Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1869 and SMa1871 are separated by 234 nucleotides SMa1871 and SMa1872 are separated by 5 nucleotides
SMa1869: SMa1869 - deaminase, at 1,060,923 to 1,062,248
SMa1869
SMa1871: SMa1871 - cyclodeaminase, at 1,062,483 to 1,063,475
SMa1871
SMa1872: SMa1872 - threonine dehydratase, at 1,063,481 to 1,064,452
SMa1872
Position (kb)
1062
1063
1064 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1061.977 kb on + strand, within SMa1869 at 1061.978 kb on - strand, within SMa1869 at 1061.978 kb on - strand, within SMa1869 at 1062.076 kb on + strand, within SMa1869 at 1062.155 kb on + strand at 1062.195 kb on + strand at 1062.356 kb on - strand at 1062.395 kb on + strand at 1062.396 kb on - strand at 1062.416 kb on - strand at 1062.469 kb on + strand at 1062.470 kb on - strand at 1062.514 kb on + strand at 1062.523 kb on + strand at 1062.564 kb on + strand at 1062.564 kb on + strand at 1062.605 kb on + strand, within SMa1871 at 1062.614 kb on - strand, within SMa1871 at 1062.614 kb on - strand, within SMa1871 at 1062.657 kb on + strand, within SMa1871 at 1062.661 kb on - strand, within SMa1871 at 1062.661 kb on - strand, within SMa1871 at 1062.741 kb on - strand, within SMa1871 at 1062.741 kb on - strand, within SMa1871 at 1062.745 kb on + strand, within SMa1871 at 1062.745 kb on + strand, within SMa1871 at 1062.746 kb on - strand, within SMa1871 at 1062.903 kb on - strand, within SMa1871 at 1063.085 kb on + strand, within SMa1871 at 1063.322 kb on - strand, within SMa1871 at 1063.322 kb on - strand, within SMa1871 at 1063.393 kb on + strand at 1063.393 kb on + strand at 1063.403 kb on + strand at 1063.403 kb on + strand at 1063.442 kb on + strand at 1063.765 kb on - strand, within SMa1872 at 1063.867 kb on + strand, within SMa1872 at 1063.867 kb on + strand, within SMa1872 at 1063.908 kb on - strand, within SMa1872 at 1063.975 kb on - strand, within SMa1872 at 1064.000 kb on - strand, within SMa1872 at 1064.114 kb on + strand, within SMa1872 at 1064.115 kb on - strand, within SMa1872 at 1064.358 kb on + strand at 1064.358 kb on + strand at 1064.358 kb on + strand at 1064.359 kb on - strand at 1064.359 kb on - strand at 1064.435 kb on + strand at 1064.435 kb on + strand at 1064.436 kb on - strand at 1064.436 kb on - strand at 1064.436 kb on - strand at 1064.443 kb on - strand at 1064.450 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,061,977 + SMa1869 0.79 +0.0 1,061,978 - SMa1869 0.80 -0.7 1,061,978 - SMa1869 0.80 -0.3 1,062,076 + SMa1869 0.87 +0.0 1,062,155 + -0.7 1,062,195 + -0.0 1,062,356 - -1.1 1,062,395 + +1.1 1,062,396 - +1.7 1,062,416 - -0.5 1,062,469 + -1.9 1,062,470 - +1.6 1,062,514 + -0.0 1,062,523 + +0.0 1,062,564 + -3.6 1,062,564 + +0.1 1,062,605 + SMa1871 0.12 -0.3 1,062,614 - SMa1871 0.13 +0.6 1,062,614 - SMa1871 0.13 +0.7 1,062,657 + SMa1871 0.18 -0.7 1,062,661 - SMa1871 0.18 -0.1 1,062,661 - SMa1871 0.18 +0.5 1,062,741 - SMa1871 0.26 +1.8 1,062,741 - SMa1871 0.26 +0.4 1,062,745 + SMa1871 0.26 -0.0 1,062,745 + SMa1871 0.26 +1.3 1,062,746 - SMa1871 0.26 +1.5 1,062,903 - SMa1871 0.42 +0.1 1,063,085 + SMa1871 0.61 +0.9 1,063,322 - SMa1871 0.84 +1.7 1,063,322 - SMa1871 0.84 -1.3 1,063,393 + -1.7 1,063,393 + -1.2 1,063,403 + -0.3 1,063,403 + -0.7 1,063,442 + -2.3 1,063,765 - SMa1872 0.29 -0.2 1,063,867 + SMa1872 0.40 -0.7 1,063,867 + SMa1872 0.40 -1.7 1,063,908 - SMa1872 0.44 +1.6 1,063,975 - SMa1872 0.51 -0.2 1,064,000 - SMa1872 0.53 +0.4 1,064,114 + SMa1872 0.65 -1.0 1,064,115 - SMa1872 0.65 +1.3 1,064,358 + +0.0 1,064,358 + -0.1 1,064,358 + +3.2 1,064,359 - -0.2 1,064,359 - +0.7 1,064,435 + +1.3 1,064,435 + -2.4 1,064,436 - +0.9 1,064,436 - -2.1 1,064,436 - +2.1 1,064,443 - +0.7 1,064,450 + +0.3
Or see this region's nucleotide sequence