Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02049 and SMc02048 overlap by 1 nucleotides SMc02048 and SMc02047 are separated by 16 nucleotides SMc02047 and SMc01242 are separated by 874 nucleotides
SMc02049: SMc02049 - glycine dehydrogenase, at 1,670,379 to 1,673,243
SMc02049
SMc02048: SMc02048 - glycine cleavage system protein H, at 1,673,243 to 1,673,605
SMc02048
SMc02047: SMc02047 - glycine cleavage system aminomethyltransferase T, at 1,673,622 to 1,674,761
SMc02047
SMc01242: SMc01242 - signal peptide protein, at 1,675,636 to 1,676,616
SMc01242
Position (kb)
1673
1674
1675 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1672.843 kb on + strand, within SMc02049 at 1672.843 kb on + strand, within SMc02049 at 1672.843 kb on + strand, within SMc02049 at 1672.889 kb on - strand, within SMc02049 at 1672.896 kb on - strand, within SMc02049 at 1672.896 kb on - strand, within SMc02049 at 1672.896 kb on - strand, within SMc02049 at 1672.906 kb on + strand, within SMc02049 at 1672.963 kb on + strand at 1673.107 kb on - strand at 1673.107 kb on - strand at 1673.209 kb on + strand at 1673.210 kb on - strand at 1673.210 kb on - strand at 1673.222 kb on + strand at 1673.415 kb on + strand, within SMc02048 at 1673.415 kb on + strand, within SMc02048 at 1673.416 kb on - strand, within SMc02048 at 1673.605 kb on + strand at 1673.605 kb on + strand at 1673.606 kb on - strand at 1673.632 kb on + strand at 1673.633 kb on - strand at 1673.677 kb on + strand at 1673.677 kb on + strand at 1673.678 kb on - strand at 1673.678 kb on - strand at 1673.678 kb on - strand at 1673.678 kb on - strand at 1673.737 kb on + strand, within SMc02047 at 1674.009 kb on - strand, within SMc02047 at 1674.032 kb on - strand, within SMc02047 at 1674.150 kb on + strand, within SMc02047 at 1674.176 kb on - strand, within SMc02047 at 1674.626 kb on + strand, within SMc02047 at 1674.861 kb on + strand at 1674.900 kb on + strand at 1674.901 kb on - strand at 1675.275 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,672,843 + SMc02049 0.86 -0.9 1,672,843 + SMc02049 0.86 +1.3 1,672,843 + SMc02049 0.86 -1.4 1,672,889 - SMc02049 0.88 -0.8 1,672,896 - SMc02049 0.88 +0.6 1,672,896 - SMc02049 0.88 +0.5 1,672,896 - SMc02049 0.88 +0.1 1,672,906 + SMc02049 0.88 -2.7 1,672,963 + -0.2 1,673,107 - -1.2 1,673,107 - +0.6 1,673,209 + +0.6 1,673,210 - -1.7 1,673,210 - -3.1 1,673,222 + +4.0 1,673,415 + SMc02048 0.47 +0.7 1,673,415 + SMc02048 0.47 +0.2 1,673,416 - SMc02048 0.48 +0.4 1,673,605 + +1.2 1,673,605 + -1.2 1,673,606 - -0.7 1,673,632 + -1.0 1,673,633 - +0.1 1,673,677 + -0.8 1,673,677 + +0.8 1,673,678 - +2.7 1,673,678 - -2.2 1,673,678 - -0.5 1,673,678 - +0.3 1,673,737 + SMc02047 0.10 +2.4 1,674,009 - SMc02047 0.34 +2.3 1,674,032 - SMc02047 0.36 +1.8 1,674,150 + SMc02047 0.46 -0.4 1,674,176 - SMc02047 0.49 +0.8 1,674,626 + SMc02047 0.88 -0.8 1,674,861 + +1.8 1,674,900 + -1.9 1,674,901 - -0.0 1,675,275 - +1.0
Or see this region's nucleotide sequence