Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01777 and SMc01778 overlap by 4 nucleotides SMc01778 and SMc01779 are separated by 6 nucleotides SMc01779 and SMc01780 are separated by 200 nucleotides
SMc01777: SMc01777 - 6,7-dimethyl-8-ribityllumazine synthase, at 1,322,709 to 1,323,170
SMc01777
SMc01778: SMc01778 - transcription antitermination protein NusB, at 1,323,167 to 1,323,649
SMc01778
SMc01779: SMc01779 - transport transmembrane protein, at 1,323,656 to 1,324,882
SMc01779
SMc01780: SMc01780 - membrane-bound proton-translocating pyrophosphatase, at 1,325,083 to 1,327,218
SMc01780
Position (kb)
1323
1324
1325 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1322.757 kb on + strand, within SMc01777 at 1322.823 kb on + strand, within SMc01777 at 1322.823 kb on + strand, within SMc01777 at 1322.823 kb on + strand, within SMc01777 at 1322.955 kb on + strand, within SMc01777 at 1323.086 kb on + strand, within SMc01777 at 1323.722 kb on - strand at 1324.076 kb on + strand, within SMc01779 at 1324.163 kb on + strand, within SMc01779 at 1324.364 kb on + strand, within SMc01779 at 1324.364 kb on + strand, within SMc01779 at 1324.365 kb on - strand, within SMc01779 at 1324.365 kb on - strand, within SMc01779 at 1324.365 kb on - strand, within SMc01779 at 1324.680 kb on - strand, within SMc01779 at 1324.680 kb on - strand, within SMc01779 at 1324.781 kb on + strand at 1324.782 kb on - strand at 1324.894 kb on - strand at 1325.065 kb on - strand at 1325.091 kb on - strand at 1325.091 kb on - strand at 1325.091 kb on - strand at 1325.091 kb on - strand at 1325.205 kb on + strand at 1325.227 kb on + strand at 1325.242 kb on + strand at 1325.259 kb on - strand at 1325.506 kb on + strand, within SMc01780 at 1325.509 kb on + strand, within SMc01780 at 1325.510 kb on - strand, within SMc01780 at 1325.510 kb on - strand, within SMc01780 at 1325.516 kb on + strand, within SMc01780 at 1325.516 kb on + strand, within SMc01780 at 1325.517 kb on - strand, within SMc01780 at 1325.517 kb on - strand, within SMc01780 at 1325.785 kb on + strand, within SMc01780 at 1325.785 kb on + strand, within SMc01780 at 1325.866 kb on + strand, within SMc01780
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,322,757 + SMc01777 0.10 +0.7 1,322,823 + SMc01777 0.25 -0.3 1,322,823 + SMc01777 0.25 +0.7 1,322,823 + SMc01777 0.25 -0.1 1,322,955 + SMc01777 0.53 -0.9 1,323,086 + SMc01777 0.82 +2.8 1,323,722 - +1.5 1,324,076 + SMc01779 0.34 -1.7 1,324,163 + SMc01779 0.41 +1.5 1,324,364 + SMc01779 0.58 -0.1 1,324,364 + SMc01779 0.58 -1.1 1,324,365 - SMc01779 0.58 -1.6 1,324,365 - SMc01779 0.58 +0.1 1,324,365 - SMc01779 0.58 -0.8 1,324,680 - SMc01779 0.83 -1.3 1,324,680 - SMc01779 0.83 +2.0 1,324,781 + -2.1 1,324,782 - +0.7 1,324,894 - -3.1 1,325,065 - +2.9 1,325,091 - +5.4 1,325,091 - -0.3 1,325,091 - -4.0 1,325,091 - -0.0 1,325,205 + -0.4 1,325,227 + -1.1 1,325,242 + -1.4 1,325,259 - -0.4 1,325,506 + SMc01780 0.20 -0.0 1,325,509 + SMc01780 0.20 -0.8 1,325,510 - SMc01780 0.20 -0.3 1,325,510 - SMc01780 0.20 -0.9 1,325,516 + SMc01780 0.20 +0.2 1,325,516 + SMc01780 0.20 -1.3 1,325,517 - SMc01780 0.20 -1.8 1,325,517 - SMc01780 0.20 +0.7 1,325,785 + SMc01780 0.33 -1.4 1,325,785 + SMc01780 0.33 -2.3 1,325,866 + SMc01780 0.37 +0.7
Or see this region's nucleotide sequence