Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1589 and SMa1591 are separated by 494 nucleotides
SMa1589: SMa1589 - hypothetical protein, at 883,252 to 883,638
SMa1589
SMa1591: SMa1591 - CyaI3 adenylate/guanylate cyclase, at 884,133 to 887,612
SMa1591
Position (kb) 883
884 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 882.308 kb on + strand at 882.353 kb on - strand at 882.375 kb on - strand at 882.383 kb on + strand at 882.395 kb on + strand at 882.463 kb on - strand at 882.523 kb on + strand at 882.523 kb on + strand at 882.523 kb on + strand at 882.523 kb on + strand at 882.523 kb on + strand at 882.523 kb on + strand at 882.524 kb on - strand at 882.524 kb on - strand at 882.528 kb on - strand at 882.547 kb on + strand at 882.632 kb on + strand at 882.632 kb on + strand at 882.651 kb on + strand at 882.651 kb on + strand at 882.652 kb on - strand at 882.652 kb on - strand at 882.685 kb on + strand at 882.712 kb on + strand at 882.877 kb on + strand at 882.878 kb on - strand at 882.912 kb on + strand at 882.912 kb on + strand at 882.913 kb on - strand at 882.968 kb on + strand at 882.968 kb on + strand at 882.969 kb on - strand at 882.969 kb on - strand at 882.969 kb on - strand at 883.015 kb on + strand at 883.016 kb on - strand at 883.055 kb on + strand at 883.178 kb on + strand at 883.179 kb on - strand at 883.209 kb on + strand at 883.253 kb on + strand at 883.253 kb on + strand at 883.253 kb on + strand at 883.253 kb on + strand at 883.253 kb on + strand at 883.254 kb on - strand at 883.298 kb on - strand, within SMa1589 at 883.318 kb on + strand, within SMa1589 at 883.442 kb on - strand, within SMa1589 at 883.442 kb on - strand, within SMa1589 at 883.455 kb on + strand, within SMa1589 at 883.522 kb on + strand, within SMa1589 at 883.604 kb on + strand at 883.613 kb on + strand at 883.614 kb on - strand at 883.614 kb on - strand at 883.615 kb on + strand at 883.616 kb on - strand at 883.616 kb on - strand at 883.616 kb on - strand at 883.650 kb on + strand at 883.671 kb on - strand at 883.705 kb on + strand at 884.028 kb on - strand at 884.043 kb on - strand at 884.068 kb on + strand at 884.116 kb on + strand at 884.117 kb on - strand at 884.117 kb on - strand at 884.135 kb on - strand at 884.135 kb on - strand at 884.289 kb on + strand at 884.290 kb on - strand at 884.299 kb on - strand at 884.602 kb on - strand, within SMa1591
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 882,308 + +0.9 882,353 - +1.5 882,375 - +1.5 882,383 + +1.4 882,395 + -1.8 882,463 - +1.1 882,523 + +0.1 882,523 + -0.5 882,523 + -0.2 882,523 + -0.5 882,523 + +0.9 882,523 + +0.3 882,524 - -4.0 882,524 - +1.4 882,528 - -0.7 882,547 + -0.4 882,632 + +0.3 882,632 + +1.0 882,651 + -0.0 882,651 + -0.1 882,652 - -1.0 882,652 - -1.4 882,685 + -0.5 882,712 + +0.3 882,877 + -0.8 882,878 - -0.9 882,912 + +0.6 882,912 + -1.4 882,913 - +1.9 882,968 + -1.5 882,968 + +2.2 882,969 - -2.6 882,969 - -0.8 882,969 - +1.1 883,015 + -2.9 883,016 - +1.6 883,055 + -0.2 883,178 + -3.1 883,179 - +2.5 883,209 + +0.4 883,253 + -1.9 883,253 + -2.6 883,253 + +0.5 883,253 + -2.4 883,253 + -0.5 883,254 - -0.5 883,298 - SMa1589 0.12 -1.2 883,318 + SMa1589 0.17 -0.3 883,442 - SMa1589 0.49 -3.6 883,442 - SMa1589 0.49 +0.2 883,455 + SMa1589 0.52 -1.2 883,522 + SMa1589 0.70 -1.4 883,604 + -1.7 883,613 + +1.0 883,614 - -0.1 883,614 - -1.2 883,615 + -2.4 883,616 - -1.5 883,616 - -1.6 883,616 - +0.5 883,650 + -1.6 883,671 - -2.6 883,705 + -2.3 884,028 - +0.5 884,043 - +0.2 884,068 + -2.1 884,116 + -1.3 884,117 - -0.7 884,117 - -4.1 884,135 - +0.2 884,135 - +0.6 884,289 + +2.9 884,290 - +1.9 884,299 - +0.3 884,602 - SMa1591 0.13 -0.4
Or see this region's nucleotide sequence