Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1483 and SMa5009 are separated by 60 nucleotides SMa5009 and SMa1485 overlap by 4 nucleotides
SMa1483: SMa1483 - dehydrogenase, at 815,138 to 817,558
SMa1483
SMa5009: SMa5009 - hypothetical protein, at 817,619 to 817,969
SMa5009
SMa1485: SMa1485 - Permease, MFS, at 817,966 to 819,255
SMa1485
Position (kb) 817
818 Strain fitness (log2 ratio) -2
-1
0
1
2 at 816.668 kb on - strand, within SMa1483 at 816.794 kb on + strand, within SMa1483 at 816.794 kb on + strand, within SMa1483 at 817.187 kb on + strand, within SMa1483 at 817.188 kb on - strand, within SMa1483 at 817.404 kb on - strand at 817.406 kb on - strand at 817.597 kb on - strand at 817.731 kb on + strand at 817.731 kb on + strand, within SMa5009 at 817.732 kb on - strand, within SMa5009 at 817.761 kb on + strand, within SMa5009 at 817.762 kb on - strand, within SMa5009 at 817.762 kb on - strand, within SMa5009 at 817.762 kb on - strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.764 kb on + strand, within SMa5009 at 817.765 kb on - strand, within SMa5009 at 817.834 kb on - strand, within SMa5009 at 817.858 kb on - strand, within SMa5009 at 817.858 kb on - strand, within SMa5009 at 817.973 kb on - strand at 818.420 kb on + strand, within SMa1485 at 818.420 kb on + strand, within SMa1485 at 818.753 kb on + strand, within SMa1485 at 818.754 kb on - strand, within SMa1485 at 818.754 kb on - strand, within SMa1485 at 818.940 kb on - strand, within SMa1485
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 816,668 - SMa1483 0.63 -2.0 816,794 + SMa1483 0.68 +1.6 816,794 + SMa1483 0.68 -2.4 817,187 + SMa1483 0.85 +0.6 817,188 - SMa1483 0.85 +0.8 817,404 - -0.3 817,406 - -0.4 817,597 - +0.2 817,731 + -1.4 817,731 + SMa5009 0.32 +0.7 817,732 - SMa5009 0.32 +0.6 817,761 + SMa5009 0.40 +1.2 817,762 - SMa5009 0.41 -0.3 817,762 - SMa5009 0.41 +0.6 817,762 - SMa5009 0.41 +2.1 817,764 + SMa5009 0.41 +1.0 817,764 + SMa5009 0.41 -1.2 817,764 + SMa5009 0.41 -0.5 817,764 + SMa5009 0.41 +1.7 817,764 + SMa5009 0.41 +1.1 817,764 + SMa5009 0.41 +0.3 817,764 + SMa5009 0.41 +1.0 817,765 - SMa5009 0.42 -1.4 817,834 - SMa5009 0.61 -0.1 817,858 - SMa5009 0.68 +0.1 817,858 - SMa5009 0.68 +0.9 817,973 - -2.7 818,420 + SMa1485 0.35 +0.5 818,420 + SMa1485 0.35 +1.1 818,753 + SMa1485 0.61 -0.1 818,754 - SMa1485 0.61 +1.2 818,754 - SMa1485 0.61 +0.9 818,940 - SMa1485 0.76 +0.0
Or see this region's nucleotide sequence