Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc04119 and SMc04118 are separated by 220 nucleotides SMc04118 and SMc04117 are separated by 7 nucleotides SMc04117 and SMc04116 are separated by 225 nucleotides
SMc04119: SMc04119 - phosphopentomutase, at 155,842 to 157,062
SMc04119
SMc04118: SMc04118 - hypothetical protein, at 157,283 to 157,867
SMc04118
SMc04117: SMc04117 - hypothetical protein, at 157,875 to 158,489
SMc04117
SMc04116: SMc04116 - transposase, at 158,715 to 159,878
SMc04116
Position (kb) 157
158
159 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 157.118 kb on + strand at 157.118 kb on + strand at 157.235 kb on - strand at 157.235 kb on - strand at 157.329 kb on + strand at 157.329 kb on + strand at 157.329 kb on + strand at 157.330 kb on - strand at 157.575 kb on + strand, within SMc04118 at 157.591 kb on - strand, within SMc04118 at 157.746 kb on + strand, within SMc04118 at 157.747 kb on - strand, within SMc04118 at 157.747 kb on - strand, within SMc04118 at 157.747 kb on - strand, within SMc04118 at 157.879 kb on + strand at 157.880 kb on - strand at 157.918 kb on + strand at 157.919 kb on - strand at 157.999 kb on + strand, within SMc04117 at 158.002 kb on + strand, within SMc04117 at 158.002 kb on + strand, within SMc04117 at 158.002 kb on + strand, within SMc04117 at 158.002 kb on + strand, within SMc04117 at 158.010 kb on + strand, within SMc04117 at 158.020 kb on + strand, within SMc04117 at 158.291 kb on + strand, within SMc04117 at 158.527 kb on + strand at 158.574 kb on - strand at 158.574 kb on - strand at 158.651 kb on + strand at 158.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 157,118 + -2.1 157,118 + +1.0 157,235 - -2.5 157,235 - -0.1 157,329 + -3.7 157,329 + -0.0 157,329 + +1.0 157,330 - -0.2 157,575 + SMc04118 0.50 -1.4 157,591 - SMc04118 0.53 +0.3 157,746 + SMc04118 0.79 +0.7 157,747 - SMc04118 0.79 -1.0 157,747 - SMc04118 0.79 -1.2 157,747 - SMc04118 0.79 -0.3 157,879 + +1.0 157,880 - +0.4 157,918 + -2.3 157,919 - +0.1 157,999 + SMc04117 0.20 +0.4 158,002 + SMc04117 0.21 +1.6 158,002 + SMc04117 0.21 +2.0 158,002 + SMc04117 0.21 -2.0 158,002 + SMc04117 0.21 +0.2 158,010 + SMc04117 0.22 +0.1 158,020 + SMc04117 0.24 +0.6 158,291 + SMc04117 0.68 -0.6 158,527 + -1.7 158,574 - +0.7 158,574 - +0.1 158,651 + -1.9 158,673 - -0.3
Or see this region's nucleotide sequence