Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc03010 and SMc03011 overlap by 1 nucleotides SMc03011 and SMc03012 overlap by 4 nucleotides SMc03012 and SMc03013 are separated by 29 nucleotides
SMc03010: SMc03010 - chemotaxis-specific methylesterase, at 708,133 to 709,182
SMc03010
SMc03011: SMc03011 - chemotaxis regulator protein, at 709,182 to 709,571
SMc03011
SMc03012: SMc03012 - chemoreceptor glutamine deamidase CheD, at 709,568 to 710,119
SMc03012
SMc03013: SMc03013 - hypothetical protein, at 710,149 to 710,523
SMc03013
Position (kb) 709
710 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 708.222 kb on + strand at 708.223 kb on - strand at 708.397 kb on - strand, within SMc03010 at 708.471 kb on + strand, within SMc03010 at 708.808 kb on + strand, within SMc03010 at 708.846 kb on + strand, within SMc03010 at 709.087 kb on + strand at 709.087 kb on + strand at 709.088 kb on - strand at 709.180 kb on + strand at 709.181 kb on - strand at 709.202 kb on - strand at 709.776 kb on - strand, within SMc03012 at 710.024 kb on + strand, within SMc03012 at 710.024 kb on + strand, within SMc03012 at 710.025 kb on - strand, within SMc03012 at 710.252 kb on + strand, within SMc03013 at 710.252 kb on + strand, within SMc03013 at 710.252 kb on + strand, within SMc03013 at 710.253 kb on - strand, within SMc03013 at 710.362 kb on - strand, within SMc03013
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 708,222 + +1.0 708,223 - -1.2 708,397 - SMc03010 0.25 +2.6 708,471 + SMc03010 0.32 +1.1 708,808 + SMc03010 0.64 +0.2 708,846 + SMc03010 0.68 -2.9 709,087 + -0.9 709,087 + +1.1 709,088 - -1.4 709,180 + +0.0 709,181 - -0.4 709,202 - +1.3 709,776 - SMc03012 0.38 +2.1 710,024 + SMc03012 0.83 -0.3 710,024 + SMc03012 0.83 +0.1 710,025 - SMc03012 0.83 +3.1 710,252 + SMc03013 0.27 +3.1 710,252 + SMc03013 0.27 -0.8 710,252 + SMc03013 0.27 +1.3 710,253 - SMc03013 0.28 -0.5 710,362 - SMc03013 0.57 -1.2
Or see this region's nucleotide sequence