Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc03949 and SMc01521 overlap by 4 nucleotides SMc01521 and SMc01520 are separated by 127 nucleotides SMc01520 and SMc01519 are separated by 5 nucleotides SMc01519 and SMc01748 are separated by 122 nucleotides SMc01748 and SMc01518 are separated by 114 nucleotides SMc01518 and SMc01517 are separated by 12 nucleotides
SMc03949: SMc03949 - nitrogen regulatory protein, at 2,611,440 to 2,611,712
SMc03949
SMc01521: SMc01521 - nitrogen regulatory protein, at 2,611,709 to 2,612,113
SMc01521
SMc01520: SMc01520 - hypothetical protein, at 2,612,241 to 2,612,633
SMc01520
SMc01519: SMc01519 - hypothetical protein, at 2,612,639 to 2,613,013
SMc01519
SMc01748: SMc01748 - hypothetical protein, at 2,613,136 to 2,613,396
SMc01748
SMc01518: SMc01518 - hypothetical protein, at 2,613,511 to 2,613,825
SMc01518
SMc01517: SMc01517 - hypothetical protein, at 2,613,838 to 2,614,104
SMc01517
Position (kb)
2612
2613
2614 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2611.683 kb on - strand, within SMc03949 at 2611.685 kb on - strand at 2611.685 kb on - strand at 2611.719 kb on - strand at 2611.811 kb on + strand, within SMc01521 at 2611.811 kb on + strand, within SMc01521 at 2611.812 kb on - strand, within SMc01521 at 2611.812 kb on - strand, within SMc01521 at 2611.845 kb on - strand, within SMc01521 at 2612.024 kb on + strand, within SMc01521 at 2612.024 kb on + strand, within SMc01521 at 2612.025 kb on - strand, within SMc01521 at 2612.025 kb on - strand, within SMc01521 at 2612.056 kb on + strand at 2612.057 kb on - strand, within SMc01521 at 2612.057 kb on - strand, within SMc01521 at 2612.086 kb on + strand at 2612.086 kb on + strand at 2612.087 kb on - strand at 2612.112 kb on - strand at 2612.139 kb on + strand at 2612.139 kb on + strand at 2612.269 kb on - strand at 2612.320 kb on - strand, within SMc01520 at 2612.320 kb on - strand, within SMc01520 at 2612.320 kb on - strand, within SMc01520 at 2612.320 kb on - strand, within SMc01520 at 2612.403 kb on + strand, within SMc01520 at 2612.404 kb on - strand, within SMc01520 at 2612.404 kb on - strand, within SMc01520 at 2612.442 kb on + strand, within SMc01520 at 2612.442 kb on + strand, within SMc01520 at 2612.442 kb on + strand, within SMc01520 at 2612.442 kb on + strand, within SMc01520 at 2612.442 kb on + strand, within SMc01520 at 2612.443 kb on - strand, within SMc01520 at 2612.616 kb on - strand at 2612.631 kb on + strand at 2612.631 kb on + strand at 2612.632 kb on - strand at 2612.632 kb on - strand at 2612.632 kb on - strand at 2612.880 kb on + strand, within SMc01519 at 2612.881 kb on + strand, within SMc01519 at 2612.881 kb on + strand, within SMc01519 at 2612.882 kb on - strand, within SMc01519 at 2612.882 kb on - strand, within SMc01519 at 2613.024 kb on - strand at 2613.054 kb on + strand at 2613.103 kb on + strand at 2613.103 kb on + strand at 2613.103 kb on + strand at 2613.103 kb on + strand at 2613.103 kb on + strand at 2613.104 kb on - strand at 2613.104 kb on - strand at 2613.135 kb on + strand at 2613.135 kb on + strand at 2613.136 kb on - strand at 2613.136 kb on - strand at 2613.144 kb on + strand at 2613.560 kb on + strand, within SMc01518 at 2613.560 kb on + strand, within SMc01518 at 2613.561 kb on - strand, within SMc01518 at 2613.561 kb on - strand, within SMc01518 at 2613.822 kb on - strand at 2613.822 kb on - strand at 2613.830 kb on + strand at 2613.831 kb on - strand at 2613.831 kb on - strand at 2613.839 kb on + strand at 2613.839 kb on + strand at 2613.892 kb on - strand, within SMc01517 at 2613.892 kb on - strand, within SMc01517 at 2613.892 kb on - strand, within SMc01517
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 2,611,683 - SMc03949 0.89 +1.7 2,611,685 - -0.3 2,611,685 - -0.1 2,611,719 - +0.8 2,611,811 + SMc01521 0.25 +2.0 2,611,811 + SMc01521 0.25 +0.8 2,611,812 - SMc01521 0.25 -1.2 2,611,812 - SMc01521 0.25 +1.8 2,611,845 - SMc01521 0.34 -2.6 2,612,024 + SMc01521 0.78 -2.0 2,612,024 + SMc01521 0.78 +2.9 2,612,025 - SMc01521 0.78 -1.8 2,612,025 - SMc01521 0.78 -1.2 2,612,056 + +1.8 2,612,057 - SMc01521 0.86 +1.4 2,612,057 - SMc01521 0.86 -3.0 2,612,086 + -2.9 2,612,086 + +1.9 2,612,087 - +0.6 2,612,112 - +0.5 2,612,139 + -3.6 2,612,139 + -2.3 2,612,269 - +1.2 2,612,320 - SMc01520 0.20 +1.3 2,612,320 - SMc01520 0.20 +0.2 2,612,320 - SMc01520 0.20 -3.1 2,612,320 - SMc01520 0.20 +0.7 2,612,403 + SMc01520 0.41 +2.4 2,612,404 - SMc01520 0.41 +1.0 2,612,404 - SMc01520 0.41 +0.1 2,612,442 + SMc01520 0.51 -0.5 2,612,442 + SMc01520 0.51 +0.3 2,612,442 + SMc01520 0.51 +0.9 2,612,442 + SMc01520 0.51 +1.0 2,612,442 + SMc01520 0.51 -0.6 2,612,443 - SMc01520 0.51 +1.2 2,612,616 - +1.0 2,612,631 + -0.6 2,612,631 + -0.4 2,612,632 - -2.3 2,612,632 - -0.9 2,612,632 - -0.2 2,612,880 + SMc01519 0.64 +0.5 2,612,881 + SMc01519 0.65 +1.4 2,612,881 + SMc01519 0.65 +0.9 2,612,882 - SMc01519 0.65 +0.8 2,612,882 - SMc01519 0.65 -0.3 2,613,024 - -0.7 2,613,054 + +0.4 2,613,103 + +1.0 2,613,103 + -3.9 2,613,103 + +0.8 2,613,103 + -0.5 2,613,103 + -0.6 2,613,104 - -0.2 2,613,104 - +0.5 2,613,135 + +0.5 2,613,135 + -0.2 2,613,136 - +0.2 2,613,136 - +0.8 2,613,144 + -2.2 2,613,560 + SMc01518 0.16 -0.4 2,613,560 + SMc01518 0.16 +1.8 2,613,561 - SMc01518 0.16 -0.6 2,613,561 - SMc01518 0.16 +3.8 2,613,822 - +1.3 2,613,822 - -1.0 2,613,830 + +0.8 2,613,831 - +0.1 2,613,831 - +0.5 2,613,839 + +1.7 2,613,839 + +1.6 2,613,892 - SMc01517 0.20 -0.2 2,613,892 - SMc01517 0.20 -0.7 2,613,892 - SMc01517 0.20 +0.2
Or see this region's nucleotide sequence