Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00042 and SMc00106 are separated by 147 nucleotides SMc00106 and SMc00043 are separated by 232 nucleotides SMc00043 and SMc00107 are separated by 65 nucleotides SMc00107 and SMc00044 are separated by 127 nucleotides
SMc00042: SMc00042 - aminotransferase, at 1,065,361 to 1,066,242
SMc00042
SMc00106: SMc00106 - hypothetical protein, at 1,066,390 to 1,066,800
SMc00106
SMc00043: SMc00043 - superoxide dismutase Fe protein, at 1,067,033 to 1,067,635
SMc00043
SMc00107: SMc00107 - transcriptional regulator, at 1,067,701 to 1,068,414
SMc00107
SMc00044: SMc00044 - amino acid efflux protein, at 1,068,542 to 1,069,180
SMc00044
Position (kb)
1067
1068 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1066.123 kb on - strand, within SMc00042 at 1066.127 kb on - strand, within SMc00042 at 1066.127 kb on - strand, within SMc00042 at 1066.127 kb on - strand, within SMc00042 at 1066.207 kb on + strand at 1066.208 kb on - strand at 1066.257 kb on - strand at 1066.271 kb on - strand at 1066.272 kb on + strand at 1066.273 kb on - strand at 1066.273 kb on - strand at 1066.288 kb on - strand at 1066.391 kb on + strand at 1066.392 kb on - strand at 1066.496 kb on + strand, within SMc00106 at 1066.496 kb on + strand, within SMc00106 at 1066.500 kb on - strand, within SMc00106 at 1066.500 kb on - strand, within SMc00106 at 1066.500 kb on - strand, within SMc00106 at 1066.512 kb on - strand, within SMc00106 at 1066.628 kb on + strand, within SMc00106 at 1066.685 kb on + strand, within SMc00106 at 1066.845 kb on + strand at 1066.845 kb on + strand at 1066.845 kb on + strand at 1066.845 kb on + strand at 1066.845 kb on + strand at 1066.845 kb on + strand at 1066.846 kb on - strand at 1066.846 kb on - strand at 1066.846 kb on - strand at 1066.846 kb on - strand at 1066.846 kb on - strand at 1066.846 kb on - strand at 1066.851 kb on - strand at 1066.851 kb on - strand at 1066.851 kb on - strand at 1066.851 kb on - strand at 1066.851 kb on - strand at 1066.916 kb on + strand at 1066.978 kb on + strand at 1066.978 kb on + strand at 1066.978 kb on + strand at 1066.978 kb on + strand at 1066.978 kb on + strand at 1066.979 kb on - strand at 1067.633 kb on + strand at 1067.634 kb on - strand at 1067.646 kb on + strand at 1067.891 kb on + strand, within SMc00107 at 1067.891 kb on + strand, within SMc00107 at 1067.894 kb on + strand, within SMc00107 at 1068.237 kb on + strand, within SMc00107 at 1068.238 kb on - strand, within SMc00107 at 1068.239 kb on + strand, within SMc00107 at 1068.240 kb on - strand, within SMc00107 at 1068.240 kb on - strand, within SMc00107 at 1068.426 kb on + strand at 1068.469 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,066,123 - SMc00042 0.86 -0.6 1,066,127 - SMc00042 0.87 +1.3 1,066,127 - SMc00042 0.87 +0.0 1,066,127 - SMc00042 0.87 -1.1 1,066,207 + -0.9 1,066,208 - +0.5 1,066,257 - -1.2 1,066,271 - +2.1 1,066,272 + +2.2 1,066,273 - +0.8 1,066,273 - -1.6 1,066,288 - -3.4 1,066,391 + +2.2 1,066,392 - -2.1 1,066,496 + SMc00106 0.26 -0.9 1,066,496 + SMc00106 0.26 +3.0 1,066,500 - SMc00106 0.27 +0.4 1,066,500 - SMc00106 0.27 +1.5 1,066,500 - SMc00106 0.27 +0.1 1,066,512 - SMc00106 0.30 -1.2 1,066,628 + SMc00106 0.58 +1.9 1,066,685 + SMc00106 0.72 +0.7 1,066,845 + -0.4 1,066,845 + +0.5 1,066,845 + +0.2 1,066,845 + -1.8 1,066,845 + -0.2 1,066,845 + +0.9 1,066,846 - -0.3 1,066,846 - +0.3 1,066,846 - -0.7 1,066,846 - -0.9 1,066,846 - -1.2 1,066,846 - +0.8 1,066,851 - +0.1 1,066,851 - -0.5 1,066,851 - -2.9 1,066,851 - -0.9 1,066,851 - +1.0 1,066,916 + -0.2 1,066,978 + +1.0 1,066,978 + -1.2 1,066,978 + +0.5 1,066,978 + -0.9 1,066,978 + -1.2 1,066,979 - -0.5 1,067,633 + -0.3 1,067,634 - +5.5 1,067,646 + -0.6 1,067,891 + SMc00107 0.27 -1.4 1,067,891 + SMc00107 0.27 -1.9 1,067,894 + SMc00107 0.27 -0.8 1,068,237 + SMc00107 0.75 -0.4 1,068,238 - SMc00107 0.75 -1.2 1,068,239 + SMc00107 0.75 +0.8 1,068,240 - SMc00107 0.75 +1.6 1,068,240 - SMc00107 0.75 -1.7 1,068,426 + -0.9 1,068,469 - -0.5
Or see this region's nucleotide sequence