Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01422 and SMc01423 overlap by 14 nucleotides SMc01423 and SMc01424 overlap by 4 nucleotides SMc01424 and SMc01425 are separated by 67 nucleotides
SMc01422: SMc01422 - hypothetical protein, at 2,268,452 to 2,268,838
SMc01422
SMc01423: SMc01423 - nitrile hydratase subunit beta protein, at 2,268,825 to 2,269,484
SMc01423
SMc01424: SMc01424 - nitrile hydratase subunit alpha protein, at 2,269,481 to 2,270,122
SMc01424
SMc01425: SMc01425 - hypothetical protein, at 2,270,190 to 2,270,783
SMc01425
Position (kb)
2269
2270
2271 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2268.609 kb on + strand, within SMc01422 at 2268.609 kb on + strand, within SMc01422 at 2268.642 kb on + strand, within SMc01422 at 2268.687 kb on - strand, within SMc01422 at 2268.725 kb on + strand, within SMc01422 at 2268.827 kb on - strand at 2268.841 kb on + strand at 2268.842 kb on - strand at 2268.842 kb on - strand at 2268.869 kb on + strand at 2268.925 kb on + strand, within SMc01423 at 2268.925 kb on + strand, within SMc01423 at 2268.925 kb on + strand, within SMc01423 at 2268.926 kb on - strand, within SMc01423 at 2269.067 kb on + strand, within SMc01423 at 2269.279 kb on + strand, within SMc01423 at 2269.279 kb on + strand, within SMc01423 at 2269.279 kb on + strand, within SMc01423 at 2269.282 kb on + strand, within SMc01423 at 2269.283 kb on - strand, within SMc01423 at 2269.297 kb on + strand, within SMc01423 at 2269.298 kb on - strand, within SMc01423 at 2269.298 kb on - strand, within SMc01423 at 2269.605 kb on + strand, within SMc01424 at 2269.729 kb on - strand, within SMc01424 at 2269.764 kb on + strand, within SMc01424 at 2269.780 kb on + strand, within SMc01424 at 2270.154 kb on + strand at 2270.155 kb on - strand at 2270.168 kb on + strand at 2270.191 kb on + strand at 2270.371 kb on + strand, within SMc01425 at 2270.371 kb on + strand, within SMc01425 at 2270.371 kb on + strand, within SMc01425 at 2270.371 kb on + strand, within SMc01425 at 2270.372 kb on - strand, within SMc01425 at 2270.372 kb on - strand, within SMc01425 at 2270.372 kb on - strand, within SMc01425 at 2270.372 kb on - strand at 2270.372 kb on - strand, within SMc01425 at 2270.387 kb on + strand, within SMc01425 at 2270.387 kb on + strand, within SMc01425 at 2270.388 kb on - strand, within SMc01425 at 2270.389 kb on + strand, within SMc01425 at 2270.389 kb on + strand, within SMc01425 at 2270.539 kb on + strand, within SMc01425 at 2270.539 kb on + strand, within SMc01425 at 2270.540 kb on - strand, within SMc01425 at 2270.540 kb on - strand, within SMc01425 at 2270.567 kb on + strand, within SMc01425 at 2270.568 kb on - strand, within SMc01425 at 2270.569 kb on + strand, within SMc01425 at 2270.569 kb on + strand, within SMc01425 at 2270.570 kb on - strand, within SMc01425 at 2270.570 kb on - strand, within SMc01425 at 2270.657 kb on + strand, within SMc01425 at 2270.658 kb on - strand, within SMc01425 at 2270.658 kb on - strand, within SMc01425 at 2270.771 kb on + strand at 2270.772 kb on - strand at 2270.868 kb on + strand at 2270.868 kb on + strand at 2270.887 kb on - strand at 2270.923 kb on + strand at 2270.959 kb on - strand at 2271.040 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 2,268,609 + SMc01422 0.41 -0.2 2,268,609 + SMc01422 0.41 -1.4 2,268,642 + SMc01422 0.49 +0.8 2,268,687 - SMc01422 0.61 +0.0 2,268,725 + SMc01422 0.71 +0.2 2,268,827 - +0.3 2,268,841 + +1.1 2,268,842 - +0.9 2,268,842 - -1.8 2,268,869 + +1.5 2,268,925 + SMc01423 0.15 +0.7 2,268,925 + SMc01423 0.15 -0.4 2,268,925 + SMc01423 0.15 -1.5 2,268,926 - SMc01423 0.15 +0.5 2,269,067 + SMc01423 0.37 +0.9 2,269,279 + SMc01423 0.69 -2.7 2,269,279 + SMc01423 0.69 -2.0 2,269,279 + SMc01423 0.69 -2.7 2,269,282 + SMc01423 0.69 -1.6 2,269,283 - SMc01423 0.69 -0.2 2,269,297 + SMc01423 0.72 -0.6 2,269,298 - SMc01423 0.72 +0.3 2,269,298 - SMc01423 0.72 -0.4 2,269,605 + SMc01424 0.19 -0.2 2,269,729 - SMc01424 0.39 +1.6 2,269,764 + SMc01424 0.44 +1.0 2,269,780 + SMc01424 0.47 -1.6 2,270,154 + +0.8 2,270,155 - -1.0 2,270,168 + +1.7 2,270,191 + +1.0 2,270,371 + SMc01425 0.30 +0.3 2,270,371 + SMc01425 0.30 +1.6 2,270,371 + SMc01425 0.30 +0.9 2,270,371 + SMc01425 0.30 -2.8 2,270,372 - SMc01425 0.31 +0.0 2,270,372 - SMc01425 0.31 +1.1 2,270,372 - SMc01425 0.31 +0.8 2,270,372 - +0.8 2,270,372 - SMc01425 0.31 +0.1 2,270,387 + SMc01425 0.33 +0.6 2,270,387 + SMc01425 0.33 -0.1 2,270,388 - SMc01425 0.33 -0.5 2,270,389 + SMc01425 0.34 -0.0 2,270,389 + SMc01425 0.34 +1.4 2,270,539 + SMc01425 0.59 -0.8 2,270,539 + SMc01425 0.59 +0.1 2,270,540 - SMc01425 0.59 +0.5 2,270,540 - SMc01425 0.59 -2.4 2,270,567 + SMc01425 0.63 -1.4 2,270,568 - SMc01425 0.64 +0.9 2,270,569 + SMc01425 0.64 -3.5 2,270,569 + SMc01425 0.64 +1.1 2,270,570 - SMc01425 0.64 +0.3 2,270,570 - SMc01425 0.64 -0.2 2,270,657 + SMc01425 0.79 +0.8 2,270,658 - SMc01425 0.79 +0.7 2,270,658 - SMc01425 0.79 +4.4 2,270,771 + -1.8 2,270,772 - -1.8 2,270,868 + +2.2 2,270,868 + +1.2 2,270,887 - +1.3 2,270,923 + -3.0 2,270,959 - -0.3 2,271,040 + -0.2
Or see this region's nucleotide sequence