Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1676 and SMa1677 are separated by 84 nucleotides SMa1677 and SMa1678 are separated by 280 nucleotides SMa1678 and SMa1680 are separated by 263 nucleotides
SMa1676: SMa1676 - hypothetical protein, at 940,014 to 941,072
SMa1676
SMa1677: SMa1677 - Metallo-dependent hydrolase, at 941,157 to 942,590
SMa1677
SMa1678: SMa1678 - hypothetical protein, at 942,871 to 943,152
SMa1678
SMa1680: SMa1680 - hypothetical protein, at 943,416 to 945,809
SMa1680
Position (kb) 941
942
943 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 940.166 kb on - strand, within SMa1676 at 940.244 kb on - strand, within SMa1676 at 940.375 kb on + strand, within SMa1676 at 940.375 kb on + strand, within SMa1676 at 940.375 kb on + strand, within SMa1676 at 940.375 kb on + strand at 940.375 kb on + strand, within SMa1676 at 940.376 kb on - strand, within SMa1676 at 940.376 kb on - strand, within SMa1676 at 940.493 kb on - strand, within SMa1676 at 940.615 kb on + strand, within SMa1676 at 940.615 kb on + strand, within SMa1676 at 940.615 kb on + strand, within SMa1676 at 940.616 kb on - strand, within SMa1676 at 940.652 kb on + strand, within SMa1676 at 940.653 kb on - strand, within SMa1676 at 940.680 kb on - strand, within SMa1676 at 940.769 kb on - strand, within SMa1676 at 940.769 kb on - strand, within SMa1676 at 940.833 kb on + strand, within SMa1676 at 940.833 kb on + strand, within SMa1676 at 940.834 kb on - strand, within SMa1676 at 940.898 kb on - strand, within SMa1676 at 940.910 kb on + strand, within SMa1676 at 940.952 kb on + strand, within SMa1676 at 940.953 kb on - strand, within SMa1676 at 941.044 kb on + strand at 941.045 kb on - strand at 941.078 kb on + strand at 941.079 kb on - strand at 941.079 kb on - strand at 941.109 kb on + strand at 941.395 kb on + strand, within SMa1677 at 941.418 kb on + strand, within SMa1677 at 941.419 kb on - strand, within SMa1677 at 941.489 kb on - strand, within SMa1677 at 941.499 kb on + strand, within SMa1677 at 941.500 kb on - strand, within SMa1677 at 941.506 kb on + strand, within SMa1677 at 941.507 kb on - strand, within SMa1677 at 941.530 kb on - strand, within SMa1677 at 941.737 kb on + strand, within SMa1677 at 941.737 kb on + strand, within SMa1677 at 941.737 kb on + strand, within SMa1677 at 941.737 kb on + strand, within SMa1677 at 941.737 kb on + strand, within SMa1677 at 941.738 kb on - strand, within SMa1677 at 941.738 kb on - strand, within SMa1677 at 941.738 kb on - strand, within SMa1677 at 941.845 kb on - strand, within SMa1677 at 942.037 kb on + strand, within SMa1677 at 942.037 kb on + strand, within SMa1677 at 942.037 kb on + strand, within SMa1677 at 942.037 kb on + strand, within SMa1677 at 942.037 kb on + strand, within SMa1677 at 942.038 kb on - strand, within SMa1677 at 942.116 kb on + strand, within SMa1677 at 942.325 kb on - strand, within SMa1677 at 942.693 kb on + strand at 942.792 kb on + strand at 942.793 kb on - strand at 942.801 kb on + strand at 942.801 kb on + strand at 942.802 kb on - strand at 942.802 kb on - strand at 942.819 kb on + strand at 942.855 kb on + strand at 942.860 kb on + strand at 942.860 kb on + strand at 942.861 kb on - strand at 942.861 kb on - strand at 942.862 kb on + strand at 942.864 kb on + strand at 942.864 kb on + strand at 942.865 kb on - strand at 942.865 kb on - strand at 942.872 kb on + strand at 943.353 kb on + strand at 943.354 kb on - strand at 943.354 kb on - strand at 943.417 kb on + strand at 943.443 kb on + strand at 943.525 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 940,166 - SMa1676 0.14 +1.1 940,244 - SMa1676 0.22 -1.9 940,375 + SMa1676 0.34 +1.6 940,375 + SMa1676 0.34 -1.9 940,375 + SMa1676 0.34 -1.0 940,375 + -1.4 940,375 + SMa1676 0.34 +0.3 940,376 - SMa1676 0.34 +0.1 940,376 - SMa1676 0.34 -0.1 940,493 - SMa1676 0.45 +1.6 940,615 + SMa1676 0.57 -0.8 940,615 + SMa1676 0.57 +1.0 940,615 + SMa1676 0.57 -0.2 940,616 - SMa1676 0.57 -1.7 940,652 + SMa1676 0.60 +2.1 940,653 - SMa1676 0.60 +0.9 940,680 - SMa1676 0.63 +2.1 940,769 - SMa1676 0.71 +1.3 940,769 - SMa1676 0.71 -0.6 940,833 + SMa1676 0.77 -0.7 940,833 + SMa1676 0.77 -0.1 940,834 - SMa1676 0.77 +0.9 940,898 - SMa1676 0.83 +0.2 940,910 + SMa1676 0.85 -1.3 940,952 + SMa1676 0.89 -0.5 940,953 - SMa1676 0.89 +1.1 941,044 + -1.4 941,045 - -0.0 941,078 + +1.5 941,079 - -0.7 941,079 - -0.7 941,109 + +0.9 941,395 + SMa1677 0.17 +1.1 941,418 + SMa1677 0.18 -3.4 941,419 - SMa1677 0.18 -1.7 941,489 - SMa1677 0.23 -0.9 941,499 + SMa1677 0.24 -3.7 941,500 - SMa1677 0.24 -0.6 941,506 + SMa1677 0.24 -0.8 941,507 - SMa1677 0.24 -0.7 941,530 - SMa1677 0.26 +1.5 941,737 + SMa1677 0.40 +1.2 941,737 + SMa1677 0.40 +0.6 941,737 + SMa1677 0.40 +0.8 941,737 + SMa1677 0.40 +0.5 941,737 + SMa1677 0.40 -3.6 941,738 - SMa1677 0.41 +1.9 941,738 - SMa1677 0.41 -1.4 941,738 - SMa1677 0.41 +0.2 941,845 - SMa1677 0.48 -0.2 942,037 + SMa1677 0.61 -0.2 942,037 + SMa1677 0.61 +3.4 942,037 + SMa1677 0.61 -4.0 942,037 + SMa1677 0.61 +0.5 942,037 + SMa1677 0.61 +1.9 942,038 - SMa1677 0.61 -2.9 942,116 + SMa1677 0.67 -0.2 942,325 - SMa1677 0.81 +1.1 942,693 + +2.0 942,792 + -0.5 942,793 - -1.9 942,801 + +1.0 942,801 + +0.4 942,802 - -1.2 942,802 - -2.6 942,819 + +1.6 942,855 + +1.8 942,860 + +0.6 942,860 + +2.3 942,861 - +2.1 942,861 - -1.7 942,862 + +1.7 942,864 + -0.3 942,864 + +1.8 942,865 - -0.1 942,865 - +0.3 942,872 + -0.1 943,353 + +3.5 943,354 - -0.7 943,354 - -2.0 943,417 + +0.1 943,443 + -2.8 943,525 + +0.3
Or see this region's nucleotide sequence