Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa2353 and SMa2355 are separated by 183 nucleotides
SMa2353: SMa2353 - oxidoreductase, at 1,324,660 to 1,326,858
SMa2353
SMa2355: SMa2355 - DNA polymerase IV, at 1,327,042 to 1,328,151
SMa2355
Position (kb)
1327
1328
1329 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1326.048 kb on + strand, within SMa2353 at 1326.048 kb on + strand, within SMa2353 at 1326.049 kb on - strand, within SMa2353 at 1326.073 kb on + strand, within SMa2353 at 1326.074 kb on - strand, within SMa2353 at 1326.074 kb on - strand, within SMa2353 at 1326.212 kb on + strand, within SMa2353 at 1326.212 kb on + strand, within SMa2353 at 1326.213 kb on - strand, within SMa2353 at 1326.214 kb on + strand, within SMa2353 at 1326.635 kb on - strand, within SMa2353 at 1326.774 kb on + strand at 1326.778 kb on + strand at 1326.778 kb on + strand at 1326.778 kb on + strand at 1326.778 kb on + strand at 1326.778 kb on + strand at 1326.778 kb on + strand at 1326.778 kb on + strand at 1326.779 kb on - strand at 1326.779 kb on - strand at 1326.779 kb on - strand at 1326.794 kb on + strand at 1326.795 kb on - strand at 1326.866 kb on - strand at 1326.866 kb on - strand at 1326.961 kb on - strand at 1326.961 kb on - strand at 1326.983 kb on + strand at 1326.984 kb on - strand at 1326.998 kb on + strand at 1326.999 kb on - strand at 1327.219 kb on + strand, within SMa2355 at 1327.220 kb on - strand, within SMa2355 at 1327.318 kb on + strand, within SMa2355 at 1327.361 kb on - strand, within SMa2355 at 1327.361 kb on - strand, within SMa2355 at 1327.399 kb on + strand, within SMa2355 at 1327.513 kb on + strand, within SMa2355 at 1327.514 kb on - strand, within SMa2355 at 1327.744 kb on + strand, within SMa2355 at 1327.745 kb on - strand, within SMa2355 at 1327.779 kb on + strand, within SMa2355 at 1327.825 kb on - strand, within SMa2355 at 1327.825 kb on - strand, within SMa2355 at 1328.249 kb on + strand at 1328.249 kb on + strand at 1328.334 kb on + strand at 1328.342 kb on + strand at 1328.343 kb on - strand at 1328.343 kb on - strand at 1328.345 kb on - strand at 1328.345 kb on - strand at 1328.345 kb on - strand at 1328.345 kb on - strand at 1328.345 kb on - strand at 1328.662 kb on - strand at 1328.673 kb on + strand at 1328.673 kb on + strand at 1328.693 kb on + strand at 1328.694 kb on - strand at 1328.742 kb on + strand at 1328.743 kb on - strand at 1328.763 kb on - strand at 1329.024 kb on + strand at 1329.024 kb on + strand at 1329.024 kb on + strand at 1329.025 kb on - strand at 1329.025 kb on - strand at 1329.025 kb on - strand at 1329.035 kb on - strand at 1329.049 kb on + strand at 1329.142 kb on + strand at 1329.143 kb on - strand at 1329.143 kb on - strand at 1329.143 kb on - strand at 1329.143 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,326,048 + SMa2353 0.63 -0.6 1,326,048 + SMa2353 0.63 +1.0 1,326,049 - SMa2353 0.63 +0.3 1,326,073 + SMa2353 0.64 -4.1 1,326,074 - SMa2353 0.64 -1.2 1,326,074 - SMa2353 0.64 -0.4 1,326,212 + SMa2353 0.71 -1.1 1,326,212 + SMa2353 0.71 +1.4 1,326,213 - SMa2353 0.71 +0.6 1,326,214 + SMa2353 0.71 +0.6 1,326,635 - SMa2353 0.90 -1.2 1,326,774 + +2.1 1,326,778 + +0.4 1,326,778 + -0.4 1,326,778 + -1.3 1,326,778 + +0.5 1,326,778 + -1.1 1,326,778 + -0.2 1,326,778 + -2.4 1,326,779 - -1.7 1,326,779 - +0.5 1,326,779 - -1.9 1,326,794 + +0.2 1,326,795 - -0.9 1,326,866 - +2.7 1,326,866 - -1.1 1,326,961 - -1.9 1,326,961 - -0.4 1,326,983 + +0.5 1,326,984 - +0.8 1,326,998 + +0.2 1,326,999 - +5.0 1,327,219 + SMa2355 0.16 -1.6 1,327,220 - SMa2355 0.16 -0.9 1,327,318 + SMa2355 0.25 -0.1 1,327,361 - SMa2355 0.29 -0.0 1,327,361 - SMa2355 0.29 +0.9 1,327,399 + SMa2355 0.32 -0.1 1,327,513 + SMa2355 0.42 -2.3 1,327,514 - SMa2355 0.43 +0.9 1,327,744 + SMa2355 0.63 -0.2 1,327,745 - SMa2355 0.63 -1.4 1,327,779 + SMa2355 0.66 +2.9 1,327,825 - SMa2355 0.71 +0.9 1,327,825 - SMa2355 0.71 -0.5 1,328,249 + -0.7 1,328,249 + -2.5 1,328,334 + -0.3 1,328,342 + +0.1 1,328,343 - +1.2 1,328,343 - -0.2 1,328,345 - -1.5 1,328,345 - +0.4 1,328,345 - +0.7 1,328,345 - -0.2 1,328,345 - -1.0 1,328,662 - +0.9 1,328,673 + +0.3 1,328,673 + -1.0 1,328,693 + -0.7 1,328,694 - -1.6 1,328,742 + +3.1 1,328,743 - -0.3 1,328,763 - -3.1 1,329,024 + -1.1 1,329,024 + +0.1 1,329,024 + +0.3 1,329,025 - +2.8 1,329,025 - -2.9 1,329,025 - -2.3 1,329,035 - -0.6 1,329,049 + -1.4 1,329,142 + -0.6 1,329,143 - -1.6 1,329,143 - +0.1 1,329,143 - -0.6 1,329,143 - -3.3
Or see this region's nucleotide sequence