Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01147 and SMc01146 are separated by 11 nucleotides SMc01146 and SMc01145 are separated by 30 nucleotides SMc01145 and SMc01144 are separated by 236 nucleotides
SMc01147: SMc01147 - coproporphyrinogen III oxidase, at 404,075 to 405,256
SMc01147
SMc01146: SMc01146 - deoxyribonucleotide triphosphate pyrophosphatase, at 405,268 to 405,912
SMc01146
SMc01145: SMc01145 - hypothetical protein, at 405,943 to 406,356
SMc01145
SMc01144: SMc01144 - ribonuclease PH, at 406,593 to 407,312
SMc01144
Position (kb) 405
406 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 404.458 kb on - strand, within SMc01147 at 404.617 kb on - strand, within SMc01147 at 404.642 kb on - strand, within SMc01147 at 404.733 kb on + strand, within SMc01147 at 404.733 kb on + strand, within SMc01147 at 404.737 kb on + strand, within SMc01147 at 404.853 kb on - strand, within SMc01147 at 405.198 kb on + strand at 405.199 kb on - strand at 405.199 kb on - strand at 405.266 kb on - strand at 405.266 kb on - strand at 405.419 kb on + strand, within SMc01146 at 405.458 kb on + strand, within SMc01146 at 405.459 kb on - strand, within SMc01146 at 405.459 kb on - strand, within SMc01146 at 405.870 kb on + strand at 405.870 kb on + strand at 405.870 kb on + strand at 405.914 kb on + strand at 405.915 kb on - strand at 405.915 kb on - strand at 405.915 kb on - strand at 406.007 kb on + strand, within SMc01145 at 406.287 kb on - strand, within SMc01145 at 406.287 kb on - strand, within SMc01145 at 406.429 kb on - strand at 406.443 kb on - strand at 406.594 kb on + strand at 406.602 kb on + strand at 406.603 kb on - strand at 406.780 kb on + strand, within SMc01144 at 406.781 kb on - strand, within SMc01144 at 406.781 kb on - strand, within SMc01144
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 404,458 - SMc01147 0.32 -2.4 404,617 - SMc01147 0.46 +1.0 404,642 - SMc01147 0.48 -2.4 404,733 + SMc01147 0.56 -1.0 404,733 + SMc01147 0.56 -4.1 404,737 + SMc01147 0.56 -1.9 404,853 - SMc01147 0.66 -2.4 405,198 + -3.0 405,199 - -3.2 405,199 - -2.6 405,266 - -2.7 405,266 - -0.7 405,419 + SMc01146 0.23 -1.3 405,458 + SMc01146 0.29 -1.4 405,459 - SMc01146 0.30 -1.9 405,459 - SMc01146 0.30 +0.1 405,870 + -5.0 405,870 + +0.8 405,870 + -4.0 405,914 + -3.4 405,915 - -2.5 405,915 - -1.9 405,915 - +0.5 406,007 + SMc01145 0.15 -1.8 406,287 - SMc01145 0.83 +1.3 406,287 - SMc01145 0.83 -1.2 406,429 - +0.1 406,443 - -3.0 406,594 + -2.9 406,602 + -0.8 406,603 - +0.7 406,780 + SMc01144 0.26 -2.5 406,781 - SMc01144 0.26 -0.3 406,781 - SMc01144 0.26 -3.0
Or see this region's nucleotide sequence