Experiment: methylglyoxal 0.009 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_12560 and AO353_12565 are separated by 3 nucleotides AO353_12565 and AO353_12570 overlap by 8 nucleotides AO353_12570 and AO353_12575 overlap by 8 nucleotides AO353_12575 and AO353_12580 overlap by 4 nucleotides AO353_12580 and AO353_12585 overlap by 4 nucleotides
AO353_12560: AO353_12560 - type II secretion system protein GspG, at 2,769,367 to 2,769,822
_12560
AO353_12565: AO353_12565 - type II secretion system protein GspH, at 2,769,826 to 2,770,293
_12565
AO353_12570: AO353_12570 - type II secretion system protein GspI, at 2,770,286 to 2,770,678
_12570
AO353_12575: AO353_12575 - type II secretion system protein GspJ, at 2,770,671 to 2,771,216
_12575
AO353_12580: AO353_12580 - type II secretory protein PulK, at 2,771,213 to 2,772,067
_12580
AO353_12585: AO353_12585 - hypothetical protein, at 2,772,064 to 2,773,161
_12585
Position (kb)
2770
2771
2772 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2769.700 kb on + strand, within AO353_12560 at 2769.706 kb on + strand, within AO353_12560 at 2769.706 kb on + strand, within AO353_12560 at 2769.707 kb on - strand, within AO353_12560 at 2769.707 kb on - strand, within AO353_12560 at 2769.751 kb on + strand, within AO353_12560 at 2769.752 kb on - strand, within AO353_12560 at 2769.752 kb on - strand, within AO353_12560 at 2769.752 kb on - strand, within AO353_12560 at 2769.752 kb on - strand, within AO353_12560 at 2769.752 kb on - strand, within AO353_12560 at 2769.854 kb on + strand at 2769.854 kb on + strand at 2769.855 kb on - strand at 2769.855 kb on - strand at 2769.855 kb on - strand at 2769.855 kb on - strand at 2769.983 kb on + strand, within AO353_12565 at 2769.983 kb on + strand, within AO353_12565 at 2769.984 kb on - strand, within AO353_12565 at 2769.984 kb on - strand, within AO353_12565 at 2770.060 kb on + strand, within AO353_12565 at 2770.061 kb on - strand, within AO353_12565 at 2770.142 kb on + strand, within AO353_12565 at 2770.143 kb on - strand, within AO353_12565 at 2770.291 kb on + strand at 2770.291 kb on + strand at 2770.333 kb on + strand, within AO353_12570 at 2770.333 kb on + strand, within AO353_12570 at 2770.406 kb on + strand, within AO353_12570 at 2770.406 kb on + strand, within AO353_12570 at 2770.407 kb on - strand, within AO353_12570 at 2770.407 kb on - strand, within AO353_12570 at 2770.517 kb on + strand, within AO353_12570 at 2770.518 kb on - strand, within AO353_12570 at 2770.518 kb on - strand, within AO353_12570 at 2770.638 kb on + strand, within AO353_12570 at 2770.639 kb on - strand at 2770.639 kb on - strand at 2770.639 kb on - strand at 2770.639 kb on - strand at 2770.639 kb on - strand at 2770.639 kb on - strand at 2770.676 kb on + strand at 2770.676 kb on + strand at 2770.676 kb on + strand at 2770.677 kb on - strand at 2770.677 kb on - strand at 2770.677 kb on - strand at 2770.965 kb on + strand, within AO353_12575 at 2770.965 kb on + strand, within AO353_12575 at 2770.965 kb on + strand, within AO353_12575 at 2770.965 kb on + strand, within AO353_12575 at 2770.965 kb on + strand, within AO353_12575 at 2770.965 kb on + strand, within AO353_12575 at 2770.966 kb on - strand, within AO353_12575 at 2770.966 kb on - strand, within AO353_12575 at 2770.987 kb on + strand, within AO353_12575 at 2771.074 kb on - strand, within AO353_12575 at 2771.074 kb on - strand, within AO353_12575 at 2771.074 kb on - strand, within AO353_12575 at 2771.114 kb on + strand, within AO353_12575 at 2771.115 kb on - strand, within AO353_12575 at 2771.321 kb on - strand, within AO353_12580 at 2771.321 kb on - strand, within AO353_12580 at 2771.455 kb on + strand, within AO353_12580 at 2772.079 kb on + strand at 2772.079 kb on + strand at 2772.079 kb on + strand at 2772.079 kb on + strand at 2772.080 kb on - strand at 2772.147 kb on + strand at 2772.148 kb on - strand at 2772.188 kb on + strand, within AO353_12585 at 2772.188 kb on + strand, within AO353_12585 at 2772.189 kb on - strand, within AO353_12585
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.009 vol% remove 2,769,700 + AO353_12560 0.73 +2.5 2,769,706 + AO353_12560 0.74 -0.4 2,769,706 + AO353_12560 0.74 -0.8 2,769,707 - AO353_12560 0.75 -0.3 2,769,707 - AO353_12560 0.75 +0.7 2,769,751 + AO353_12560 0.84 +2.4 2,769,752 - AO353_12560 0.84 +0.9 2,769,752 - AO353_12560 0.84 -3.2 2,769,752 - AO353_12560 0.84 -0.2 2,769,752 - AO353_12560 0.84 -1.0 2,769,752 - AO353_12560 0.84 -2.6 2,769,854 + +0.8 2,769,854 + +2.1 2,769,855 - +0.0 2,769,855 - -0.0 2,769,855 - +0.4 2,769,855 - -2.1 2,769,983 + AO353_12565 0.34 -1.2 2,769,983 + AO353_12565 0.34 -0.1 2,769,984 - AO353_12565 0.34 +1.4 2,769,984 - AO353_12565 0.34 +2.1 2,770,060 + AO353_12565 0.50 -1.1 2,770,061 - AO353_12565 0.50 -0.6 2,770,142 + AO353_12565 0.68 +0.4 2,770,143 - AO353_12565 0.68 +0.8 2,770,291 + +1.4 2,770,291 + -1.5 2,770,333 + AO353_12570 0.12 +1.0 2,770,333 + AO353_12570 0.12 +2.3 2,770,406 + AO353_12570 0.31 +0.4 2,770,406 + AO353_12570 0.31 +0.4 2,770,407 - AO353_12570 0.31 -2.3 2,770,407 - AO353_12570 0.31 +0.3 2,770,517 + AO353_12570 0.59 -0.1 2,770,518 - AO353_12570 0.59 +0.4 2,770,518 - AO353_12570 0.59 -1.6 2,770,638 + AO353_12570 0.90 -1.0 2,770,639 - +2.0 2,770,639 - -0.2 2,770,639 - -1.5 2,770,639 - -2.1 2,770,639 - +2.8 2,770,639 - +0.5 2,770,676 + -0.2 2,770,676 + -0.2 2,770,676 + +1.9 2,770,677 - +0.2 2,770,677 - -0.2 2,770,677 - -1.2 2,770,965 + AO353_12575 0.54 +1.2 2,770,965 + AO353_12575 0.54 +2.9 2,770,965 + AO353_12575 0.54 -0.8 2,770,965 + AO353_12575 0.54 -0.7 2,770,965 + AO353_12575 0.54 -1.0 2,770,965 + AO353_12575 0.54 -1.0 2,770,966 - AO353_12575 0.54 +0.1 2,770,966 - AO353_12575 0.54 +1.6 2,770,987 + AO353_12575 0.58 +1.3 2,771,074 - AO353_12575 0.74 +0.3 2,771,074 - AO353_12575 0.74 -1.7 2,771,074 - AO353_12575 0.74 -0.8 2,771,114 + AO353_12575 0.81 +1.6 2,771,115 - AO353_12575 0.81 -1.7 2,771,321 - AO353_12580 0.13 -1.9 2,771,321 - AO353_12580 0.13 -2.0 2,771,455 + AO353_12580 0.28 -1.5 2,772,079 + +0.9 2,772,079 + -0.3 2,772,079 + +2.3 2,772,079 + +1.1 2,772,080 - +0.2 2,772,147 + -0.1 2,772,148 - -1.8 2,772,188 + AO353_12585 0.11 -1.7 2,772,188 + AO353_12585 0.11 +4.0 2,772,189 - AO353_12585 0.11 +2.3
Or see this region's nucleotide sequence