Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00419 and SMc00420 are separated by 185 nucleotides SMc00420 and SMc00421 are separated by 629 nucleotides
SMc00419: SMc00419 - glutathione synthetase, at 367,072 to 368,019
SMc00419
SMc00420: SMc00420 - hypothetical protein, at 368,205 to 369,737
SMc00420
SMc00421: SMc00421 - O-acetylserine sulfhydrylase A, at 370,367 to 371,335
SMc00421
Position (kb) 368
369
370 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 367.363 kb on + strand, within SMc00419 at 367.364 kb on - strand, within SMc00419 at 367.364 kb on - strand, within SMc00419 at 367.364 kb on - strand, within SMc00419 at 367.364 kb on - strand, within SMc00419 at 367.366 kb on - strand, within SMc00419 at 367.564 kb on + strand, within SMc00419 at 367.660 kb on + strand, within SMc00419 at 367.661 kb on - strand, within SMc00419 at 367.662 kb on + strand, within SMc00419 at 367.663 kb on - strand, within SMc00419 at 367.663 kb on - strand, within SMc00419 at 367.663 kb on - strand, within SMc00419 at 367.907 kb on - strand, within SMc00419 at 368.025 kb on - strand at 368.057 kb on - strand at 368.204 kb on + strand at 368.205 kb on - strand at 368.205 kb on - strand at 368.205 kb on - strand at 368.294 kb on + strand at 368.295 kb on - strand at 368.837 kb on + strand, within SMc00420 at 368.837 kb on + strand, within SMc00420 at 368.837 kb on + strand, within SMc00420 at 368.837 kb on + strand, within SMc00420 at 368.837 kb on + strand, within SMc00420 at 368.838 kb on - strand, within SMc00420 at 368.838 kb on - strand, within SMc00420 at 369.205 kb on - strand, within SMc00420 at 369.566 kb on - strand, within SMc00420 at 369.610 kb on - strand at 369.700 kb on - strand at 369.735 kb on + strand at 369.819 kb on - strand at 369.819 kb on - strand at 369.819 kb on - strand at 369.819 kb on - strand at 369.849 kb on - strand at 370.173 kb on - strand at 370.198 kb on + strand at 370.198 kb on + strand at 370.198 kb on + strand at 370.198 kb on + strand at 370.198 kb on + strand at 370.198 kb on + strand at 370.210 kb on + strand at 370.219 kb on + strand at 370.260 kb on - strand at 370.369 kb on - strand at 370.369 kb on - strand at 370.405 kb on - strand at 370.553 kb on + strand, within SMc00421 at 370.554 kb on - strand, within SMc00421 at 370.571 kb on + strand, within SMc00421 at 370.572 kb on - strand, within SMc00421 at 370.590 kb on + strand, within SMc00421 at 370.591 kb on - strand, within SMc00421 at 370.721 kb on - strand, within SMc00421
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 367,363 + SMc00419 0.31 +1.8 367,364 - SMc00419 0.31 +0.9 367,364 - SMc00419 0.31 +0.6 367,364 - SMc00419 0.31 +5.3 367,364 - SMc00419 0.31 -1.1 367,366 - SMc00419 0.31 -1.4 367,564 + SMc00419 0.52 -1.6 367,660 + SMc00419 0.62 +3.5 367,661 - SMc00419 0.62 +4.3 367,662 + SMc00419 0.62 +1.4 367,663 - SMc00419 0.62 +5.0 367,663 - SMc00419 0.62 +0.9 367,663 - SMc00419 0.62 +3.9 367,907 - SMc00419 0.88 +2.9 368,025 - +1.2 368,057 - +0.7 368,204 + +5.3 368,205 - +2.4 368,205 - +1.2 368,205 - +0.4 368,294 + -1.5 368,295 - +0.5 368,837 + SMc00420 0.41 +4.5 368,837 + SMc00420 0.41 -2.5 368,837 + SMc00420 0.41 -1.4 368,837 + SMc00420 0.41 -0.9 368,837 + SMc00420 0.41 +0.4 368,838 - SMc00420 0.41 -0.1 368,838 - SMc00420 0.41 -1.9 369,205 - SMc00420 0.65 -2.5 369,566 - SMc00420 0.89 -1.1 369,610 - +0.8 369,700 - +0.9 369,735 + -0.8 369,819 - +2.1 369,819 - +0.6 369,819 - +0.9 369,819 - -1.3 369,849 - +1.1 370,173 - +0.9 370,198 + -2.8 370,198 + +1.5 370,198 + +0.4 370,198 + -2.0 370,198 + -0.1 370,198 + -3.2 370,210 + -0.3 370,219 + +0.1 370,260 - -0.6 370,369 - -2.6 370,369 - -0.7 370,405 - -1.5 370,553 + SMc00421 0.19 +0.5 370,554 - SMc00421 0.19 +3.9 370,571 + SMc00421 0.21 -0.2 370,572 - SMc00421 0.21 +0.9 370,590 + SMc00421 0.23 -2.7 370,591 - SMc00421 0.23 -1.1 370,721 - SMc00421 0.37 -0.9
Or see this region's nucleotide sequence