Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00331 and SMc00435 are separated by 54 nucleotides SMc00435 and SMc00332 are separated by 245 nucleotides SMc00332 and SMc00333 are separated by 284 nucleotides
SMc00331: SMc00331 - hypothetical protein, at 284,921 to 285,928
SMc00331
SMc00435: SMc00435 - tRNA-Ala, at 285,983 to 286,058
SMc00435
SMc00332: SMc00332 - hypothetical protein, at 286,304 to 286,693
SMc00332
SMc00333: SMc00333 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 286,978 to 288,345
SMc00333
Position (kb) 285
286
287 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 285.118 kb on + strand, within SMc00331 at 285.193 kb on + strand, within SMc00331 at 285.195 kb on + strand, within SMc00331 at 285.195 kb on + strand, within SMc00331 at 285.303 kb on + strand, within SMc00331 at 285.304 kb on - strand, within SMc00331 at 285.811 kb on + strand, within SMc00331 at 285.811 kb on + strand, within SMc00331 at 285.812 kb on - strand, within SMc00331 at 285.833 kb on + strand at 285.834 kb on - strand at 285.889 kb on - strand at 285.907 kb on + strand at 285.946 kb on + strand at 285.947 kb on - strand at 285.947 kb on - strand at 285.964 kb on - strand at 286.179 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 285,118 + SMc00331 0.20 -2.6 285,193 + SMc00331 0.27 -1.5 285,195 + SMc00331 0.27 -2.1 285,195 + SMc00331 0.27 -2.7 285,303 + SMc00331 0.38 -2.0 285,304 - SMc00331 0.38 -3.9 285,811 + SMc00331 0.88 +3.2 285,811 + SMc00331 0.88 +0.3 285,812 - SMc00331 0.88 -3.2 285,833 + +3.6 285,834 - +0.8 285,889 - +1.7 285,907 + +0.1 285,946 + +1.2 285,947 - -1.3 285,947 - -0.7 285,964 - +0.2 286,179 - +1.3
Or see this region's nucleotide sequence