Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02785 and SMc02784 overlap by 8 nucleotides SMc02784 and SMc02783 overlap by 4 nucleotides SMc02783 and SMc02782 are separated by 134 nucleotides
SMc02785: SMc02785 - lytic murein transglycosylase A protein, at 6,511 to 7,629
SMc02785
SMc02784: SMc02784 - hypothetical protein, at 7,622 to 8,188
SMc02784
SMc02783: SMc02783 - hypothetical protein, at 8,185 to 8,562
SMc02783
SMc02782: SMc02782 - DNA gyrase subunit B, at 8,697 to 11,132
SMc02782
Position (kb) 7
8
9 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 6.631 kb on + strand, within SMc02785 at 6.632 kb on - strand, within SMc02785 at 6.799 kb on + strand, within SMc02785 at 6.819 kb on - strand, within SMc02785 at 6.857 kb on - strand, within SMc02785 at 6.920 kb on - strand, within SMc02785 at 6.920 kb on - strand, within SMc02785 at 7.111 kb on + strand, within SMc02785 at 7.111 kb on + strand, within SMc02785 at 7.112 kb on - strand at 7.112 kb on - strand, within SMc02785 at 7.396 kb on + strand, within SMc02785 at 7.397 kb on - strand, within SMc02785 at 8.100 kb on + strand, within SMc02784 at 8.108 kb on + strand, within SMc02784 at 8.108 kb on + strand, within SMc02784 at 8.109 kb on - strand, within SMc02784 at 8.109 kb on - strand, within SMc02784 at 8.150 kb on + strand at 8.279 kb on - strand, within SMc02783 at 8.347 kb on + strand, within SMc02783 at 8.620 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 6,631 + SMc02785 0.11 -1.0 6,632 - SMc02785 0.11 +3.2 6,799 + SMc02785 0.26 -0.6 6,819 - SMc02785 0.28 +1.0 6,857 - SMc02785 0.31 +1.0 6,920 - SMc02785 0.37 -0.1 6,920 - SMc02785 0.37 -0.2 7,111 + SMc02785 0.54 +3.2 7,111 + SMc02785 0.54 -0.6 7,112 - +1.0 7,112 - SMc02785 0.54 +0.0 7,396 + SMc02785 0.79 +0.3 7,397 - SMc02785 0.79 -3.6 8,100 + SMc02784 0.84 -0.1 8,108 + SMc02784 0.86 +0.4 8,108 + SMc02784 0.86 +3.8 8,109 - SMc02784 0.86 +0.8 8,109 - SMc02784 0.86 +0.9 8,150 + -2.5 8,279 - SMc02783 0.25 -0.1 8,347 + SMc02783 0.43 -0.6 8,620 + +1.0
Or see this region's nucleotide sequence