675 + +0.3 1,133,675 + -0.3 1,133,675 + +0.6 1,133,680 + +0.4 1,133,683 - -0.7 1,133,683 - -0.0 1,133,683 - -0.5 1,133,794 + BBR_RS14980 0.21 +0.1 1,133,832 - BBR_RS14980 0.28 +1.9 1,133,832 - BBR_RS14980 0.28 -0.9 1,133,928 + BBR_RS14980 0.44 +0.6 1,133,953 - BBR_RS14980 0.49 -0.3 1,133,953 - BBR_RS14980 0.49 -0.7 1,133,955 + BBR_RS14980 0.49 -0.1 1,133,955 + BBR_RS14980 0.49 +1.3 1,133,990 + BBR_RS14980 0.55 +0.6 1,134,029 - BBR_RS14980 0.62 -0.6 1,134,182 + BBR_RS14980 0.88 +0.2 1,134,213 - -0.7 1,134,231 - +0.4 1,134,256 + +1.1 1,134,259 + +0.3 1,134,314 - -1.9 1,134,420 - +0.4 1,134,420 - -0.0 1,134,425 - -0.2 1,134,425 - +0.6 1,134,441 + -0.2
Or see this region's nucleotide sequence