Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02344 and SMc02345 are separated by 58 nucleotides SMc02345 and SMc02346 are separated by 11 nucleotides SMc02346 and SMc02347 are separated by 17 nucleotides
SMc02344: SMc02344 - periplasmic binding protein, at 2,767,271 to 2,768,305
SMc02344
SMc02345: SMc02345 - ABC transporter permease, at 2,768,364 to 2,769,227
SMc02345
SMc02346: SMc02346 - ABC transporter ATP-binding protein, at 2,769,239 to 2,770,075
SMc02346
SMc02347: SMc02347 - ferredoxin ASFB iron-sulfur protein, at 2,770,093 to 2,770,422
SMc02347
Position (kb)
2768
2769
2770 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2767.431 kb on + strand, within SMc02344 at 2767.886 kb on + strand, within SMc02344 at 2767.887 kb on - strand, within SMc02344 at 2768.016 kb on + strand, within SMc02344 at 2768.016 kb on + strand, within SMc02344 at 2768.016 kb on + strand, within SMc02344 at 2768.016 kb on + strand, within SMc02344 at 2768.017 kb on - strand, within SMc02344 at 2768.017 kb on - strand, within SMc02344 at 2768.311 kb on + strand at 2768.312 kb on - strand at 2768.312 kb on - strand at 2768.431 kb on + strand at 2768.431 kb on + strand at 2768.432 kb on - strand at 2768.476 kb on + strand, within SMc02345 at 2768.476 kb on + strand, within SMc02345 at 2768.477 kb on - strand, within SMc02345 at 2768.477 kb on - strand, within SMc02345 at 2769.126 kb on - strand, within SMc02345 at 2769.249 kb on + strand at 2769.250 kb on - strand at 2769.250 kb on - strand at 2769.250 kb on - strand at 2769.324 kb on + strand, within SMc02346 at 2769.444 kb on + strand, within SMc02346 at 2769.445 kb on - strand, within SMc02346 at 2769.445 kb on - strand, within SMc02346 at 2769.492 kb on - strand, within SMc02346 at 2769.492 kb on - strand, within SMc02346 at 2769.492 kb on - strand, within SMc02346 at 2769.504 kb on - strand, within SMc02346 at 2769.513 kb on - strand, within SMc02346 at 2769.578 kb on + strand, within SMc02346 at 2769.748 kb on - strand, within SMc02346 at 2769.748 kb on - strand, within SMc02346 at 2769.748 kb on - strand, within SMc02346 at 2769.789 kb on + strand, within SMc02346 at 2769.789 kb on + strand, within SMc02346 at 2769.804 kb on + strand at 2769.805 kb on - strand, within SMc02346 at 2769.805 kb on - strand, within SMc02346 at 2770.064 kb on + strand at 2770.064 kb on + strand at 2770.064 kb on + strand at 2770.065 kb on - strand at 2770.065 kb on - strand at 2770.065 kb on - strand at 2770.065 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 2,767,431 + SMc02344 0.15 +2.2 2,767,886 + SMc02344 0.59 -1.6 2,767,887 - SMc02344 0.60 +1.1 2,768,016 + SMc02344 0.72 -0.2 2,768,016 + SMc02344 0.72 +0.2 2,768,016 + SMc02344 0.72 +3.7 2,768,016 + SMc02344 0.72 -3.8 2,768,017 - SMc02344 0.72 +1.8 2,768,017 - SMc02344 0.72 +0.7 2,768,311 + -0.2 2,768,312 - -1.3 2,768,312 - -2.9 2,768,431 + -3.7 2,768,431 + -0.6 2,768,432 - -0.4 2,768,476 + SMc02345 0.13 -2.7 2,768,476 + SMc02345 0.13 -1.2 2,768,477 - SMc02345 0.13 -0.4 2,768,477 - SMc02345 0.13 +0.9 2,769,126 - SMc02345 0.88 +0.4 2,769,249 + +0.5 2,769,250 - +2.4 2,769,250 - +0.0 2,769,250 - -0.9 2,769,324 + SMc02346 0.10 -0.1 2,769,444 + SMc02346 0.24 -0.1 2,769,445 - SMc02346 0.25 +0.9 2,769,445 - SMc02346 0.25 +1.0 2,769,492 - SMc02346 0.30 +0.2 2,769,492 - SMc02346 0.30 +2.5 2,769,492 - SMc02346 0.30 -1.1 2,769,504 - SMc02346 0.32 -0.8 2,769,513 - SMc02346 0.33 -0.2 2,769,578 + SMc02346 0.41 -2.1 2,769,748 - SMc02346 0.61 +0.2 2,769,748 - SMc02346 0.61 +0.7 2,769,748 - SMc02346 0.61 -0.3 2,769,789 + SMc02346 0.66 +1.0 2,769,789 + SMc02346 0.66 +2.4 2,769,804 + +0.8 2,769,805 - SMc02346 0.68 -2.1 2,769,805 - SMc02346 0.68 -0.7 2,770,064 + -0.4 2,770,064 + -0.2 2,770,064 + +0.8 2,770,065 - -1.3 2,770,065 - -1.5 2,770,065 - +0.8 2,770,065 - +0.6
Or see this region's nucleotide sequence