Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02245 and SMc02246 overlap by 4 nucleotides SMc02246 and SMc02247 are separated by 120 nucleotides SMc02247 and SMc02248 are separated by 141 nucleotides SMc02248 and SMc02249 are separated by 156 nucleotides
SMc02245: SMc02245 - dihydroorotate dehydrogenase 2, at 608,536 to 609,624
SMc02245
SMc02246: SMc02246 - hypothetical protein, at 609,621 to 609,968
SMc02246
SMc02247: SMc02247 - signal peptide protein, at 610,089 to 610,526
SMc02247
SMc02248: SMc02248 - transcriptional regulator, at 610,668 to 611,315
SMc02248
SMc02249: SMc02249 - sensory transduction histidine kinase transmembrane protein, at 611,472 to 614,978
SMc02249
Position (kb) 610
611 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 609.167 kb on + strand, within SMc02245 at 609.167 kb on + strand, within SMc02245 at 609.208 kb on - strand, within SMc02245 at 609.280 kb on - strand, within SMc02245 at 609.930 kb on + strand, within SMc02246 at 609.952 kb on + strand at 609.952 kb on + strand at 609.952 kb on + strand at 609.952 kb on + strand at 609.952 kb on + strand at 609.953 kb on - strand at 609.953 kb on - strand at 609.953 kb on - strand at 609.954 kb on + strand at 609.955 kb on - strand at 609.959 kb on + strand at 610.176 kb on + strand, within SMc02247 at 610.596 kb on + strand at 610.596 kb on + strand at 610.597 kb on - strand at 610.597 kb on - strand at 611.187 kb on + strand, within SMc02248 at 611.285 kb on - strand at 611.474 kb on - strand at 611.474 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 609,167 + SMc02245 0.58 +2.7 609,167 + SMc02245 0.58 -0.5 609,208 - SMc02245 0.62 +0.8 609,280 - SMc02245 0.68 +0.3 609,930 + SMc02246 0.89 +1.2 609,952 + +1.1 609,952 + +1.7 609,952 + +0.6 609,952 + +1.3 609,952 + -3.2 609,953 - -0.2 609,953 - -3.4 609,953 - +2.9 609,954 + -2.7 609,955 - +1.3 609,959 + +0.4 610,176 + SMc02247 0.20 +1.3 610,596 + -0.0 610,596 + -1.4 610,597 - +0.2 610,597 - +1.0 611,187 + SMc02248 0.80 +0.8 611,285 - +0.9 611,474 - -3.4 611,474 - -0.5
Or see this region's nucleotide sequence